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Phenotypical and Genotypical Properties of an Arcanobacterium pluranimalium Strain Isolated from a Juvenile Giraffe (Giraffa camelopardalis reticulata).

Risse K, Schlez K, Eisenberg T, Geiger C, Balbutskaya A, Sammra O, Lämmler C, Abdulmawjood A - J Vet Med (2014)

Bottom Line: The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe.The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483(T) of 99.2%, 89.9%, and 99.1%, respectively.To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.

View Article: PubMed Central - PubMed

Affiliation: Landesbetrieb Hessisches Landeslabor, Schubertstraße 60, 35392 Gießen, Germany.

ABSTRACT
The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe. The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483(T) of 99.2%, 89.9%, and 99.1%, respectively. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.

No MeSH data available.


Related in: MedlinePlus

Dendrogram of sequences of gene pla of A. pluranimalium 4868 of the present study, three additional A. pluranimalium, and various other cytolytic toxin encoding genes obtained from GenBank.
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fig1: Dendrogram of sequences of gene pla of A. pluranimalium 4868 of the present study, three additional A. pluranimalium, and various other cytolytic toxin encoding genes obtained from GenBank.

Mentions: Sequencing 16S rDNA, the potentially cytolytic toxin pluranimaliumlysin encoding target gene pla and the glyceraldehyde-3-phosphate dehydrogenase encoding target gene gap revealed a sequence similarity of 99.2%, 89.9%, and 99.1% to the respective sequences of A. pluranimalium DSM 13483T. All three sequences of A. pluranimalium 4868 were deposited in GenBank (HG794511, HG423389, and HG423390). A typical dendrogram of the sequencing results of the genes pla and gap is shown in Figures 1 and 2. Comparable to gene plo of T. pyogenes, which appeared to be a constant characteristic of all investigated T. pyogenes [16–19], pla of A. pluranimalium seems to be also constantly present in all strains of this species and could be used, as described previously [8], and in the present study for molecular identification of A. pluranimalium. More recently, Moser et al. 2013 [20] also described pla as novel target for molecular identification of this species.


Phenotypical and Genotypical Properties of an Arcanobacterium pluranimalium Strain Isolated from a Juvenile Giraffe (Giraffa camelopardalis reticulata).

Risse K, Schlez K, Eisenberg T, Geiger C, Balbutskaya A, Sammra O, Lämmler C, Abdulmawjood A - J Vet Med (2014)

Dendrogram of sequences of gene pla of A. pluranimalium 4868 of the present study, three additional A. pluranimalium, and various other cytolytic toxin encoding genes obtained from GenBank.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590887&req=5

fig1: Dendrogram of sequences of gene pla of A. pluranimalium 4868 of the present study, three additional A. pluranimalium, and various other cytolytic toxin encoding genes obtained from GenBank.
Mentions: Sequencing 16S rDNA, the potentially cytolytic toxin pluranimaliumlysin encoding target gene pla and the glyceraldehyde-3-phosphate dehydrogenase encoding target gene gap revealed a sequence similarity of 99.2%, 89.9%, and 99.1% to the respective sequences of A. pluranimalium DSM 13483T. All three sequences of A. pluranimalium 4868 were deposited in GenBank (HG794511, HG423389, and HG423390). A typical dendrogram of the sequencing results of the genes pla and gap is shown in Figures 1 and 2. Comparable to gene plo of T. pyogenes, which appeared to be a constant characteristic of all investigated T. pyogenes [16–19], pla of A. pluranimalium seems to be also constantly present in all strains of this species and could be used, as described previously [8], and in the present study for molecular identification of A. pluranimalium. More recently, Moser et al. 2013 [20] also described pla as novel target for molecular identification of this species.

Bottom Line: The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe.The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483(T) of 99.2%, 89.9%, and 99.1%, respectively.To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.

View Article: PubMed Central - PubMed

Affiliation: Landesbetrieb Hessisches Landeslabor, Schubertstraße 60, 35392 Gießen, Germany.

ABSTRACT
The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe. The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483(T) of 99.2%, 89.9%, and 99.1%, respectively. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.

No MeSH data available.


Related in: MedlinePlus