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Sternal Aspiration of Bone Marrow in Dogs: A Practical Approach for Canine Leishmaniasis Diagnosis and Monitoring.

Paparcone R, Fiorentino E, Cappiello S, Gizzarelli M, Gradoni L, Oliva G, Foglia Manzillo V - J Vet Med (2013)

Bottom Line: Animals are positioned in right lateral recumbency, and the area corresponding to the second, third, or fourth sternebra is identified and aseptically prepared.A 18-gauge needle connected to a 10 mL syringe is driven through the skin, up to the bone wall, and firmly pushed forward while rotating.Entry into the sternebra's cavity is clearly perceived by the fall of resistance offered by the cortex.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, 80137 Naples, Italy.

ABSTRACT
Bone-marrow aspirate material is commonly considered as one of the most sensitive tissues for a reliable diagnosis of leishmaniasis. The procedure herein described may permit less experienced veterinarians to be familiar with a quick and safe assessment method for leishmaniasis diagnosis in their patients. Animals are positioned in right lateral recumbency, and the area corresponding to the second, third, or fourth sternebra is identified and aseptically prepared. A 18-gauge needle connected to a 10 mL syringe is driven through the skin, up to the bone wall, and firmly pushed forward while rotating. Entry into the sternebra's cavity is clearly perceived by the fall of resistance offered by the cortex. Some 2,500 sternal bone-marrow samplings were safely and efficiently performed on 887 dogs of different breeds and aging from 6 months to 14 years, during eight years of clinical activity for routine diagnosis of canine leishmaniasis in pets or for the efficacy evaluation of anti-Leishmania immunobiologicals in dogs naturally exposed to parasite transmission. Most of the samples (1716) were from 387 dogs enrolled for anti-Leishmania vaccine studies. The safety of the method was particularly assessed on these dogs that as per study protocol were submitted to repeated bone-marrow aspirations (2-4 per year) in follow-up examinations.

No MeSH data available.


Related in: MedlinePlus

Anatomical site for sternal aspiration.
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Related In: Results  -  Collection


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fig1: Anatomical site for sternal aspiration.

Mentions: Briefly, the animals were positioned in right lateral recumbency with the right foreleg kept forward and the left foreleg kept backward, both parallel with the body axis. The area corresponding to the second, third, or fourth sternebra (Figure 1) was identified by palpation, clipped, and aseptically prepared. The needle was threaded through the skin up to the sternebra's wall and firmly pushed forward, while rotating (Figure 2). Entry into the bone cavity is clearly perceived thanks to the fall in resistance offered by the bone cortex. Approximately 5 mL of vacuum was then applied for 5 to 10 seconds depending on the volume of bone-marrow material required, usually around 0.5 mL. In some cases the aspiration of material may fail at the first attempt due to the incorrect insertion of the needle or to the needle obstruction by bone splinters. In these circumstances, it is sufficient to repeat quickly the procedure after changing the needle. This inconvenience occurred in about 5% of dogs.


Sternal Aspiration of Bone Marrow in Dogs: A Practical Approach for Canine Leishmaniasis Diagnosis and Monitoring.

Paparcone R, Fiorentino E, Cappiello S, Gizzarelli M, Gradoni L, Oliva G, Foglia Manzillo V - J Vet Med (2013)

Anatomical site for sternal aspiration.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590857&req=5

fig1: Anatomical site for sternal aspiration.
Mentions: Briefly, the animals were positioned in right lateral recumbency with the right foreleg kept forward and the left foreleg kept backward, both parallel with the body axis. The area corresponding to the second, third, or fourth sternebra (Figure 1) was identified by palpation, clipped, and aseptically prepared. The needle was threaded through the skin up to the sternebra's wall and firmly pushed forward, while rotating (Figure 2). Entry into the bone cavity is clearly perceived thanks to the fall in resistance offered by the bone cortex. Approximately 5 mL of vacuum was then applied for 5 to 10 seconds depending on the volume of bone-marrow material required, usually around 0.5 mL. In some cases the aspiration of material may fail at the first attempt due to the incorrect insertion of the needle or to the needle obstruction by bone splinters. In these circumstances, it is sufficient to repeat quickly the procedure after changing the needle. This inconvenience occurred in about 5% of dogs.

Bottom Line: Animals are positioned in right lateral recumbency, and the area corresponding to the second, third, or fourth sternebra is identified and aseptically prepared.A 18-gauge needle connected to a 10 mL syringe is driven through the skin, up to the bone wall, and firmly pushed forward while rotating.Entry into the sternebra's cavity is clearly perceived by the fall of resistance offered by the cortex.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, 80137 Naples, Italy.

ABSTRACT
Bone-marrow aspirate material is commonly considered as one of the most sensitive tissues for a reliable diagnosis of leishmaniasis. The procedure herein described may permit less experienced veterinarians to be familiar with a quick and safe assessment method for leishmaniasis diagnosis in their patients. Animals are positioned in right lateral recumbency, and the area corresponding to the second, third, or fourth sternebra is identified and aseptically prepared. A 18-gauge needle connected to a 10 mL syringe is driven through the skin, up to the bone wall, and firmly pushed forward while rotating. Entry into the sternebra's cavity is clearly perceived by the fall of resistance offered by the cortex. Some 2,500 sternal bone-marrow samplings were safely and efficiently performed on 887 dogs of different breeds and aging from 6 months to 14 years, during eight years of clinical activity for routine diagnosis of canine leishmaniasis in pets or for the efficacy evaluation of anti-Leishmania immunobiologicals in dogs naturally exposed to parasite transmission. Most of the samples (1716) were from 387 dogs enrolled for anti-Leishmania vaccine studies. The safety of the method was particularly assessed on these dogs that as per study protocol were submitted to repeated bone-marrow aspirations (2-4 per year) in follow-up examinations.

No MeSH data available.


Related in: MedlinePlus