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Determination of Zalcitabine in Medicaments by Differential Pulse Voltammetry.

Leandro KC, Moreira JC, Farias PA - J Pharm (Cairo) (2013)

Bottom Line: The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables.The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L.The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

View Article: PubMed Central - PubMed

Affiliation: Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.

ABSTRACT
The zalcitabine (ddC) has been extensively used in the treatment of HIV patients due to its antiretroviral activity. The quality control of this active principle in medications is of outstanding importance to public health. The principal objective of the current study was the development of an alternative analytical methodology for the zalcitabine determination using a voltammetric process. The zalcitabine gives a reduction peak (at -1.22 V versus Ag/AgCl) at the hanging mercury drop electrode (HMDE). The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables. The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L. The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

No MeSH data available.


Related in: MedlinePlus

Mechanism of cytosine reduction [12].
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fig3: Mechanism of cytosine reduction [12].

Mentions: A new experiment was realized for a better comprehension of zalcitabine mechanism on the mercury surface. When an aqueous zalcitabine (10 mg · L−1) or cytosine solution is electrolyzed, the formation of ammonia could be observed in cases. The presence of ammonia was proved by Nessler's reagent forming a brown coloration or precipitate. These results and the similar structure of zalcitabine with that of cytosine are indicative that the reduction of the zalcitabine mechanism could be practically the same as proposed previously for cytosine (Figure 3).


Determination of Zalcitabine in Medicaments by Differential Pulse Voltammetry.

Leandro KC, Moreira JC, Farias PA - J Pharm (Cairo) (2013)

Mechanism of cytosine reduction [12].
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590814&req=5

fig3: Mechanism of cytosine reduction [12].
Mentions: A new experiment was realized for a better comprehension of zalcitabine mechanism on the mercury surface. When an aqueous zalcitabine (10 mg · L−1) or cytosine solution is electrolyzed, the formation of ammonia could be observed in cases. The presence of ammonia was proved by Nessler's reagent forming a brown coloration or precipitate. These results and the similar structure of zalcitabine with that of cytosine are indicative that the reduction of the zalcitabine mechanism could be practically the same as proposed previously for cytosine (Figure 3).

Bottom Line: The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables.The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L.The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

View Article: PubMed Central - PubMed

Affiliation: Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.

ABSTRACT
The zalcitabine (ddC) has been extensively used in the treatment of HIV patients due to its antiretroviral activity. The quality control of this active principle in medications is of outstanding importance to public health. The principal objective of the current study was the development of an alternative analytical methodology for the zalcitabine determination using a voltammetric process. The zalcitabine gives a reduction peak (at -1.22 V versus Ag/AgCl) at the hanging mercury drop electrode (HMDE). The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables. The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L. The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

No MeSH data available.


Related in: MedlinePlus