Limits...
Determination of Zalcitabine in Medicaments by Differential Pulse Voltammetry.

Leandro KC, Moreira JC, Farias PA - J Pharm (Cairo) (2013)

Bottom Line: The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables.The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L.The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

View Article: PubMed Central - PubMed

Affiliation: Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.

ABSTRACT
The zalcitabine (ddC) has been extensively used in the treatment of HIV patients due to its antiretroviral activity. The quality control of this active principle in medications is of outstanding importance to public health. The principal objective of the current study was the development of an alternative analytical methodology for the zalcitabine determination using a voltammetric process. The zalcitabine gives a reduction peak (at -1.22 V versus Ag/AgCl) at the hanging mercury drop electrode (HMDE). The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables. The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L. The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

No MeSH data available.


Related in: MedlinePlus

Cyclic voltammogram of the zalcitabine (20 mg · L−1) in 0.2 M Clark-Lubs buffer (pH 2.0). Scan rate: 100 mV s−1.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4590814&req=5

fig2: Cyclic voltammogram of the zalcitabine (20 mg · L−1) in 0.2 M Clark-Lubs buffer (pH 2.0). Scan rate: 100 mV s−1.

Mentions: Figure 2 shows a typical cyclic voltammogram for 20 mg · L−1 zalcitabine in a 0.2 M Clark-Lubs buffer solution (pH 2.0) using 100 mV/s as scan rate. The zalcitabine yields a well-defined reduction peak at −1.22 V during forward cathodic scan. No peaks are observed in the anodic branch indicating irreversibility of the electrodic reaction. Similar behavior was observed at pH below 5.5. In this range of pH no adsorption of zalcitabine in the mercury electrode surface was observed. Hence, the reduction peak at –1.22 V without accumulation was chosen for subsequent differential pulse voltammetric determinations, including the quantification of zalcitabine in antiretroviral drugs.


Determination of Zalcitabine in Medicaments by Differential Pulse Voltammetry.

Leandro KC, Moreira JC, Farias PA - J Pharm (Cairo) (2013)

Cyclic voltammogram of the zalcitabine (20 mg · L−1) in 0.2 M Clark-Lubs buffer (pH 2.0). Scan rate: 100 mV s−1.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590814&req=5

fig2: Cyclic voltammogram of the zalcitabine (20 mg · L−1) in 0.2 M Clark-Lubs buffer (pH 2.0). Scan rate: 100 mV s−1.
Mentions: Figure 2 shows a typical cyclic voltammogram for 20 mg · L−1 zalcitabine in a 0.2 M Clark-Lubs buffer solution (pH 2.0) using 100 mV/s as scan rate. The zalcitabine yields a well-defined reduction peak at −1.22 V during forward cathodic scan. No peaks are observed in the anodic branch indicating irreversibility of the electrodic reaction. Similar behavior was observed at pH below 5.5. In this range of pH no adsorption of zalcitabine in the mercury electrode surface was observed. Hence, the reduction peak at –1.22 V without accumulation was chosen for subsequent differential pulse voltammetric determinations, including the quantification of zalcitabine in antiretroviral drugs.

Bottom Line: The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables.The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L.The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

View Article: PubMed Central - PubMed

Affiliation: Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.

ABSTRACT
The zalcitabine (ddC) has been extensively used in the treatment of HIV patients due to its antiretroviral activity. The quality control of this active principle in medications is of outstanding importance to public health. The principal objective of the current study was the development of an alternative analytical methodology for the zalcitabine determination using a voltammetric process. The zalcitabine gives a reduction peak (at -1.22 V versus Ag/AgCl) at the hanging mercury drop electrode (HMDE). The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables. The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L. The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.

No MeSH data available.


Related in: MedlinePlus