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Frequency and Clinical Epidemiology of Canine Monocytic Ehrlichiosis in Dogs Infested with Ticks from Sinaloa, Mexico.

Sosa-Gutierrez CG, Quintero Martinez MT, Gaxiola Camacho SM, Cota Guajardo S, Esteve-Gassent MD, Gordillo-Pérez MG - J Vet Med (2013)

Bottom Line: The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count.The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%.The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatria, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, 07300 Mexico City, Mexico ; Departamento de Parasitologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de México, Mexico City, Mexico.

ABSTRACT
Ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. The prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. One hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from Sinaloa State (Mexico) were analyzed in this study. All animals were suspected of having Canine Monocytic Ehrlichiosis (CME). The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count. From all dogs blood samples analyzed, 74.3% were positive to E. canis by ELISA and 40.1% were positive by blood smear. The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%. In addition, thrombocytopenia was presented in 87.6% of positive dogs. The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae. Consequently, this is the first report in which the morulae were visualized in the blood samples, and E. canis-specific antibodies were detected in dogs from Sinaloa, Northwest of Mexico.

No MeSH data available.


Related in: MedlinePlus

Observed using light microscopy; this is an image created from a peripheral blood smear of a dog infected with Canine Monocytic Ehrlichiosis. This image shows the morulae within the cytoplasm of a monocyte. Wright's stain.
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Related In: Results  -  Collection


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fig1: Observed using light microscopy; this is an image created from a peripheral blood smear of a dog infected with Canine Monocytic Ehrlichiosis. This image shows the morulae within the cytoplasm of a monocyte. Wright's stain.

Mentions: Among the canine individuals with suspected CME 74.5% (113/152) have E. canis-specific antibodies. In addition, 40.1% (61/152) were found positive when examined by smear to detect E. canis morulae in monocytes (Figure 1). Overall the clinical manifestations observed (Table 1) were fever (91.2%), anorexia (86.7%), depression (85.0%), lethargy (72.6%), and petechiae (72.6%). The male-female ratio was 1 : 1, and the most affected age was 1-2 years old (53%), followed by under 1 year (28%) and 3–5 years (19%). We compare the results obtained from the blood smear technique and Snap 4DX; of the 152 samples analyzed; 60 (38.2%) and 21 (13.7%) were positive and negative for both methods diagnostically, with a sensibility of 78.8% (95% CI 69.8–85.6) and specificity 86.7% (95% CI 74.3–99.1), and were assessed by chi square test by calculating odds ratios (OR) and 95% confidence intervals (CI) with a 2.12 (95% CI 1.01–4.45) P < 0.05. The 87.6% (106) of the dogs with thrombocytopenia were positive to the presence of antibodies of E. canis and were evaluated by chi square test by calculating odds ratios (OD) and 95% confidence intervals (CI) with a 24.2 (95% CI 8.9–65.9) P < 0.01; this parameter is a risk factor for disease and showed high rates of exposure, consistent with previous reports [5].


Frequency and Clinical Epidemiology of Canine Monocytic Ehrlichiosis in Dogs Infested with Ticks from Sinaloa, Mexico.

Sosa-Gutierrez CG, Quintero Martinez MT, Gaxiola Camacho SM, Cota Guajardo S, Esteve-Gassent MD, Gordillo-Pérez MG - J Vet Med (2013)

Observed using light microscopy; this is an image created from a peripheral blood smear of a dog infected with Canine Monocytic Ehrlichiosis. This image shows the morulae within the cytoplasm of a monocyte. Wright's stain.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590880&req=5

fig1: Observed using light microscopy; this is an image created from a peripheral blood smear of a dog infected with Canine Monocytic Ehrlichiosis. This image shows the morulae within the cytoplasm of a monocyte. Wright's stain.
Mentions: Among the canine individuals with suspected CME 74.5% (113/152) have E. canis-specific antibodies. In addition, 40.1% (61/152) were found positive when examined by smear to detect E. canis morulae in monocytes (Figure 1). Overall the clinical manifestations observed (Table 1) were fever (91.2%), anorexia (86.7%), depression (85.0%), lethargy (72.6%), and petechiae (72.6%). The male-female ratio was 1 : 1, and the most affected age was 1-2 years old (53%), followed by under 1 year (28%) and 3–5 years (19%). We compare the results obtained from the blood smear technique and Snap 4DX; of the 152 samples analyzed; 60 (38.2%) and 21 (13.7%) were positive and negative for both methods diagnostically, with a sensibility of 78.8% (95% CI 69.8–85.6) and specificity 86.7% (95% CI 74.3–99.1), and were assessed by chi square test by calculating odds ratios (OR) and 95% confidence intervals (CI) with a 2.12 (95% CI 1.01–4.45) P < 0.05. The 87.6% (106) of the dogs with thrombocytopenia were positive to the presence of antibodies of E. canis and were evaluated by chi square test by calculating odds ratios (OD) and 95% confidence intervals (CI) with a 24.2 (95% CI 8.9–65.9) P < 0.01; this parameter is a risk factor for disease and showed high rates of exposure, consistent with previous reports [5].

Bottom Line: The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count.The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%.The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae.

View Article: PubMed Central - PubMed

Affiliation: Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatria, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, 07300 Mexico City, Mexico ; Departamento de Parasitologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de México, Mexico City, Mexico.

ABSTRACT
Ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. The prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. One hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from Sinaloa State (Mexico) were analyzed in this study. All animals were suspected of having Canine Monocytic Ehrlichiosis (CME). The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count. From all dogs blood samples analyzed, 74.3% were positive to E. canis by ELISA and 40.1% were positive by blood smear. The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%. In addition, thrombocytopenia was presented in 87.6% of positive dogs. The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae. Consequently, this is the first report in which the morulae were visualized in the blood samples, and E. canis-specific antibodies were detected in dogs from Sinaloa, Northwest of Mexico.

No MeSH data available.


Related in: MedlinePlus