Limits...
HPLC Quantification of Phenolic Acids from Vetiveria zizanioides (L.) Nash and Its Antioxidant and Antimicrobial Activity.

Prajna J, Richa J, Dipjyoti C - J Pharm (Cairo) (2013)

Bottom Line: Antioxidant property expressed as percentage TEAC value obtained by ABTS assay was correlated with the amount of phenolic acids and showed a Pearson's coefficient 0.988 (significant at 0.01 level).The compounds p-coumaric acid, p-dihydroxybenzoic acid, and ferulic acid were detected in the acidic extracts by HPLC analysis.The plant extracts exhibited considerable antimicrobial activity against tested bacterial and fungal strains.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan 304022, India.

ABSTRACT
Extraction procedure was standardized and for the soluble, glycoside, and wall-bound fractions of phenolic acids from Vetiveria zizanioides. The water soluble alkaline extract which represents the cell wall-bound fraction contained the highest amount of phenolic acids (2.62 ± 1.2 μM/g fwt GA equivalents). Increased phenolic content in the cell wall indicates more lignin deposition which has an important role in plant defense and stress mitigation. Antioxidant property expressed as percentage TEAC value obtained by ABTS assay was correlated with the amount of phenolic acids and showed a Pearson's coefficient 0.988 (significant at 0.01 level). The compounds p-coumaric acid, p-dihydroxybenzoic acid, and ferulic acid were detected in the acidic extracts by HPLC analysis. The plant extracts exhibited considerable antimicrobial activity against tested bacterial and fungal strains.

No MeSH data available.


Related in: MedlinePlus

Antibacterial activity (LC50 values) of the phenolic extracts of V. zizanioides against the bacterial strains E. coli, B. subtilis, and M. luteus. HW: water soluble acidic fraction, HM: methanol soluble acidic fraction, NW: water soluble alkaline fraction, NM: methanol soluble alkaline fraction, and M: 80% methanolic extract.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4590811&req=5

fig2: Antibacterial activity (LC50 values) of the phenolic extracts of V. zizanioides against the bacterial strains E. coli, B. subtilis, and M. luteus. HW: water soluble acidic fraction, HM: methanol soluble acidic fraction, NW: water soluble alkaline fraction, NM: methanol soluble alkaline fraction, and M: 80% methanolic extract.

Mentions: The antibacterial activity of the plant extracts is represented in Figure 2. Initially the crude phenolic extracts were tested for their effectiveness against the bacterial strains in agar plate assay (results not shown). The quantification of antibacterial activity was done by broth assay method. In general, the extracts were more potent against the gram negative E. coli in comparison to B. subtilis and M. luteus, except for the alkaline methanol soluble fraction which was more potent against M. luteus and the methanol soluble acid extract which was more active against B. subtilis. The water soluble and methanol soluble alkaline extract showed the lowest LC50 values hence highest activity against E. coli. Alkaline extract dissolved in methanol showed highest activity against B. subtilis and M. luteus. The LC50 values of Kanamycin against E. coli, B. subtilis, and M. luteus were 146.36, 121.18, and 45.42 μg/mL, respectively.


HPLC Quantification of Phenolic Acids from Vetiveria zizanioides (L.) Nash and Its Antioxidant and Antimicrobial Activity.

Prajna J, Richa J, Dipjyoti C - J Pharm (Cairo) (2013)

Antibacterial activity (LC50 values) of the phenolic extracts of V. zizanioides against the bacterial strains E. coli, B. subtilis, and M. luteus. HW: water soluble acidic fraction, HM: methanol soluble acidic fraction, NW: water soluble alkaline fraction, NM: methanol soluble alkaline fraction, and M: 80% methanolic extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590811&req=5

fig2: Antibacterial activity (LC50 values) of the phenolic extracts of V. zizanioides against the bacterial strains E. coli, B. subtilis, and M. luteus. HW: water soluble acidic fraction, HM: methanol soluble acidic fraction, NW: water soluble alkaline fraction, NM: methanol soluble alkaline fraction, and M: 80% methanolic extract.
Mentions: The antibacterial activity of the plant extracts is represented in Figure 2. Initially the crude phenolic extracts were tested for their effectiveness against the bacterial strains in agar plate assay (results not shown). The quantification of antibacterial activity was done by broth assay method. In general, the extracts were more potent against the gram negative E. coli in comparison to B. subtilis and M. luteus, except for the alkaline methanol soluble fraction which was more potent against M. luteus and the methanol soluble acid extract which was more active against B. subtilis. The water soluble and methanol soluble alkaline extract showed the lowest LC50 values hence highest activity against E. coli. Alkaline extract dissolved in methanol showed highest activity against B. subtilis and M. luteus. The LC50 values of Kanamycin against E. coli, B. subtilis, and M. luteus were 146.36, 121.18, and 45.42 μg/mL, respectively.

Bottom Line: Antioxidant property expressed as percentage TEAC value obtained by ABTS assay was correlated with the amount of phenolic acids and showed a Pearson's coefficient 0.988 (significant at 0.01 level).The compounds p-coumaric acid, p-dihydroxybenzoic acid, and ferulic acid were detected in the acidic extracts by HPLC analysis.The plant extracts exhibited considerable antimicrobial activity against tested bacterial and fungal strains.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan 304022, India.

ABSTRACT
Extraction procedure was standardized and for the soluble, glycoside, and wall-bound fractions of phenolic acids from Vetiveria zizanioides. The water soluble alkaline extract which represents the cell wall-bound fraction contained the highest amount of phenolic acids (2.62 ± 1.2 μM/g fwt GA equivalents). Increased phenolic content in the cell wall indicates more lignin deposition which has an important role in plant defense and stress mitigation. Antioxidant property expressed as percentage TEAC value obtained by ABTS assay was correlated with the amount of phenolic acids and showed a Pearson's coefficient 0.988 (significant at 0.01 level). The compounds p-coumaric acid, p-dihydroxybenzoic acid, and ferulic acid were detected in the acidic extracts by HPLC analysis. The plant extracts exhibited considerable antimicrobial activity against tested bacterial and fungal strains.

No MeSH data available.


Related in: MedlinePlus