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Optimization of Metronidazole Emulgel.

Rao M, Sukre G, Aghav S, Kumar M - J Pharm (Cairo) (2013)

Bottom Line: The statistical validity of the polynomials was established, and optimized formulation factors were selected.Validation of the optimization study with 3 confirmatory runs indicated a high degree of prognostic ability of response surface methodology.Emulgel system of MTZ was developed and optimized using 2(3) factorial design and could provide an effective treatment against topical infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, AISSMS College of Pharmacy, Kennedy Road, Maharashtra, Pune 411 001, India.

ABSTRACT
The purpose of the present study was to develop and optimize the emulgel system for MTZ (Metronidazole), a poorly water soluble drug. The pseudoternary phase diagrams were developed for various microemulsion formulations composed of Capmul 908 P, Acconon MC8-2, and propylene glycol. The emulgel was optimized using a three-factor, two-level factorial design, the independent variables selected were Capmul 908 P, and surfactant mixture (Acconon MC8-2 and gelling agent), and the dependent variables (responses) were a cumulative amount of drug permeated across the dialysis membrane in 24 h (Y 1) and spreadability (Y 2). Mathematical equations and response surface plots were used to relate the dependent and independent variables. The regression equations were generated for responses Y 1 and Y 2. The statistical validity of the polynomials was established, and optimized formulation factors were selected. Validation of the optimization study with 3 confirmatory runs indicated a high degree of prognostic ability of response surface methodology. Emulgel system of MTZ was developed and optimized using 2(3) factorial design and could provide an effective treatment against topical infections.

No MeSH data available.


Related in: MedlinePlus

Pseudoternary phase diagrams of microemulsions composed of oil phase (Capmul 908 P), surfactant (Acconon), cosurfactant (propylene glycol), and water, where (a), (b), (c), and (d) were the different ratios of Smix 1 : 1, 2 : 1, 3 : 1, and 4 : 1, respectively.
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Related In: Results  -  Collection


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fig1: Pseudoternary phase diagrams of microemulsions composed of oil phase (Capmul 908 P), surfactant (Acconon), cosurfactant (propylene glycol), and water, where (a), (b), (c), and (d) were the different ratios of Smix 1 : 1, 2 : 1, 3 : 1, and 4 : 1, respectively.

Mentions: For the construction of pseudoternary phase diagrams, MEs, from the selected oil and surfactants, were prepared in different volume ratios (1 : 9, 1 : 8, 1 : 7, 1 : 6, 1 : 5, 1 : 4, 1 : 3, 1 : 2, and 1 : 1). Figure 1 presents the pseudoternary phase diagrams with various weight ratios of Acconon/propylene glycol (1 : 1, 2 : 1, 3 : 1, and 4 : 1). From Figure 1, it was found that the ME area was maximum at Smix ratio of 2 : 1. Hence this ratio was selected for preparation of drug-loaded MEs. At 1 : 1 ratio, the concentration of Acconon may not be sufficient to form a tightly packed barrier film. The ME region for 3 : 1 and 4 : 1 was significantly lesser than 1 : 1 and 2 : 1. Acconon is a C8 PEG-caprylic glyceride with HLB of 14 and molecular weight of 400 daltons. At higher concentration of Acconon, some of the molecules may be involved in formation of micelles. Micelles lie in the colloidal size range and hence may be contributing to the cloudiness of the dispersion. Thus we may presume that, for 3 : 1 and 4 : 1 ratios, reduced ME region is seen in the ternary plots.


Optimization of Metronidazole Emulgel.

Rao M, Sukre G, Aghav S, Kumar M - J Pharm (Cairo) (2013)

Pseudoternary phase diagrams of microemulsions composed of oil phase (Capmul 908 P), surfactant (Acconon), cosurfactant (propylene glycol), and water, where (a), (b), (c), and (d) were the different ratios of Smix 1 : 1, 2 : 1, 3 : 1, and 4 : 1, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590800&req=5

fig1: Pseudoternary phase diagrams of microemulsions composed of oil phase (Capmul 908 P), surfactant (Acconon), cosurfactant (propylene glycol), and water, where (a), (b), (c), and (d) were the different ratios of Smix 1 : 1, 2 : 1, 3 : 1, and 4 : 1, respectively.
Mentions: For the construction of pseudoternary phase diagrams, MEs, from the selected oil and surfactants, were prepared in different volume ratios (1 : 9, 1 : 8, 1 : 7, 1 : 6, 1 : 5, 1 : 4, 1 : 3, 1 : 2, and 1 : 1). Figure 1 presents the pseudoternary phase diagrams with various weight ratios of Acconon/propylene glycol (1 : 1, 2 : 1, 3 : 1, and 4 : 1). From Figure 1, it was found that the ME area was maximum at Smix ratio of 2 : 1. Hence this ratio was selected for preparation of drug-loaded MEs. At 1 : 1 ratio, the concentration of Acconon may not be sufficient to form a tightly packed barrier film. The ME region for 3 : 1 and 4 : 1 was significantly lesser than 1 : 1 and 2 : 1. Acconon is a C8 PEG-caprylic glyceride with HLB of 14 and molecular weight of 400 daltons. At higher concentration of Acconon, some of the molecules may be involved in formation of micelles. Micelles lie in the colloidal size range and hence may be contributing to the cloudiness of the dispersion. Thus we may presume that, for 3 : 1 and 4 : 1 ratios, reduced ME region is seen in the ternary plots.

Bottom Line: The statistical validity of the polynomials was established, and optimized formulation factors were selected.Validation of the optimization study with 3 confirmatory runs indicated a high degree of prognostic ability of response surface methodology.Emulgel system of MTZ was developed and optimized using 2(3) factorial design and could provide an effective treatment against topical infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, AISSMS College of Pharmacy, Kennedy Road, Maharashtra, Pune 411 001, India.

ABSTRACT
The purpose of the present study was to develop and optimize the emulgel system for MTZ (Metronidazole), a poorly water soluble drug. The pseudoternary phase diagrams were developed for various microemulsion formulations composed of Capmul 908 P, Acconon MC8-2, and propylene glycol. The emulgel was optimized using a three-factor, two-level factorial design, the independent variables selected were Capmul 908 P, and surfactant mixture (Acconon MC8-2 and gelling agent), and the dependent variables (responses) were a cumulative amount of drug permeated across the dialysis membrane in 24 h (Y 1) and spreadability (Y 2). Mathematical equations and response surface plots were used to relate the dependent and independent variables. The regression equations were generated for responses Y 1 and Y 2. The statistical validity of the polynomials was established, and optimized formulation factors were selected. Validation of the optimization study with 3 confirmatory runs indicated a high degree of prognostic ability of response surface methodology. Emulgel system of MTZ was developed and optimized using 2(3) factorial design and could provide an effective treatment against topical infections.

No MeSH data available.


Related in: MedlinePlus