Limits...
Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan.

Khalifa KA, Sidahmed Abdelrahim E, Badwi M, Mohamed AM - J Vet Med (2013)

Bottom Line: Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests.The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively.This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Research Institute (VRI), P.O. Box 806, Khartoum, Sudan.

ABSTRACT
The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan. A number of 45 Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan. Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests. The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively. This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination. PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens.

No MeSH data available.


Related in: MedlinePlus

Electorophoresis profile of Ms DNA obtained from 3 samples by PCR (740 bp) on 2% Agarose. Lane 1, DNA Ladder; Lane 2: isolate F; Lane 3: J; Lane 4: K; Lane 5, positive control; Lane 6: negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4590853&req=5

fig3: Electorophoresis profile of Ms DNA obtained from 3 samples by PCR (740 bp) on 2% Agarose. Lane 1, DNA Ladder; Lane 2: isolate F; Lane 3: J; Lane 4: K; Lane 5, positive control; Lane 6: negative control.

Mentions: The three remaining isolates (F, J and K) representing 4 swabs (6.7%) were found positive to Ms (Table 2) as 720 bp products were obtained similar to the positive control of the kit, (Figure 3).


Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan.

Khalifa KA, Sidahmed Abdelrahim E, Badwi M, Mohamed AM - J Vet Med (2013)

Electorophoresis profile of Ms DNA obtained from 3 samples by PCR (740 bp) on 2% Agarose. Lane 1, DNA Ladder; Lane 2: isolate F; Lane 3: J; Lane 4: K; Lane 5, positive control; Lane 6: negative control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590853&req=5

fig3: Electorophoresis profile of Ms DNA obtained from 3 samples by PCR (740 bp) on 2% Agarose. Lane 1, DNA Ladder; Lane 2: isolate F; Lane 3: J; Lane 4: K; Lane 5, positive control; Lane 6: negative control.
Mentions: The three remaining isolates (F, J and K) representing 4 swabs (6.7%) were found positive to Ms (Table 2) as 720 bp products were obtained similar to the positive control of the kit, (Figure 3).

Bottom Line: Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests.The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively.This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Research Institute (VRI), P.O. Box 806, Khartoum, Sudan.

ABSTRACT
The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan. A number of 45 Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan. Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests. The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively. This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination. PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens.

No MeSH data available.


Related in: MedlinePlus