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Long noncoding RNA CCAT2 promotes breast tumor growth by regulating the Wnt signaling pathway.

Cai Y, He J, Zhang D - Onco Targets Ther (2015)

Bottom Line: In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs).Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo.Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, Beijing City, People's Republic of China.

ABSTRACT
In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis. The lncRNA CCAT2 is dysregulated in several cancers such as colon cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, gastric cancer, and breast cancer; however, the contributions of CCAT2 to breast cancer remain largely unknown. In the current paper, we first confirmed the high expression level of CCAT2 in breast cancer tissues and breast cancer cell lines by reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, and we further analyzed the relationship between CCAT2 expression and clinical prognostic factors. Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo. Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway. In conclusion, lncRNA CCAT2 might be considered as a novel molecule involved in breast cancer development, which provides a potential therapeutic target for breast cancer.

No MeSH data available.


Related in: MedlinePlus

CCAT2 is upregulated in breast tumors.Notes: (A) The CCAT2 expression levels in breast cancer samples were significantly higher than those in adjacent non-tumor tissues by RT-qPCR assay. GAPDH was used as an internal control. (B) Higher expression levels of CCAT2 were detected in MCF-7 and MDA-MB-231 cells than in normal Hs578Bst cells by RT-qPCR assay. GAPDH was used as an internal control. The experiments were all repeated at least three times. *P<0.05 vs the control, #P<0.01.Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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f1-ott-8-2657: CCAT2 is upregulated in breast tumors.Notes: (A) The CCAT2 expression levels in breast cancer samples were significantly higher than those in adjacent non-tumor tissues by RT-qPCR assay. GAPDH was used as an internal control. (B) Higher expression levels of CCAT2 were detected in MCF-7 and MDA-MB-231 cells than in normal Hs578Bst cells by RT-qPCR assay. GAPDH was used as an internal control. The experiments were all repeated at least three times. *P<0.05 vs the control, #P<0.01.Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Mentions: First, we measured the CCAT2 expression levels by RT-qPCR in a set of 67 matched samples. The result showed that CCAT2 appeared to have higher expression in breast cancer tissues than in adjacent non-tumor tissues (P<0.05) (Figure 1A). The CCAT2 expression was also examined by RT-qPCR in human breast cancer cell lines MDA-MB-231 and MCF-7. The normal mammary fibroblast cell line Hs578Bst was used as the control. This experiment showed that CCAT2 expression was higher in breast cancer cell lines than in normal mammary fibroblasts (P<0.001) (Figure 1B).


Long noncoding RNA CCAT2 promotes breast tumor growth by regulating the Wnt signaling pathway.

Cai Y, He J, Zhang D - Onco Targets Ther (2015)

CCAT2 is upregulated in breast tumors.Notes: (A) The CCAT2 expression levels in breast cancer samples were significantly higher than those in adjacent non-tumor tissues by RT-qPCR assay. GAPDH was used as an internal control. (B) Higher expression levels of CCAT2 were detected in MCF-7 and MDA-MB-231 cells than in normal Hs578Bst cells by RT-qPCR assay. GAPDH was used as an internal control. The experiments were all repeated at least three times. *P<0.05 vs the control, #P<0.01.Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590572&req=5

f1-ott-8-2657: CCAT2 is upregulated in breast tumors.Notes: (A) The CCAT2 expression levels in breast cancer samples were significantly higher than those in adjacent non-tumor tissues by RT-qPCR assay. GAPDH was used as an internal control. (B) Higher expression levels of CCAT2 were detected in MCF-7 and MDA-MB-231 cells than in normal Hs578Bst cells by RT-qPCR assay. GAPDH was used as an internal control. The experiments were all repeated at least three times. *P<0.05 vs the control, #P<0.01.Abbreviations: RT-qPCR, reverse transcription quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Mentions: First, we measured the CCAT2 expression levels by RT-qPCR in a set of 67 matched samples. The result showed that CCAT2 appeared to have higher expression in breast cancer tissues than in adjacent non-tumor tissues (P<0.05) (Figure 1A). The CCAT2 expression was also examined by RT-qPCR in human breast cancer cell lines MDA-MB-231 and MCF-7. The normal mammary fibroblast cell line Hs578Bst was used as the control. This experiment showed that CCAT2 expression was higher in breast cancer cell lines than in normal mammary fibroblasts (P<0.001) (Figure 1B).

Bottom Line: In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs).Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo.Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, Beijing City, People's Republic of China.

ABSTRACT
In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs, including microRNAs and long noncoding RNAs (lncRNAs). Emerging evidence indicates that lncRNAs could have a critical role in the regulation of cellular processes such as cell growth and apoptosis as well as cancer progression and metastasis. The lncRNA CCAT2 is dysregulated in several cancers such as colon cancer, non-small cell lung cancer, esophageal squamous cell carcinoma, gastric cancer, and breast cancer; however, the contributions of CCAT2 to breast cancer remain largely unknown. In the current paper, we first confirmed the high expression level of CCAT2 in breast cancer tissues and breast cancer cell lines by reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, and we further analyzed the relationship between CCAT2 expression and clinical prognostic factors. Also, the biological function of CCAT2 was explored and the results showed silencing of CCAT2 could suppress cell growth in vitro and tumor formation in vivo. Finally, our results revealed that the abnormal expression of CCAT2 could influence the Wnt signaling pathway. In conclusion, lncRNA CCAT2 might be considered as a novel molecule involved in breast cancer development, which provides a potential therapeutic target for breast cancer.

No MeSH data available.


Related in: MedlinePlus