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Plantago ovata F. Mucilage-Alginate Mucoadhesive Beads for Controlled Release of Glibenclamide: Development, Optimization, and In Vitro-In Vivo Evaluation.

Nayak AK, Pal D, Santra K - J Pharm (Cairo) (2013)

Bottom Line: The effects of sodium alginate (SA) to IHM and cross-linker (CaCl2) concentration on the drug encapsulation efficiency (DEE, %), as well as cumulative drug release after 10 hours (R10 h, %), were optimized using 3(2) factorial design based on response surface methodology.The in vitro drug release from these beads was followed by controlled release (zero-order) pattern with super case-II transport mechanism.The optimized glibenclamide-loaded IHM-alginate mucoadhesive beads showed significant antidiabetic effect in alloxan-induced diabetic rats over prolonged period after oral administration.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Seemanta Institute of Pharmaceutical Sciences, Jharpokharia, Mayurbhanj, Odisha 757086, India.

ABSTRACT
The current study deals with the development and optimization of ispaghula (Plantago ovata F.) husk mucilage- (IHM-) alginate mucoadhesive beads containing glibenclamide by ionotropic gelation technique. The effects of sodium alginate (SA) to IHM and cross-linker (CaCl2) concentration on the drug encapsulation efficiency (DEE, %), as well as cumulative drug release after 10 hours (R10 h, %), were optimized using 3(2) factorial design based on response surface methodology. The observed responses were coincided well with the predicted values by the experimental design. The optimized mucoadhesive beads exhibited 94.43 ± 4.80% w/w of DEE and good mucoadhesivity with the biological membrane in wash-off test and sustained drug release profile over 10 hours. The beads were also characterized by SEM and FTIR analyses. The in vitro drug release from these beads was followed by controlled release (zero-order) pattern with super case-II transport mechanism. The optimized glibenclamide-loaded IHM-alginate mucoadhesive beads showed significant antidiabetic effect in alloxan-induced diabetic rats over prolonged period after oral administration.

No MeSH data available.


Result of ex vivo wash-off test to assess mucoadhesive properties of optimized glibenclamide-loaded IHM-alginate beads in 0.1 N HCl, pH 1.2, and phosphate buffer, pH 7.4 (mean ± S.D., n = 3).
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fig14: Result of ex vivo wash-off test to assess mucoadhesive properties of optimized glibenclamide-loaded IHM-alginate beads in 0.1 N HCl, pH 1.2, and phosphate buffer, pH 7.4 (mean ± S.D., n = 3).

Mentions: The ex vivo wash-off behavior of optimized glibenclamide-loaded IHM-alginate beads (F-O) using goat intestinal mucosa was found faster in intestinal pH (7.4) than that in gastric pH (1.2). In gastric pH, the percentage of beads adhered onto the goat intestinal mucosal tissue varied from 64.88 ± 5.06% over 10 hours, whereas this was 30.47 ± 3.86% in intestinal pH (Figure 14). Thus, the results of the ex vivo wash-off test indicated that the newly developed optimized glibenclamide-loaded IHM-alginate beads had good mucoadhesivity.


Plantago ovata F. Mucilage-Alginate Mucoadhesive Beads for Controlled Release of Glibenclamide: Development, Optimization, and In Vitro-In Vivo Evaluation.

Nayak AK, Pal D, Santra K - J Pharm (Cairo) (2013)

Result of ex vivo wash-off test to assess mucoadhesive properties of optimized glibenclamide-loaded IHM-alginate beads in 0.1 N HCl, pH 1.2, and phosphate buffer, pH 7.4 (mean ± S.D., n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590812&req=5

fig14: Result of ex vivo wash-off test to assess mucoadhesive properties of optimized glibenclamide-loaded IHM-alginate beads in 0.1 N HCl, pH 1.2, and phosphate buffer, pH 7.4 (mean ± S.D., n = 3).
Mentions: The ex vivo wash-off behavior of optimized glibenclamide-loaded IHM-alginate beads (F-O) using goat intestinal mucosa was found faster in intestinal pH (7.4) than that in gastric pH (1.2). In gastric pH, the percentage of beads adhered onto the goat intestinal mucosal tissue varied from 64.88 ± 5.06% over 10 hours, whereas this was 30.47 ± 3.86% in intestinal pH (Figure 14). Thus, the results of the ex vivo wash-off test indicated that the newly developed optimized glibenclamide-loaded IHM-alginate beads had good mucoadhesivity.

Bottom Line: The effects of sodium alginate (SA) to IHM and cross-linker (CaCl2) concentration on the drug encapsulation efficiency (DEE, %), as well as cumulative drug release after 10 hours (R10 h, %), were optimized using 3(2) factorial design based on response surface methodology.The in vitro drug release from these beads was followed by controlled release (zero-order) pattern with super case-II transport mechanism.The optimized glibenclamide-loaded IHM-alginate mucoadhesive beads showed significant antidiabetic effect in alloxan-induced diabetic rats over prolonged period after oral administration.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Seemanta Institute of Pharmaceutical Sciences, Jharpokharia, Mayurbhanj, Odisha 757086, India.

ABSTRACT
The current study deals with the development and optimization of ispaghula (Plantago ovata F.) husk mucilage- (IHM-) alginate mucoadhesive beads containing glibenclamide by ionotropic gelation technique. The effects of sodium alginate (SA) to IHM and cross-linker (CaCl2) concentration on the drug encapsulation efficiency (DEE, %), as well as cumulative drug release after 10 hours (R10 h, %), were optimized using 3(2) factorial design based on response surface methodology. The observed responses were coincided well with the predicted values by the experimental design. The optimized mucoadhesive beads exhibited 94.43 ± 4.80% w/w of DEE and good mucoadhesivity with the biological membrane in wash-off test and sustained drug release profile over 10 hours. The beads were also characterized by SEM and FTIR analyses. The in vitro drug release from these beads was followed by controlled release (zero-order) pattern with super case-II transport mechanism. The optimized glibenclamide-loaded IHM-alginate mucoadhesive beads showed significant antidiabetic effect in alloxan-induced diabetic rats over prolonged period after oral administration.

No MeSH data available.