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Morphometric Identification, Gross and Histopathological Lesions of Eimeria Species in Japanese Quails (Coturnix coturnix japonica) in Zaria, Nigeria.

Umar HA, Lawal IA, Okubanjo OO, Wakawa AM - J Vet Med (2014)

Bottom Line: The main gross lesion seen was nonhaemorrhagic ballooning of the caeca.Intestinal scrapping smear revealed a developmental stage of the parasite (merozoites) in the jejunum.Further study is required using molecular techniques to properly identify the unknown species of Eimeria that were detected in the study.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria 2222, Nigeria.

ABSTRACT
The objective of the study was to identify the species, gross and histopathological lesions of Eimeria in Japanese quails in Zaria. A total of 400 fresh faecal samples were collected and 10 quail birds were purchased from a quail farm. The faecal samples were processed using simple floatation technique. Oocysts shape indices of sporulated oocysts were determined. The intestines were observed for gross lesions and segments were analyzed using Giemsa stain and Haematoxylin and Eosin stain and then observed microscopically for the developmental stages of the parasite. Four species of Eimeria were identified in the study. Eimeria bateri of shape index of 1.36 conformed to the guidelines used while the other three Eimeria species with shape indices of 1.48, 1.03, and 1.40 were not confirmed. The main gross lesion seen was nonhaemorrhagic ballooning of the caeca. Intestinal scrapping smear revealed a developmental stage of the parasite (merozoites) in the jejunum. Histopathology also revealed a developmental stage (schizont) of the parasite in the caecum and desquamation of the epithelial lining with areas of necrosis. Further study is required using molecular techniques to properly identify the unknown species of Eimeria that were detected in the study.

No MeSH data available.


Related in: MedlinePlus

Jejunum of Japanese quail showing the developmental stage (merozoite) of Eimeria spp. in the epithelium using Giemsa stain (×100) oil immersion.
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Related In: Results  -  Collection


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fig7: Jejunum of Japanese quail showing the developmental stage (merozoite) of Eimeria spp. in the epithelium using Giemsa stain (×100) oil immersion.

Mentions: The fresh faecal sample was positive for Eimeria oocysts (Figure 1). The main gross pathology seen was the ballooning of the caeca which contains no bloody exudate in the lumen (Figure 6). Histopathologically, desquamation of intestinal mucosa was observed (Figure 9) and caecal necrosis (Figure 8). Developmental stages of the parasite especially the merozoites and schizont were seen in the intestinal epithelium (Figures 7, 8, and 9).


Morphometric Identification, Gross and Histopathological Lesions of Eimeria Species in Japanese Quails (Coturnix coturnix japonica) in Zaria, Nigeria.

Umar HA, Lawal IA, Okubanjo OO, Wakawa AM - J Vet Med (2014)

Jejunum of Japanese quail showing the developmental stage (merozoite) of Eimeria spp. in the epithelium using Giemsa stain (×100) oil immersion.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590851&req=5

fig7: Jejunum of Japanese quail showing the developmental stage (merozoite) of Eimeria spp. in the epithelium using Giemsa stain (×100) oil immersion.
Mentions: The fresh faecal sample was positive for Eimeria oocysts (Figure 1). The main gross pathology seen was the ballooning of the caeca which contains no bloody exudate in the lumen (Figure 6). Histopathologically, desquamation of intestinal mucosa was observed (Figure 9) and caecal necrosis (Figure 8). Developmental stages of the parasite especially the merozoites and schizont were seen in the intestinal epithelium (Figures 7, 8, and 9).

Bottom Line: The main gross lesion seen was nonhaemorrhagic ballooning of the caeca.Intestinal scrapping smear revealed a developmental stage of the parasite (merozoites) in the jejunum.Further study is required using molecular techniques to properly identify the unknown species of Eimeria that were detected in the study.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria 2222, Nigeria.

ABSTRACT
The objective of the study was to identify the species, gross and histopathological lesions of Eimeria in Japanese quails in Zaria. A total of 400 fresh faecal samples were collected and 10 quail birds were purchased from a quail farm. The faecal samples were processed using simple floatation technique. Oocysts shape indices of sporulated oocysts were determined. The intestines were observed for gross lesions and segments were analyzed using Giemsa stain and Haematoxylin and Eosin stain and then observed microscopically for the developmental stages of the parasite. Four species of Eimeria were identified in the study. Eimeria bateri of shape index of 1.36 conformed to the guidelines used while the other three Eimeria species with shape indices of 1.48, 1.03, and 1.40 were not confirmed. The main gross lesion seen was nonhaemorrhagic ballooning of the caeca. Intestinal scrapping smear revealed a developmental stage of the parasite (merozoites) in the jejunum. Histopathology also revealed a developmental stage (schizont) of the parasite in the caecum and desquamation of the epithelial lining with areas of necrosis. Further study is required using molecular techniques to properly identify the unknown species of Eimeria that were detected in the study.

No MeSH data available.


Related in: MedlinePlus