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Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Sammra O, Balbutskaya A, Hijazin M, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Prenger-Berninghoff E, Timke M, Kostrzewa M, Siebert U - J Vet Med (2014)

Bottom Line: Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap.The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear.However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

View Article: PubMed Central - PubMed

Affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.

ABSTRACT
Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

No MeSH data available.


Related in: MedlinePlus

MALDI-TOF MS spectra from A. phocisimile 4113, A. phocisimile 4125, type strain A. phocisimile  2698T, A. phocisimile 4112, A. phocisimile 3047, and all other species of genus Arcanobacterium.
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fig1: MALDI-TOF MS spectra from A. phocisimile 4113, A. phocisimile 4125, type strain A. phocisimile  2698T, A. phocisimile 4112, A. phocisimile 3047, and all other species of genus Arcanobacterium.

Mentions: As shown by numerous authors MALDI-TOF MS is a powerful tool for species characterization of a broad spectrum of gram-positive and gram-negative bacteria [8–10]. This technique had previously been successfully used for rapid and reliable identification of bacteria of genera Arcanobacterium and Trueperella [5, 11]. The MALDI-TOF MS analysis of the present study revealed that by using the current Bruker data base, all five strains of this hitherto unknown species could not be identified to species level. However, using the MALDI Biotyper 3.1 software package the log (score) values of A. phocisimile 4112, A. phocisimile 3047, A. phocisimile 4113, and A. phocisimile 4125 matched against A. phocisimile  2698T with log (score) values between 2.69 and 2.74 indicating that all five strains belong to this newly described species. Inclusion of A. phocisimile in the Bruker reference database will allow for the identification of this new species in future. A dendrogram analysis of the MALDI-TOF MS results is presented in Figure 1.


Further Studies on Arcanobacterium phocisimile: a Novel Species of Genus Arcanobacterium.

Sammra O, Balbutskaya A, Hijazin M, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Prenger-Berninghoff E, Timke M, Kostrzewa M, Siebert U - J Vet Med (2014)

MALDI-TOF MS spectra from A. phocisimile 4113, A. phocisimile 4125, type strain A. phocisimile  2698T, A. phocisimile 4112, A. phocisimile 3047, and all other species of genus Arcanobacterium.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590858&req=5

fig1: MALDI-TOF MS spectra from A. phocisimile 4113, A. phocisimile 4125, type strain A. phocisimile  2698T, A. phocisimile 4112, A. phocisimile 3047, and all other species of genus Arcanobacterium.
Mentions: As shown by numerous authors MALDI-TOF MS is a powerful tool for species characterization of a broad spectrum of gram-positive and gram-negative bacteria [8–10]. This technique had previously been successfully used for rapid and reliable identification of bacteria of genera Arcanobacterium and Trueperella [5, 11]. The MALDI-TOF MS analysis of the present study revealed that by using the current Bruker data base, all five strains of this hitherto unknown species could not be identified to species level. However, using the MALDI Biotyper 3.1 software package the log (score) values of A. phocisimile 4112, A. phocisimile 3047, A. phocisimile 4113, and A. phocisimile 4125 matched against A. phocisimile  2698T with log (score) values between 2.69 and 2.74 indicating that all five strains belong to this newly described species. Inclusion of A. phocisimile in the Bruker reference database will allow for the identification of this new species in future. A dendrogram analysis of the MALDI-TOF MS results is presented in Figure 1.

Bottom Line: Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap.The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear.However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

View Article: PubMed Central - PubMed

Affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, 35392 Gießen, Germany.

ABSTRACT
Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.

No MeSH data available.


Related in: MedlinePlus