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Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Sammra O, Balbutskaya A, Hijazin M, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Prenger-Berninghoff E, Timke M, Kostrzewa M, Siebert U - J Vet Med (2014)

Bottom Line: Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap.The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear.However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

View Article: PubMed Central - PubMed

Affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.

ABSTRACT
Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

No MeSH data available.


Related in: MedlinePlus

Dendrogram analysis of 16S rDNA sequences of the A. phocisimile strains of the present study and reference strains of genus Arcanobacterium obtained from NCBI GenBank.
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Related In: Results  -  Collection


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fig2: Dendrogram analysis of 16S rDNA sequences of the A. phocisimile strains of the present study and reference strains of genus Arcanobacterium obtained from NCBI GenBank.

Mentions: The genotypic classification by 16S rDNA sequencing revealed that the three novel A. phocisimile strains of the present study yielded 100% identity to both A. phocisimile strains described previously [3], also including the type strain A. phocisimile  2698T (Figure 2).


Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Sammra O, Balbutskaya A, Hijazin M, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Prenger-Berninghoff E, Timke M, Kostrzewa M, Siebert U - J Vet Med (2014)

Dendrogram analysis of 16S rDNA sequences of the A. phocisimile strains of the present study and reference strains of genus Arcanobacterium obtained from NCBI GenBank.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590858&req=5

fig2: Dendrogram analysis of 16S rDNA sequences of the A. phocisimile strains of the present study and reference strains of genus Arcanobacterium obtained from NCBI GenBank.
Mentions: The genotypic classification by 16S rDNA sequencing revealed that the three novel A. phocisimile strains of the present study yielded 100% identity to both A. phocisimile strains described previously [3], also including the type strain A. phocisimile  2698T (Figure 2).

Bottom Line: Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap.The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear.However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

View Article: PubMed Central - PubMed

Affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.

ABSTRACT
Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

No MeSH data available.


Related in: MedlinePlus