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Long noncoding RNA CCAT2 promotes breast tumor growth by regulating the Wnt signaling pathway.

Cai Y, He J, Zhang D - Onco Targets Ther (2015)

Bottom Line: Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis.Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo.Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, Beijing City, People's Republic of China.

ABSTRACT
In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis. The lncRNA CCAT2 is dysregulated in several cancers such as colon cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, gastric cancer, and breast cancer; however, the contributions of CCAT2 to breast cancer remain largely unknown. In the current paper, we first confirmed the high expression level of CCAT2 in breast cancer tissues and breast cancer cell lines by reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, and we further analyzed the relationship between CCAT2 expression and clinical prognostic factors. Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo. Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway. In conclusion, lncRNA CCAT2 might be considered as a novel molecule involved in breast cancer development, which provides a potential therapeutic target for breast cancer.

No MeSH data available.


Related in: MedlinePlus

CCAT2 expression correlated with clinical prognostic factors.Notes: (A) According to the CCAT2 expression level in the tumor tissues, the involved breast cancer patients were divided into a low expression group and a high expression group, with an eightfold change (the average increase fold) as the demarcation point. (B) Breast cancer patients with high CCAT2 expression showed a significantly poorer prognosis than those with low CCAT2 expression according to the Kaplan–Meier overall survival curves. Con, tissue from normal person which was normalized to 1.
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f2-ott-8-2657: CCAT2 expression correlated with clinical prognostic factors.Notes: (A) According to the CCAT2 expression level in the tumor tissues, the involved breast cancer patients were divided into a low expression group and a high expression group, with an eightfold change (the average increase fold) as the demarcation point. (B) Breast cancer patients with high CCAT2 expression showed a significantly poorer prognosis than those with low CCAT2 expression according to the Kaplan–Meier overall survival curves. Con, tissue from normal person which was normalized to 1.

Mentions: To assess the correlation between CCAT2 expression and prognostic factors, we divided the breast cancer patients into two groups according to the CCAT2 expression level in the tumor tissues: low CCAT2 expression group (the change of relative expression < eightfold) and high CCAT2 expression group (the change of relative expression >eightfold) (Figure 2A). As shown in Figure 2B, patients with high CCAT2 expression had a significantly poorer prognosis than those with low expression, and the relative level of CCAT2 expression was correlated with overall survival rate of patients with breast cancer (Figure 2B). Overall, these observations indicated that increased CCAT2 expression is associated with the progression and development of breast cancer.


Long noncoding RNA CCAT2 promotes breast tumor growth by regulating the Wnt signaling pathway.

Cai Y, He J, Zhang D - Onco Targets Ther (2015)

CCAT2 expression correlated with clinical prognostic factors.Notes: (A) According to the CCAT2 expression level in the tumor tissues, the involved breast cancer patients were divided into a low expression group and a high expression group, with an eightfold change (the average increase fold) as the demarcation point. (B) Breast cancer patients with high CCAT2 expression showed a significantly poorer prognosis than those with low CCAT2 expression according to the Kaplan–Meier overall survival curves. Con, tissue from normal person which was normalized to 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590572&req=5

f2-ott-8-2657: CCAT2 expression correlated with clinical prognostic factors.Notes: (A) According to the CCAT2 expression level in the tumor tissues, the involved breast cancer patients were divided into a low expression group and a high expression group, with an eightfold change (the average increase fold) as the demarcation point. (B) Breast cancer patients with high CCAT2 expression showed a significantly poorer prognosis than those with low CCAT2 expression according to the Kaplan–Meier overall survival curves. Con, tissue from normal person which was normalized to 1.
Mentions: To assess the correlation between CCAT2 expression and prognostic factors, we divided the breast cancer patients into two groups according to the CCAT2 expression level in the tumor tissues: low CCAT2 expression group (the change of relative expression < eightfold) and high CCAT2 expression group (the change of relative expression >eightfold) (Figure 2A). As shown in Figure 2B, patients with high CCAT2 expression had a significantly poorer prognosis than those with low expression, and the relative level of CCAT2 expression was correlated with overall survival rate of patients with breast cancer (Figure 2B). Overall, these observations indicated that increased CCAT2 expression is associated with the progression and development of breast cancer.

Bottom Line: Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis.Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo.Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, Beijing City, People's Republic of China.

ABSTRACT
In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis. The lncRNA CCAT2 is dysregulated in several cancers such as colon cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, gastric cancer, and breast cancer; however, the contributions of CCAT2 to breast cancer remain largely unknown. In the current paper, we first confirmed the high expression level of CCAT2 in breast cancer tissues and breast cancer cell lines by reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, and we further analyzed the relationship between CCAT2 expression and clinical prognostic factors. Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo. Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway. In conclusion, lncRNA CCAT2 might be considered as a novel molecule involved in breast cancer development, which provides a potential therapeutic target for breast cancer.

No MeSH data available.


Related in: MedlinePlus