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Effects of Experimental Sarcocystis neurona-Induced Infection on Immunity in an Equine Model.

Lewis SR, Ellison SP, Dascanio JJ, Lindsay DS, Gogal RM, Werre SR, Surendran N, Breen ME, Heid BM, Andrews FM, Buechner-Maxwell VA, Witonsky SG - J Vet Med (2014)

Bottom Line: Neurologic parameters were recorded prior to and throughout the 70-day study by blinded investigators.Recombinant SnSAG1 ELISA for serum and CSF were used to confirm and track disease progression.Neutrophils, monocytes, and lymphocytes from infected horses displayed significantly delayed apoptosis at some time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061, USA ; Rangiora Veterinary Centre, Rangiora 7400, New Zealand.

ABSTRACT
Sarcocystis neurona is the most common cause of Equine Protozoal Myeloencephalitis (EPM), affecting 0.5-1% horses in the United States during their lifetimes. The objective of this study was to evaluate the equine immune responses in an experimentally induced Sarcocystis neurona infection model. Neurologic parameters were recorded prior to and throughout the 70-day study by blinded investigators. Recombinant SnSAG1 ELISA for serum and CSF were used to confirm and track disease progression. All experimentally infected horses displayed neurologic signs after infection. Neutrophils, monocytes, and lymphocytes from infected horses displayed significantly delayed apoptosis at some time points. Cell proliferation was significantly increased in S. neurona-infected horses when stimulated nonspecifically with PMA/I but significantly decreased when stimulated with S. neurona compared to controls. Collectively, our results suggest that horses experimentally infected with S. neurona manifest impaired antigen specific response to S. neurona, which could be a function of altered antigen presentation, lack of antigen recognition, or both.

No MeSH data available.


Related in: MedlinePlus

Gating for apoptosis data. 2a: gate of live versus dying lymphocytes after (a) 24 hrs, (b) 48 hrs, and (c) 72 hrs. (d) A sample of the 7-AAD gating of viable, early, and late apoptotic cells.
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fig6: Gating for apoptosis data. 2a: gate of live versus dying lymphocytes after (a) 24 hrs, (b) 48 hrs, and (c) 72 hrs. (d) A sample of the 7-AAD gating of viable, early, and late apoptotic cells.

Mentions: For cells cultured with media only, PMA/I, and merozoite stimulated samples, viable, early, and late apoptotic populations were defined based on 7-AAD staining (Figure 6). All samples were analyzed using the same electronic gates. Most data are presented in the context of significant differences between the experimentally infected versus non-experimentally infected control animals (Table 5).


Effects of Experimental Sarcocystis neurona-Induced Infection on Immunity in an Equine Model.

Lewis SR, Ellison SP, Dascanio JJ, Lindsay DS, Gogal RM, Werre SR, Surendran N, Breen ME, Heid BM, Andrews FM, Buechner-Maxwell VA, Witonsky SG - J Vet Med (2014)

Gating for apoptosis data. 2a: gate of live versus dying lymphocytes after (a) 24 hrs, (b) 48 hrs, and (c) 72 hrs. (d) A sample of the 7-AAD gating of viable, early, and late apoptotic cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590861&req=5

fig6: Gating for apoptosis data. 2a: gate of live versus dying lymphocytes after (a) 24 hrs, (b) 48 hrs, and (c) 72 hrs. (d) A sample of the 7-AAD gating of viable, early, and late apoptotic cells.
Mentions: For cells cultured with media only, PMA/I, and merozoite stimulated samples, viable, early, and late apoptotic populations were defined based on 7-AAD staining (Figure 6). All samples were analyzed using the same electronic gates. Most data are presented in the context of significant differences between the experimentally infected versus non-experimentally infected control animals (Table 5).

Bottom Line: Neurologic parameters were recorded prior to and throughout the 70-day study by blinded investigators.Recombinant SnSAG1 ELISA for serum and CSF were used to confirm and track disease progression.Neutrophils, monocytes, and lymphocytes from infected horses displayed significantly delayed apoptosis at some time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061, USA ; Rangiora Veterinary Centre, Rangiora 7400, New Zealand.

ABSTRACT
Sarcocystis neurona is the most common cause of Equine Protozoal Myeloencephalitis (EPM), affecting 0.5-1% horses in the United States during their lifetimes. The objective of this study was to evaluate the equine immune responses in an experimentally induced Sarcocystis neurona infection model. Neurologic parameters were recorded prior to and throughout the 70-day study by blinded investigators. Recombinant SnSAG1 ELISA for serum and CSF were used to confirm and track disease progression. All experimentally infected horses displayed neurologic signs after infection. Neutrophils, monocytes, and lymphocytes from infected horses displayed significantly delayed apoptosis at some time points. Cell proliferation was significantly increased in S. neurona-infected horses when stimulated nonspecifically with PMA/I but significantly decreased when stimulated with S. neurona compared to controls. Collectively, our results suggest that horses experimentally infected with S. neurona manifest impaired antigen specific response to S. neurona, which could be a function of altered antigen presentation, lack of antigen recognition, or both.

No MeSH data available.


Related in: MedlinePlus