Limits...
Serratiopeptidase Niosomal Gel with Potential in Topical Delivery.

Shinde UA, Kanojiya SS - J Pharm (Cairo) (2014)

Bottom Line: The entrapment efficiency was found to be influenced by the molar ratio of Span 40 : cholesterol and concentration of SRP in noisome.Furthermore ex vivo skin permeation revealed that there was fourfold increase in a steady state flux when SRP was formulated in niosomes and a significant increase in the permeation of SRP, from SRP niosomal gel containing permeation enhancer.In vivo efficacy studies indicated that SRP niosomal gel had a comparable topical anti-inflammatory activity to that of dicolfenac gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400098, India.

ABSTRACT
The objective of present study was to develop nonionic surfactant vesicles of proteolytic enzyme serratiopeptidase (SRP) by adapting reverse phase evaporation (REV) technique and to evaluate the viability of SRP niosomal gel in treating the topical inflammation. The feasibility of SRP niosomes by REV method using Span 40 and cholesterol has been successfully demonstrated in this investigation. The entrapment efficiency was found to be influenced by the molar ratio of Span 40 : cholesterol and concentration of SRP in noisome. The developed niosomes were characterized for morphology, particle size, and in vitro release. Niosomal gel was prepared by dispersing xanthan gum into optimized batch of SRP niosomes. Ex vivo permeation and in vivo anti-inflammatory efficacy of gel formulation were evaluated topically. SRP niosomes obtained were round in nanosize range. At Span 40 : cholesterol molar ratio 1 : 1 entrapment efficiency was maximum, that is, 54.82% ± 2.08, and showed consistent release pattern. Furthermore ex vivo skin permeation revealed that there was fourfold increase in a steady state flux when SRP was formulated in niosomes and a significant increase in the permeation of SRP, from SRP niosomal gel containing permeation enhancer. In vivo efficacy studies indicated that SRP niosomal gel had a comparable topical anti-inflammatory activity to that of dicolfenac gel.

No MeSH data available.


Related in: MedlinePlus

Rheology of SRP niosomal gel.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4590824&req=5

fig3: Rheology of SRP niosomal gel.

Mentions: It was noted that there was change in particle size when niosomes were formulated in gel form using xanthan gum. In niosomal gel the particle size was increased from 362.21 μ to 978.8 μ. Similar results were reported by Antunes et al. for diclofenac sodium niosomal Pluronic gel. The increase in particle in SRP niosomal gel could be due to the formation of polymer layer along the vesicle surface [19]. The low values of polydispersity index indicated that there was no aggregation of vesicles in the gel. The drug content of the SRP niosomal gels is as shown in Table 3 and was well within limits. There was no degradation of SRP in the niosomes when formulated as niosomal gels. The larger the area the better the spreadability and good ability to spread over larger surface area. Formulation gelled with xanthan gum showed better spreadability (area 31.15 ± 0.15 cm2), whereas the area for marketed diclofenac gel formulation was found to be 18.08 ± 0.28 cm2, indicating that the spreadability of SRP niosomal gels was better than that of marketed diclofenac gel. This could be because of the loose gel matrix nature of niosomal gel due to presence of vesicles. The pH range for skin is 6–8. As shown in Table 3, the pH values of all formulations were found to be compatible for topical application. From the rheogram curve (Figure 3) we could conclude that SRP niosomal gel formulation exhibited pseudoplastic flow behavior. The pseudoplastic (shear thinning) behavior of xanthan gum gel has been observed previously [13]. SRP niosomal gel had viscosity in the range of 3.8 poise to 3.3 poise.


Serratiopeptidase Niosomal Gel with Potential in Topical Delivery.

Shinde UA, Kanojiya SS - J Pharm (Cairo) (2014)

Rheology of SRP niosomal gel.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590824&req=5

fig3: Rheology of SRP niosomal gel.
Mentions: It was noted that there was change in particle size when niosomes were formulated in gel form using xanthan gum. In niosomal gel the particle size was increased from 362.21 μ to 978.8 μ. Similar results were reported by Antunes et al. for diclofenac sodium niosomal Pluronic gel. The increase in particle in SRP niosomal gel could be due to the formation of polymer layer along the vesicle surface [19]. The low values of polydispersity index indicated that there was no aggregation of vesicles in the gel. The drug content of the SRP niosomal gels is as shown in Table 3 and was well within limits. There was no degradation of SRP in the niosomes when formulated as niosomal gels. The larger the area the better the spreadability and good ability to spread over larger surface area. Formulation gelled with xanthan gum showed better spreadability (area 31.15 ± 0.15 cm2), whereas the area for marketed diclofenac gel formulation was found to be 18.08 ± 0.28 cm2, indicating that the spreadability of SRP niosomal gels was better than that of marketed diclofenac gel. This could be because of the loose gel matrix nature of niosomal gel due to presence of vesicles. The pH range for skin is 6–8. As shown in Table 3, the pH values of all formulations were found to be compatible for topical application. From the rheogram curve (Figure 3) we could conclude that SRP niosomal gel formulation exhibited pseudoplastic flow behavior. The pseudoplastic (shear thinning) behavior of xanthan gum gel has been observed previously [13]. SRP niosomal gel had viscosity in the range of 3.8 poise to 3.3 poise.

Bottom Line: The entrapment efficiency was found to be influenced by the molar ratio of Span 40 : cholesterol and concentration of SRP in noisome.Furthermore ex vivo skin permeation revealed that there was fourfold increase in a steady state flux when SRP was formulated in niosomes and a significant increase in the permeation of SRP, from SRP niosomal gel containing permeation enhancer.In vivo efficacy studies indicated that SRP niosomal gel had a comparable topical anti-inflammatory activity to that of dicolfenac gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (East), Mumbai 400098, India.

ABSTRACT
The objective of present study was to develop nonionic surfactant vesicles of proteolytic enzyme serratiopeptidase (SRP) by adapting reverse phase evaporation (REV) technique and to evaluate the viability of SRP niosomal gel in treating the topical inflammation. The feasibility of SRP niosomes by REV method using Span 40 and cholesterol has been successfully demonstrated in this investigation. The entrapment efficiency was found to be influenced by the molar ratio of Span 40 : cholesterol and concentration of SRP in noisome. The developed niosomes were characterized for morphology, particle size, and in vitro release. Niosomal gel was prepared by dispersing xanthan gum into optimized batch of SRP niosomes. Ex vivo permeation and in vivo anti-inflammatory efficacy of gel formulation were evaluated topically. SRP niosomes obtained were round in nanosize range. At Span 40 : cholesterol molar ratio 1 : 1 entrapment efficiency was maximum, that is, 54.82% ± 2.08, and showed consistent release pattern. Furthermore ex vivo skin permeation revealed that there was fourfold increase in a steady state flux when SRP was formulated in niosomes and a significant increase in the permeation of SRP, from SRP niosomal gel containing permeation enhancer. In vivo efficacy studies indicated that SRP niosomal gel had a comparable topical anti-inflammatory activity to that of dicolfenac gel.

No MeSH data available.


Related in: MedlinePlus