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A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations.

Valentina M, Grazia G, Pierre M, Gloria B, Ilaria L - J Vet Med (2013)

Bottom Line: IOX concentrations were determined by using a new HPLC-UV method.Correlation and agreement analysis between the GFR values obtained by a seven-point clearance method and the GFR values determined by the application of simplified sample combinations indicated that the 3-blood sample clearance model (5, 30, and 60 min) was the best simplified method because it provided an accurate GFR value in only one hour.The reported method is a simple and accurate way of GFR determination, which may be easily used in a clinical setting.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinics, University of Pisa, San Piero a Grado, Via Livornese Lato Monte, 56122 Pisa, Italy.

ABSTRACT
Glomerular filtration rate (GFR) is still a highly underutilized tool in cats because available methods are not easy to be performed in clinical practice. Iohexol (IOX) has been shown to be a useful and reliable marker of GFR both in animals and in humans. The aim of the present study was to develop a rapid and reliable method for measuring IOX in feline plasma and to evaluate the accuracy of limited sampling models to establish a low-cost and clinically suitable GFR test. IOX concentrations were determined by using a new HPLC-UV method. GFR was assessed as plasma clearance of IOX, which was calculated by dividing dose administered by area under the curve of plasmatic concentration versus time (AUC), and indexed to body weight (BW). Correlation and agreement analysis between the GFR values obtained by a seven-point clearance method and the GFR values determined by the application of simplified sample combinations indicated that the 3-blood sample clearance model (5, 30, and 60 min) was the best simplified method because it provided an accurate GFR value in only one hour. The reported method is a simple and accurate way of GFR determination, which may be easily used in a clinical setting.

No MeSH data available.


Bland-Altman plots of difference versus average of GFR between the 7-point reference clearance and simplified clearance models (a), (b), (c), and (d) in nonazotaemic cats (n = 35).
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fig4: Bland-Altman plots of difference versus average of GFR between the 7-point reference clearance and simplified clearance models (a), (b), (c), and (d) in nonazotaemic cats (n = 35).

Mentions: Pearson correlation testing between GFR values obtained by reference method and Model A (5, 30, 60, and 240 mins) showed a positive linear correlation (R2 = 0.92, P = 0.95; Figure 3(a)). Pearson correlation testing between GFR values obtained by reference method and Model B (5, 30, 60, and 120 mins) showed a positive linear correlation (R2 = 0.95, P = 0.97; Figure 3(b)). Pearson correlation testing between GFR values obtained by reference method and Model C (5, 60, 120, and 240 mins) showed a positive linear correlation (R2 = 0.88, P = 0.94; Figure 3(c)). Pearson correlation testing between GFR values obtained by reference method and Model D (5, 30, and 60 mins) showed a positive linear correlation (R2 = 0.83, P = 0.91; Figure 3(d)). The results from Bland-Altman analysis are given in Figure 4. The accuracies of all models tested were not significantly different from those of the reference method (Table 3) .


A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations.

Valentina M, Grazia G, Pierre M, Gloria B, Ilaria L - J Vet Med (2013)

Bland-Altman plots of difference versus average of GFR between the 7-point reference clearance and simplified clearance models (a), (b), (c), and (d) in nonazotaemic cats (n = 35).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590869&req=5

fig4: Bland-Altman plots of difference versus average of GFR between the 7-point reference clearance and simplified clearance models (a), (b), (c), and (d) in nonazotaemic cats (n = 35).
Mentions: Pearson correlation testing between GFR values obtained by reference method and Model A (5, 30, 60, and 240 mins) showed a positive linear correlation (R2 = 0.92, P = 0.95; Figure 3(a)). Pearson correlation testing between GFR values obtained by reference method and Model B (5, 30, 60, and 120 mins) showed a positive linear correlation (R2 = 0.95, P = 0.97; Figure 3(b)). Pearson correlation testing between GFR values obtained by reference method and Model C (5, 60, 120, and 240 mins) showed a positive linear correlation (R2 = 0.88, P = 0.94; Figure 3(c)). Pearson correlation testing between GFR values obtained by reference method and Model D (5, 30, and 60 mins) showed a positive linear correlation (R2 = 0.83, P = 0.91; Figure 3(d)). The results from Bland-Altman analysis are given in Figure 4. The accuracies of all models tested were not significantly different from those of the reference method (Table 3) .

Bottom Line: IOX concentrations were determined by using a new HPLC-UV method.Correlation and agreement analysis between the GFR values obtained by a seven-point clearance method and the GFR values determined by the application of simplified sample combinations indicated that the 3-blood sample clearance model (5, 30, and 60 min) was the best simplified method because it provided an accurate GFR value in only one hour.The reported method is a simple and accurate way of GFR determination, which may be easily used in a clinical setting.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinics, University of Pisa, San Piero a Grado, Via Livornese Lato Monte, 56122 Pisa, Italy.

ABSTRACT
Glomerular filtration rate (GFR) is still a highly underutilized tool in cats because available methods are not easy to be performed in clinical practice. Iohexol (IOX) has been shown to be a useful and reliable marker of GFR both in animals and in humans. The aim of the present study was to develop a rapid and reliable method for measuring IOX in feline plasma and to evaluate the accuracy of limited sampling models to establish a low-cost and clinically suitable GFR test. IOX concentrations were determined by using a new HPLC-UV method. GFR was assessed as plasma clearance of IOX, which was calculated by dividing dose administered by area under the curve of plasmatic concentration versus time (AUC), and indexed to body weight (BW). Correlation and agreement analysis between the GFR values obtained by a seven-point clearance method and the GFR values determined by the application of simplified sample combinations indicated that the 3-blood sample clearance model (5, 30, and 60 min) was the best simplified method because it provided an accurate GFR value in only one hour. The reported method is a simple and accurate way of GFR determination, which may be easily used in a clinical setting.

No MeSH data available.