Limits...
Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms by High-Performance Liquid Chromatography.

Ashour S, Kattan N - J Pharm (Cairo) (2013)

Bottom Line: Almotriptan (ALT) was used as internal standard.LOD and LOQ were 0.088 and 0.294 μg mL(-1) for CDB and 0.121 and 0.403 μg mL(-1) for CDZ, respectively.The proposed method was successfully applied to the determination of CDB and CDZ in combined dosage forms and the results tallied well with the label claim.

View Article: PubMed Central - PubMed

Affiliation: Analytical Biochemistry Laboratory, Department of Chemistry, Faculty of Science, University of Aleppo, Aleppo, Syria.

ABSTRACT
A sensitive and precise RP-HPLC method has been developed for the simultaneous estimation of clidinium bromide (CDB) and chlordiazepoxide (CDZ) in pure and pharmaceutical formulations. The separation was achieved on a Nucleodur C8 (250 × 4.6 mm i.d., 5 μm particle size) column at 25°C. CH3CN-MeOH-NH4OAc 0.1M (30 : 40 : 30, v/v/v) was used as the mobile phase at a flow rate of 1.0 mL min(-1) and detector wavelength at 218 nm. Almotriptan (ALT) was used as internal standard. The validation of the proposed method was carried out for linearity, accuracy, precision, LOD, LOQ, and robustness. The method showed good linearity in the ranges of 2.5-300.0 and 3.0-500.0 μg mL(-1) for CDB and CDZ, respectively. The percentage recovery obtained for CDB and CDZ was 100.40-103.38 and 99.98-105.59%, respectively. LOD and LOQ were 0.088 and 0.294 μg mL(-1) for CDB and 0.121 and 0.403 μg mL(-1) for CDZ, respectively. The proposed method was successfully applied to the determination of CDB and CDZ in combined dosage forms and the results tallied well with the label claim.

No MeSH data available.


Related in: MedlinePlus

Plots of the retention time versus methanol or acetonitrile percentage in the mobile phase of ALT, CDB, and CDZ.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4590797&req=5

fig1: Plots of the retention time versus methanol or acetonitrile percentage in the mobile phase of ALT, CDB, and CDZ.

Mentions: The optimized compositions were used for the analysis of all solutions individually as well as in combination. The mobile phase used initially was composed of ammonium acetate (0.1 M) and methanol. However, to achieve the optimum resolution, a small portion of acetonitrile was added in the mobile phase until obtaining good results. The chromatographic conditions were optimized for separation of drugs by varying methanol, strength of buffer solution, pH, proportion of acetonitrile, and flow rate. During the optimization of the method, different columns (Nucleodur C8, 250 × 4.6 mm, 5 μm; Nucleodur C18  250 × 4.6 mm, 5 μm; Hypersil Gold C8  250 × 4.6 mm, 5 μm; ODS Hypersil C18  250 × 4.6 mm, 5 μm) were tested. The chromatographic separation was achieved on Nucleodur C8, (250 × 4.6 mm, 5 μm) column at 25°C. The peak shape of ALT, CDB, and CDZ was found to be symmetrical. The effect of composition of the mobile phase and flow rate on the retention time of ALT, CDB, and CDZ was investigated. The effect of methanol and acetonitrile percentage in the mobile phase is presented in Figure 1.


Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms by High-Performance Liquid Chromatography.

Ashour S, Kattan N - J Pharm (Cairo) (2013)

Plots of the retention time versus methanol or acetonitrile percentage in the mobile phase of ALT, CDB, and CDZ.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590797&req=5

fig1: Plots of the retention time versus methanol or acetonitrile percentage in the mobile phase of ALT, CDB, and CDZ.
Mentions: The optimized compositions were used for the analysis of all solutions individually as well as in combination. The mobile phase used initially was composed of ammonium acetate (0.1 M) and methanol. However, to achieve the optimum resolution, a small portion of acetonitrile was added in the mobile phase until obtaining good results. The chromatographic conditions were optimized for separation of drugs by varying methanol, strength of buffer solution, pH, proportion of acetonitrile, and flow rate. During the optimization of the method, different columns (Nucleodur C8, 250 × 4.6 mm, 5 μm; Nucleodur C18  250 × 4.6 mm, 5 μm; Hypersil Gold C8  250 × 4.6 mm, 5 μm; ODS Hypersil C18  250 × 4.6 mm, 5 μm) were tested. The chromatographic separation was achieved on Nucleodur C8, (250 × 4.6 mm, 5 μm) column at 25°C. The peak shape of ALT, CDB, and CDZ was found to be symmetrical. The effect of composition of the mobile phase and flow rate on the retention time of ALT, CDB, and CDZ was investigated. The effect of methanol and acetonitrile percentage in the mobile phase is presented in Figure 1.

Bottom Line: Almotriptan (ALT) was used as internal standard.LOD and LOQ were 0.088 and 0.294 μg mL(-1) for CDB and 0.121 and 0.403 μg mL(-1) for CDZ, respectively.The proposed method was successfully applied to the determination of CDB and CDZ in combined dosage forms and the results tallied well with the label claim.

View Article: PubMed Central - PubMed

Affiliation: Analytical Biochemistry Laboratory, Department of Chemistry, Faculty of Science, University of Aleppo, Aleppo, Syria.

ABSTRACT
A sensitive and precise RP-HPLC method has been developed for the simultaneous estimation of clidinium bromide (CDB) and chlordiazepoxide (CDZ) in pure and pharmaceutical formulations. The separation was achieved on a Nucleodur C8 (250 × 4.6 mm i.d., 5 μm particle size) column at 25°C. CH3CN-MeOH-NH4OAc 0.1M (30 : 40 : 30, v/v/v) was used as the mobile phase at a flow rate of 1.0 mL min(-1) and detector wavelength at 218 nm. Almotriptan (ALT) was used as internal standard. The validation of the proposed method was carried out for linearity, accuracy, precision, LOD, LOQ, and robustness. The method showed good linearity in the ranges of 2.5-300.0 and 3.0-500.0 μg mL(-1) for CDB and CDZ, respectively. The percentage recovery obtained for CDB and CDZ was 100.40-103.38 and 99.98-105.59%, respectively. LOD and LOQ were 0.088 and 0.294 μg mL(-1) for CDB and 0.121 and 0.403 μg mL(-1) for CDZ, respectively. The proposed method was successfully applied to the determination of CDB and CDZ in combined dosage forms and the results tallied well with the label claim.

No MeSH data available.


Related in: MedlinePlus