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Correlation Between Nuclear Factor E2-Related Factor 2 Expression and Gastric Cancer Progression.

Zheng H, Nong Z, Lu G - Med. Sci. Monit. (2015)

Bottom Line: The correlation between Nfr2 expression and clinical indexes was further analyzed by Kaplan-Meier test, univariate/multivariate analysis, and log-rank test.Nrf2 is mainly expressed in nuclei of gastric carcinoma tissues, with significant correlation with clinical indexes, including tumor size, invasive depth, lymph node metastasis, and invasion.Patients with Nrf2-positive expression had significantly lower survival rates compared to those in the negative group (p<0.01), with chemo-resistance against 5-fluorouracil (5-FU) (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Ultrasound, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China (mainland).

ABSTRACT

Background: Nuclear factor E2-related factor 2 (Nrf2) plays an anti-oxidative and phase II detoxification function via its up-regulation on various antioxidant response elements (ARE) genes. Nrf2 can protect both normal and cancer cells from damages of cell stress, thereby exerting a critical role in the development of cancer. The expression and significance of Nrf2 in gastric cancer, however, has not been reported. This study thus aimed to investigate the expression of Nrf2 in gastric cancer tissues via immunohistochemical (IHC) staining.

Material and methods: Gastric carcinoma tissues from a total of 175 patients during surgical resection were examined for Nfr2 expression profiles using IHC staining on paraffin-embedded slides. Between-group-comparisons were performed by chi-square, Fisher's exact, or Mann-Whitney U test. The correlation between Nfr2 expression and clinical indexes was further analyzed by Kaplan-Meier test, univariate/multivariate analysis, and log-rank test.

Results: Nrf2 is mainly expressed in nuclei of gastric carcinoma tissues, with significant correlation with clinical indexes, including tumor size, invasive depth, lymph node metastasis, and invasion. Patients with Nrf2-positive expression had significantly lower survival rates compared to those in the negative group (p<0.01), with chemo-resistance against 5-fluorouracil (5-FU) (p<0.05).

Conclusions: Nrf2 expression is positively correlated with invasive gastric cancer, suggesting its utility as a predictive index for unfavorable prognosis.

No MeSH data available.


Related in: MedlinePlus

IHC staining of Nrf2 in stomach tissues. (A) Positive control staining in placenta; (B) Normal stomach tissues; (C) Negative staining for Nfr2; (D) Weak positive Nrf2; (E) Strong positive signals. Magnification: ×400.
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f2-medscimonit-21-2893: IHC staining of Nrf2 in stomach tissues. (A) Positive control staining in placenta; (B) Normal stomach tissues; (C) Negative staining for Nfr2; (D) Weak positive Nrf2; (E) Strong positive signals. Magnification: ×400.

Mentions: Table 1 lists general information and pathological indexes of all 175 patients. IHC staining obtained results consistent with those in the abovementioned Western blotting, as the Nrf2 signal localizes in the nucleus (Figure 2). Further examination revealed higher Nrf2 immune reactivity in undifferentiated cells. We calculated the staining scored as described in the methods section, and classified those with scores higher than 100 into the Nrf2-positive group. In all 175 patients, 108 were classified into the positive group (61.7%) and the other 67 were classified into the negative group (38.3%). Further correlation analysis between Nrf2 expression and clinical indexes are tabulated in Table 2. Significant correlations were discovered between Nrf2-positive expression and patient sex, tumor volume, infiltrative depth, lymph node metastasis, lymph tube invasion, and TNM staging.


Correlation Between Nuclear Factor E2-Related Factor 2 Expression and Gastric Cancer Progression.

Zheng H, Nong Z, Lu G - Med. Sci. Monit. (2015)

IHC staining of Nrf2 in stomach tissues. (A) Positive control staining in placenta; (B) Normal stomach tissues; (C) Negative staining for Nfr2; (D) Weak positive Nrf2; (E) Strong positive signals. Magnification: ×400.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4590579&req=5

f2-medscimonit-21-2893: IHC staining of Nrf2 in stomach tissues. (A) Positive control staining in placenta; (B) Normal stomach tissues; (C) Negative staining for Nfr2; (D) Weak positive Nrf2; (E) Strong positive signals. Magnification: ×400.
Mentions: Table 1 lists general information and pathological indexes of all 175 patients. IHC staining obtained results consistent with those in the abovementioned Western blotting, as the Nrf2 signal localizes in the nucleus (Figure 2). Further examination revealed higher Nrf2 immune reactivity in undifferentiated cells. We calculated the staining scored as described in the methods section, and classified those with scores higher than 100 into the Nrf2-positive group. In all 175 patients, 108 were classified into the positive group (61.7%) and the other 67 were classified into the negative group (38.3%). Further correlation analysis between Nrf2 expression and clinical indexes are tabulated in Table 2. Significant correlations were discovered between Nrf2-positive expression and patient sex, tumor volume, infiltrative depth, lymph node metastasis, lymph tube invasion, and TNM staging.

Bottom Line: The correlation between Nfr2 expression and clinical indexes was further analyzed by Kaplan-Meier test, univariate/multivariate analysis, and log-rank test.Nrf2 is mainly expressed in nuclei of gastric carcinoma tissues, with significant correlation with clinical indexes, including tumor size, invasive depth, lymph node metastasis, and invasion.Patients with Nrf2-positive expression had significantly lower survival rates compared to those in the negative group (p<0.01), with chemo-resistance against 5-fluorouracil (5-FU) (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Ultrasound, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China (mainland).

ABSTRACT

Background: Nuclear factor E2-related factor 2 (Nrf2) plays an anti-oxidative and phase II detoxification function via its up-regulation on various antioxidant response elements (ARE) genes. Nrf2 can protect both normal and cancer cells from damages of cell stress, thereby exerting a critical role in the development of cancer. The expression and significance of Nrf2 in gastric cancer, however, has not been reported. This study thus aimed to investigate the expression of Nrf2 in gastric cancer tissues via immunohistochemical (IHC) staining.

Material and methods: Gastric carcinoma tissues from a total of 175 patients during surgical resection were examined for Nfr2 expression profiles using IHC staining on paraffin-embedded slides. Between-group-comparisons were performed by chi-square, Fisher's exact, or Mann-Whitney U test. The correlation between Nfr2 expression and clinical indexes was further analyzed by Kaplan-Meier test, univariate/multivariate analysis, and log-rank test.

Results: Nrf2 is mainly expressed in nuclei of gastric carcinoma tissues, with significant correlation with clinical indexes, including tumor size, invasive depth, lymph node metastasis, and invasion. Patients with Nrf2-positive expression had significantly lower survival rates compared to those in the negative group (p<0.01), with chemo-resistance against 5-fluorouracil (5-FU) (p<0.05).

Conclusions: Nrf2 expression is positively correlated with invasive gastric cancer, suggesting its utility as a predictive index for unfavorable prognosis.

No MeSH data available.


Related in: MedlinePlus