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Long noncoding RNA CCAT2 promotes breast tumor growth by regulating the Wnt signaling pathway.

Cai Y, He J, Zhang D - Onco Targets Ther (2015)

Bottom Line: Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis.Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo.Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, Beijing City, People's Republic of China.

ABSTRACT
In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis. The lncRNA CCAT2 is dysregulated in several cancers such as colon cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, gastric cancer, and breast cancer; however, the contributions of CCAT2 to breast cancer remain largely unknown. In the current paper, we first confirmed the high expression level of CCAT2 in breast cancer tissues and breast cancer cell lines by reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, and we further analyzed the relationship between CCAT2 expression and clinical prognostic factors. Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo. Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway. In conclusion, lncRNA CCAT2 might be considered as a novel molecule involved in breast cancer development, which provides a potential therapeutic target for breast cancer.

No MeSH data available.


Related in: MedlinePlus

Suppressing CCAT2 expression inhibits tumorigenesis in vivo.Notes: The tumor xenograft model was performed to investigate the effects of si-CCAT2 on tumor formation. (A) Tumor size was calculated every 3 days after 7 days of injection. The data showed si-CCAT2 inhibited the tumor growth of MCF-7 cells in vivo. *P<0.05 vs the control. (B) Tumors were harvested at day 24, and the actual tumor size after harvest was shown. The data showed the xenograft tumors grew slower in the si-CCAT2 group. (C) Tumors were harvested and weighed at day 24. The data showed the tumor weight was less in the si-CCAT2 group. *P<0.05 vs the control (D) The relative expression level of CCAT2 was detected via RT-qPCR. The data showed the expression was decreased in the si-CCAT2 group. *P<0.05 versus the control.Abbreviations: si-CCAT2, siRNA specifically targeting CCAT2; d, day(s); si-NS, nonspecific siRNA.
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f4-ott-8-2657: Suppressing CCAT2 expression inhibits tumorigenesis in vivo.Notes: The tumor xenograft model was performed to investigate the effects of si-CCAT2 on tumor formation. (A) Tumor size was calculated every 3 days after 7 days of injection. The data showed si-CCAT2 inhibited the tumor growth of MCF-7 cells in vivo. *P<0.05 vs the control. (B) Tumors were harvested at day 24, and the actual tumor size after harvest was shown. The data showed the xenograft tumors grew slower in the si-CCAT2 group. (C) Tumors were harvested and weighed at day 24. The data showed the tumor weight was less in the si-CCAT2 group. *P<0.05 vs the control (D) The relative expression level of CCAT2 was detected via RT-qPCR. The data showed the expression was decreased in the si-CCAT2 group. *P<0.05 versus the control.Abbreviations: si-CCAT2, siRNA specifically targeting CCAT2; d, day(s); si-NS, nonspecific siRNA.

Mentions: To further determine the role of CCAT2 on tumorigenesis, MCF-7 cells transfected with either si-NS or si-CCAT2 mixed with matrigel were injected into nude mice. Two weeks after injection, a palpable tumor could be observed, and the results were consistent with the in vitro study; si-CCAT2 significantly reduced tumor growth at the indicated time (Figure 4A). In addition, tumors derived from the si-CCAT2 group grew at a slower rate than the si-NS group (Figure 4B), and the tumor weight in the si-CCAT2 group was significantly less than the si-NS group (Figure 4C). The expression level of CCAT2 was also detected, and the result showed that CCAT2 was knocked down effectively (Figure 4D).


Long noncoding RNA CCAT2 promotes breast tumor growth by regulating the Wnt signaling pathway.

Cai Y, He J, Zhang D - Onco Targets Ther (2015)

Suppressing CCAT2 expression inhibits tumorigenesis in vivo.Notes: The tumor xenograft model was performed to investigate the effects of si-CCAT2 on tumor formation. (A) Tumor size was calculated every 3 days after 7 days of injection. The data showed si-CCAT2 inhibited the tumor growth of MCF-7 cells in vivo. *P<0.05 vs the control. (B) Tumors were harvested at day 24, and the actual tumor size after harvest was shown. The data showed the xenograft tumors grew slower in the si-CCAT2 group. (C) Tumors were harvested and weighed at day 24. The data showed the tumor weight was less in the si-CCAT2 group. *P<0.05 vs the control (D) The relative expression level of CCAT2 was detected via RT-qPCR. The data showed the expression was decreased in the si-CCAT2 group. *P<0.05 versus the control.Abbreviations: si-CCAT2, siRNA specifically targeting CCAT2; d, day(s); si-NS, nonspecific siRNA.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4590572&req=5

f4-ott-8-2657: Suppressing CCAT2 expression inhibits tumorigenesis in vivo.Notes: The tumor xenograft model was performed to investigate the effects of si-CCAT2 on tumor formation. (A) Tumor size was calculated every 3 days after 7 days of injection. The data showed si-CCAT2 inhibited the tumor growth of MCF-7 cells in vivo. *P<0.05 vs the control. (B) Tumors were harvested at day 24, and the actual tumor size after harvest was shown. The data showed the xenograft tumors grew slower in the si-CCAT2 group. (C) Tumors were harvested and weighed at day 24. The data showed the tumor weight was less in the si-CCAT2 group. *P<0.05 vs the control (D) The relative expression level of CCAT2 was detected via RT-qPCR. The data showed the expression was decreased in the si-CCAT2 group. *P<0.05 versus the control.Abbreviations: si-CCAT2, siRNA specifically targeting CCAT2; d, day(s); si-NS, nonspecific siRNA.
Mentions: To further determine the role of CCAT2 on tumorigenesis, MCF-7 cells transfected with either si-NS or si-CCAT2 mixed with matrigel were injected into nude mice. Two weeks after injection, a palpable tumor could be observed, and the results were consistent with the in vitro study; si-CCAT2 significantly reduced tumor growth at the indicated time (Figure 4A). In addition, tumors derived from the si-CCAT2 group grew at a slower rate than the si-NS group (Figure 4B), and the tumor weight in the si-CCAT2 group was significantly less than the si-NS group (Figure 4C). The expression level of CCAT2 was also detected, and the result showed that CCAT2 was knocked down effectively (Figure 4D).

Bottom Line: Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis.Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo.Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, Beijing City, People's Republic of China.

ABSTRACT
In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis. The lncRNA CCAT2 is dysregulated in several cancers such as colon cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, gastric cancer, and breast cancer; however, the contributions of CCAT2 to breast cancer remain largely unknown. In the current paper, we first confirmed the high expression level of CCAT2 in breast cancer tissues and breast cancer cell lines by reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, and we further analyzed the relationship between CCAT2 expression and clinical prognostic factors. Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo. Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway. In conclusion, lncRNA CCAT2 might be considered as a novel molecule involved in breast cancer development, which provides a potential therapeutic target for breast cancer.

No MeSH data available.


Related in: MedlinePlus