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Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation.

Morrell JM, Johannisson A - J Vet Med (2014)

Bottom Line: The proportion of spermatozoa with high velocity was increased compared to untreated SLC-selected spermatozoa (P < 0.05), with significant differences between individuals (P < 0.01).Whether homologous SP or heterologous SP had a greater effect on progressive motility depended on the individual.If SP is to be added to take advantage of a transient increase in progressive motility for a particular individual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect.

View Article: PubMed Central - PubMed

Affiliation: Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden.

ABSTRACT
The effect on sperm motility and chromatin integrity of adding homologous or heterologous equine seminal plasma (SP) to fresh stallion spermatozoa selected by single layer centrifugation (SLC) was studied. No statistical difference in mean progressive motility was seen after adding SP at time 0 h, although there were differences for individual stallions. The proportion of spermatozoa with high velocity was increased compared to untreated SLC-selected spermatozoa (P < 0.05), with significant differences between individuals (P < 0.01). When the SLC samples were stored for 24 h before adding SP, a significant increase in mean progressive motility was seen for SLC + homologous SP (P < 0.01) and for SLC + heterologous SP (P < 0.056). Whether homologous SP or heterologous SP had a greater effect on progressive motility depended on the individual. Adding either type of SP caused a significant increase in chromatin damage compared to SLC after storage for 24 h (homologous SP, P < 0.05; heterologous SP, P < 0.01). These preliminary data showed that storage of SLC-spermatozoa mixed with SP should be avoided because of the risk of increased chromatin damage. If SP is to be added to take advantage of a transient increase in progressive motility for a particular individual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect.

No MeSH data available.


Related in: MedlinePlus

Effect of adding foreign seminal plasma, own seminal plasma, or no seminal plasma to sperm samples prepared by single layer centrifugation on sperm motility; (a) semnal plasma added at 0 h after SLC; (b) 24 h after addition of seminal plasma; (c) seminal plasma added at 24 h after SLC. Note: black bars = foreign seminal plasma, white bars = own seminal plasma, and dotted bars = no seminal plasma.
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Related In: Results  -  Collection


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fig1: Effect of adding foreign seminal plasma, own seminal plasma, or no seminal plasma to sperm samples prepared by single layer centrifugation on sperm motility; (a) semnal plasma added at 0 h after SLC; (b) 24 h after addition of seminal plasma; (c) seminal plasma added at 24 h after SLC. Note: black bars = foreign seminal plasma, white bars = own seminal plasma, and dotted bars = no seminal plasma.

Mentions: There was considerable variation between stallions in the response to addition of pooled heterologous SP to fresh SLC-selected samples (Figure 1). For three stallions, PM was the highest in the SLC samples than in SP-treated samples. For six of the 11 stallions, heterologous SP induced a greater increase in PM than homologous SP, but for the remaining five stallions homologous SP caused a greater increase in PM than heterologous SP. In the treated samples after 24 h cold storage, PM was again the highest for pooled heterologous SP for five stallions. However, in the remaining stallions, the highest PM was seen in SLC samples for three stallions and for homologous SP for the other three stallions, although the ranking was not the same as at 0 h. Addition of SP to SLC-samples that had been stored for 24 h after SLC caused a significant increase in PM for ten stallions and a decrease in PM in one stallion (stallion H). Homologous SP caused a greater increase in PM than pooled heterologous SP in seven ejaculates.


Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation.

Morrell JM, Johannisson A - J Vet Med (2014)

Effect of adding foreign seminal plasma, own seminal plasma, or no seminal plasma to sperm samples prepared by single layer centrifugation on sperm motility; (a) semnal plasma added at 0 h after SLC; (b) 24 h after addition of seminal plasma; (c) seminal plasma added at 24 h after SLC. Note: black bars = foreign seminal plasma, white bars = own seminal plasma, and dotted bars = no seminal plasma.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590871&req=5

fig1: Effect of adding foreign seminal plasma, own seminal plasma, or no seminal plasma to sperm samples prepared by single layer centrifugation on sperm motility; (a) semnal plasma added at 0 h after SLC; (b) 24 h after addition of seminal plasma; (c) seminal plasma added at 24 h after SLC. Note: black bars = foreign seminal plasma, white bars = own seminal plasma, and dotted bars = no seminal plasma.
Mentions: There was considerable variation between stallions in the response to addition of pooled heterologous SP to fresh SLC-selected samples (Figure 1). For three stallions, PM was the highest in the SLC samples than in SP-treated samples. For six of the 11 stallions, heterologous SP induced a greater increase in PM than homologous SP, but for the remaining five stallions homologous SP caused a greater increase in PM than heterologous SP. In the treated samples after 24 h cold storage, PM was again the highest for pooled heterologous SP for five stallions. However, in the remaining stallions, the highest PM was seen in SLC samples for three stallions and for homologous SP for the other three stallions, although the ranking was not the same as at 0 h. Addition of SP to SLC-samples that had been stored for 24 h after SLC caused a significant increase in PM for ten stallions and a decrease in PM in one stallion (stallion H). Homologous SP caused a greater increase in PM than pooled heterologous SP in seven ejaculates.

Bottom Line: The proportion of spermatozoa with high velocity was increased compared to untreated SLC-selected spermatozoa (P < 0.05), with significant differences between individuals (P < 0.01).Whether homologous SP or heterologous SP had a greater effect on progressive motility depended on the individual.If SP is to be added to take advantage of a transient increase in progressive motility for a particular individual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect.

View Article: PubMed Central - PubMed

Affiliation: Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden.

ABSTRACT
The effect on sperm motility and chromatin integrity of adding homologous or heterologous equine seminal plasma (SP) to fresh stallion spermatozoa selected by single layer centrifugation (SLC) was studied. No statistical difference in mean progressive motility was seen after adding SP at time 0 h, although there were differences for individual stallions. The proportion of spermatozoa with high velocity was increased compared to untreated SLC-selected spermatozoa (P < 0.05), with significant differences between individuals (P < 0.01). When the SLC samples were stored for 24 h before adding SP, a significant increase in mean progressive motility was seen for SLC + homologous SP (P < 0.01) and for SLC + heterologous SP (P < 0.056). Whether homologous SP or heterologous SP had a greater effect on progressive motility depended on the individual. Adding either type of SP caused a significant increase in chromatin damage compared to SLC after storage for 24 h (homologous SP, P < 0.05; heterologous SP, P < 0.01). These preliminary data showed that storage of SLC-spermatozoa mixed with SP should be avoided because of the risk of increased chromatin damage. If SP is to be added to take advantage of a transient increase in progressive motility for a particular individual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect.

No MeSH data available.


Related in: MedlinePlus