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Acute Phase Response and Neutrophils : Lymphocyte Ratio in Response to Astaxanthin in Staphylococcal Mice Mastitis Model.

Dolma T, Mukherjee R, Pati BK, De UK - J Vet Med (2014)

Bottom Line: The CRP was positive in all the challenged group at 24 hrs PC, but it remained positive till 120 hrs PC in group II.The parameters are related to enhancement of the mammary defense and reduction of inflammation.Development of such therapy may be useful to reduce the antibiotic burden in management of intramammary infection.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.

ABSTRACT
The purpose of the study was to determine the immunotherapeutic effect of astaxanthin (AX) on total clinical score (TCS), C-reactive protein (CRP), and neutrophil : lymphocyte ratio in mice mastitis model challenged with pathogenic Staphylococcus aureus. Twenty-four lactating mice were divided in 4 equal groups: group I mice served as normal healthy control, group II, positive control, were challenged with pathogenic S. aureus, group III mice were challenged and treated with AX, and group IV were treated with amoxicillin plus sulbactum. The TCS was higher in postchallenged mice; however it was significantly higher in group II untreated mice as compared to group III and group IV mice. The neutrophil was higher and lymphocyte count was lower in group II mice at 120 hrs post challenge (PC). The CRP was positive in all the challenged group at 24 hrs PC, but it remained positive till 120 hrs PC in group II. The parameters are related to enhancement of the mammary defense and reduction of inflammation. Hence AX may be used alone or as an adjunct therapy with antibiotic for amelioration of mastitis. Development of such therapy may be useful to reduce the antibiotic burden in management of intramammary infection.

No MeSH data available.


Related in: MedlinePlus

Percent neutrophil and lymphocyte in blood in response to the treatment with astaxanthin (group III) and amoxicillin plus sulbactam (group IV) and in positive control (group II) and in normal healthy mice (group I) (mean ± SE).
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fig1: Percent neutrophil and lymphocyte in blood in response to the treatment with astaxanthin (group III) and amoxicillin plus sulbactam (group IV) and in positive control (group II) and in normal healthy mice (group I) (mean ± SE).

Mentions: DLC was done in all groups at 0 hr, 24 hrs, 48 h, 72 hrs, and 120 hrs post challenge, respectively. At 0 hr lymphocyte count was more and neutrophils count was lesser in all the 4 groups, whereas neutrophil count increased and lymphocyte count decreased significantly in all the infected groups (group II, group III, and group IV) as compared to healthy group (group I) at 24 hrs PC. In group II animals the mean neutrophil count increased to an extent of 66.07% at 120 hrs PC as compared to 0 hr count. Similarly in group III and group IV significantly higher count to an extent of 48.6% and 40.6% could be observed at 24 hrs, respectively; however the neutrophil count at 48 h, 72 hrs, and 120 hrs PC did not differ significantly, whereas the lymphocyte counts in group II animals decreased to an extent of 63% at 120 hrs PC as compared to 0 hr count. Similarly in group III and group IV significantly lower count to an extent of 18.9% and 31.6% could be observed at 24 hrs, respectively; however the lymphocyte count at 48 hrs, 72 hrs, and 120 hrs PC did not differ significantly (Figure 1).


Acute Phase Response and Neutrophils : Lymphocyte Ratio in Response to Astaxanthin in Staphylococcal Mice Mastitis Model.

Dolma T, Mukherjee R, Pati BK, De UK - J Vet Med (2014)

Percent neutrophil and lymphocyte in blood in response to the treatment with astaxanthin (group III) and amoxicillin plus sulbactam (group IV) and in positive control (group II) and in normal healthy mice (group I) (mean ± SE).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590875&req=5

fig1: Percent neutrophil and lymphocyte in blood in response to the treatment with astaxanthin (group III) and amoxicillin plus sulbactam (group IV) and in positive control (group II) and in normal healthy mice (group I) (mean ± SE).
Mentions: DLC was done in all groups at 0 hr, 24 hrs, 48 h, 72 hrs, and 120 hrs post challenge, respectively. At 0 hr lymphocyte count was more and neutrophils count was lesser in all the 4 groups, whereas neutrophil count increased and lymphocyte count decreased significantly in all the infected groups (group II, group III, and group IV) as compared to healthy group (group I) at 24 hrs PC. In group II animals the mean neutrophil count increased to an extent of 66.07% at 120 hrs PC as compared to 0 hr count. Similarly in group III and group IV significantly higher count to an extent of 48.6% and 40.6% could be observed at 24 hrs, respectively; however the neutrophil count at 48 h, 72 hrs, and 120 hrs PC did not differ significantly, whereas the lymphocyte counts in group II animals decreased to an extent of 63% at 120 hrs PC as compared to 0 hr count. Similarly in group III and group IV significantly lower count to an extent of 18.9% and 31.6% could be observed at 24 hrs, respectively; however the lymphocyte count at 48 hrs, 72 hrs, and 120 hrs PC did not differ significantly (Figure 1).

Bottom Line: The CRP was positive in all the challenged group at 24 hrs PC, but it remained positive till 120 hrs PC in group II.The parameters are related to enhancement of the mammary defense and reduction of inflammation.Development of such therapy may be useful to reduce the antibiotic burden in management of intramammary infection.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India.

ABSTRACT
The purpose of the study was to determine the immunotherapeutic effect of astaxanthin (AX) on total clinical score (TCS), C-reactive protein (CRP), and neutrophil : lymphocyte ratio in mice mastitis model challenged with pathogenic Staphylococcus aureus. Twenty-four lactating mice were divided in 4 equal groups: group I mice served as normal healthy control, group II, positive control, were challenged with pathogenic S. aureus, group III mice were challenged and treated with AX, and group IV were treated with amoxicillin plus sulbactum. The TCS was higher in postchallenged mice; however it was significantly higher in group II untreated mice as compared to group III and group IV mice. The neutrophil was higher and lymphocyte count was lower in group II mice at 120 hrs post challenge (PC). The CRP was positive in all the challenged group at 24 hrs PC, but it remained positive till 120 hrs PC in group II. The parameters are related to enhancement of the mammary defense and reduction of inflammation. Hence AX may be used alone or as an adjunct therapy with antibiotic for amelioration of mastitis. Development of such therapy may be useful to reduce the antibiotic burden in management of intramammary infection.

No MeSH data available.


Related in: MedlinePlus