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Microarray Analysis of the Gene Expression Profile and Lipid Metabolism in Fat-1 Transgenic Cattle.

Liu X, Bai C, Ding X, Wei Z, Guo H, Li G - PLoS ONE (2015)

Bottom Line: This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway.The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals.These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China; College of Animal Science and Animal Medicine, Tianjin Agriculture University, Tianjin, China.

ABSTRACT
Long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial for human health. However, humans and mammals are unable to synthesize n-3 PUFAs because they lack the n-3 desaturase gene fat-1 and must therefore obtain this type of fatty acid through their diet. Through the production of fat-1 transgenic animals, it is possible to obtain animal products that are rich in n-3 PUFAs, such as meat and milk. The aim of this study was to analyze the gene expression profile and the mechanism of lipid metabolism in fat-1 transgenic cattle and to accumulate important basic data that are required to obtain more efficient fat-1 transgenic cattle. Transcriptome profiling of fat-1 transgenic and wild-type cattle identified differentially expressed genes that are involved in 90 biological pathways, eight pathways of which were related to lipid metabolism processes 36 genes of which were related to lipid metabolism. This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway. These findings were verified by quantitative polymerase chain reaction. The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals. These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

No MeSH data available.


Related in: MedlinePlus

The hierarchical clustering analysis of fat-1 transgenic cattle and wild-type cattle.(A) The heat-map of the 2042 differentially expressed genes. (B) The heat-map of the top 1000 expressed genes. The columns and rows in the heat maps represent samples and genes, respectively. Sample names are displayed below the heat maps.
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pone.0138874.g001: The hierarchical clustering analysis of fat-1 transgenic cattle and wild-type cattle.(A) The heat-map of the 2042 differentially expressed genes. (B) The heat-map of the top 1000 expressed genes. The columns and rows in the heat maps represent samples and genes, respectively. Sample names are displayed below the heat maps.

Mentions: In this study, we compared the gene expression patterns of the fat-1 transgenic cattle with wild-type cattle. We used 43,711 transcript sequences as probes, and of these, 2042 were identified as being significantly differentially expressed (p<0.05, FDR<0.05), with a greater than 1.5-fold change in expression between the fat-1 transgenic cattle and wild-type cattle. Of the 2042 transcripts, there were 797 transcripts that were significantly up-regulated in the fat-1 transgenic cattle than in the wild-type cattle (Fig 1A, S2 and S3 Tables). Furthermore, we selected the top 1000 expressed genes for hierarchical clustering analysis and found that the transgenic cattle separated from the wild-type cattle, indicating the consistency of the genetic backgrounds for the three transgenic cattle (Fig 1B).


Microarray Analysis of the Gene Expression Profile and Lipid Metabolism in Fat-1 Transgenic Cattle.

Liu X, Bai C, Ding X, Wei Z, Guo H, Li G - PLoS ONE (2015)

The hierarchical clustering analysis of fat-1 transgenic cattle and wild-type cattle.(A) The heat-map of the 2042 differentially expressed genes. (B) The heat-map of the top 1000 expressed genes. The columns and rows in the heat maps represent samples and genes, respectively. Sample names are displayed below the heat maps.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591129&req=5

pone.0138874.g001: The hierarchical clustering analysis of fat-1 transgenic cattle and wild-type cattle.(A) The heat-map of the 2042 differentially expressed genes. (B) The heat-map of the top 1000 expressed genes. The columns and rows in the heat maps represent samples and genes, respectively. Sample names are displayed below the heat maps.
Mentions: In this study, we compared the gene expression patterns of the fat-1 transgenic cattle with wild-type cattle. We used 43,711 transcript sequences as probes, and of these, 2042 were identified as being significantly differentially expressed (p<0.05, FDR<0.05), with a greater than 1.5-fold change in expression between the fat-1 transgenic cattle and wild-type cattle. Of the 2042 transcripts, there were 797 transcripts that were significantly up-regulated in the fat-1 transgenic cattle than in the wild-type cattle (Fig 1A, S2 and S3 Tables). Furthermore, we selected the top 1000 expressed genes for hierarchical clustering analysis and found that the transgenic cattle separated from the wild-type cattle, indicating the consistency of the genetic backgrounds for the three transgenic cattle (Fig 1B).

Bottom Line: This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway.The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals.These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China; College of Animal Science and Animal Medicine, Tianjin Agriculture University, Tianjin, China.

ABSTRACT
Long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial for human health. However, humans and mammals are unable to synthesize n-3 PUFAs because they lack the n-3 desaturase gene fat-1 and must therefore obtain this type of fatty acid through their diet. Through the production of fat-1 transgenic animals, it is possible to obtain animal products that are rich in n-3 PUFAs, such as meat and milk. The aim of this study was to analyze the gene expression profile and the mechanism of lipid metabolism in fat-1 transgenic cattle and to accumulate important basic data that are required to obtain more efficient fat-1 transgenic cattle. Transcriptome profiling of fat-1 transgenic and wild-type cattle identified differentially expressed genes that are involved in 90 biological pathways, eight pathways of which were related to lipid metabolism processes 36 genes of which were related to lipid metabolism. This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway. These findings were verified by quantitative polymerase chain reaction. The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals. These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

No MeSH data available.


Related in: MedlinePlus