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Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus

Antibacterial potential of AgNPs by colony counting method.Notes:Escherichia coli without treatment (just water) (A), E. coli treated with plant extract (B), E. coli treated with AgNO3 (C), and E. coli treated with AgNPs (D).Abbreviation: AgNPs, silver nanoparticles.
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f7-ijn-10-5955: Antibacterial potential of AgNPs by colony counting method.Notes:Escherichia coli without treatment (just water) (A), E. coli treated with plant extract (B), E. coli treated with AgNO3 (C), and E. coli treated with AgNPs (D).Abbreviation: AgNPs, silver nanoparticles.

Mentions: It is well known that a number of chemical forms of silver exhibit antimicrobial activities.25 AgNPs showed a wider bacterial inhibition zone than AgNO3 in all the analyzed bacteria (E. coli, P. aeruginosa, and A. tumefaciens) (Figure 5A–C). The interaction of AgNPs with E. coli cells were studied using SEM analysis (Figure 6). The AgNPs treated cells (2 hours and 4 hours) showed increased disruption in the cell wall of E. coli cells with increased time interval. No disruption was seen in untreated cells, clearly indicating the enhanced antibacterial activity of AgNPs (Figure 6A–C). The results from colony counting method showed that the AgNPs significantly reduced the number of E. coli cells from 106 to 1.3±0.9 CFUs/mL, while the reduction with AgNO3 treatment was to 258.6±9.9 CFUs/mL, and no reduction was observed with WS aqueous extract treatment (Figure 7). These results further substantiate the higher antimicrobial activity (~200 times more effective) of AgNPs compared with equivalent amounts of AgNO3. It has been reported that AgNPs show higher activity then their AgNO3 counterparts.26 Moreover, AgNPs biosynthesized from plant extracts showed enhanced antibacterial activity compared with chemically synthesized AgNPs.27


Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Antibacterial potential of AgNPs by colony counting method.Notes:Escherichia coli without treatment (just water) (A), E. coli treated with plant extract (B), E. coli treated with AgNO3 (C), and E. coli treated with AgNPs (D).Abbreviation: AgNPs, silver nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590548&req=5

f7-ijn-10-5955: Antibacterial potential of AgNPs by colony counting method.Notes:Escherichia coli without treatment (just water) (A), E. coli treated with plant extract (B), E. coli treated with AgNO3 (C), and E. coli treated with AgNPs (D).Abbreviation: AgNPs, silver nanoparticles.
Mentions: It is well known that a number of chemical forms of silver exhibit antimicrobial activities.25 AgNPs showed a wider bacterial inhibition zone than AgNO3 in all the analyzed bacteria (E. coli, P. aeruginosa, and A. tumefaciens) (Figure 5A–C). The interaction of AgNPs with E. coli cells were studied using SEM analysis (Figure 6). The AgNPs treated cells (2 hours and 4 hours) showed increased disruption in the cell wall of E. coli cells with increased time interval. No disruption was seen in untreated cells, clearly indicating the enhanced antibacterial activity of AgNPs (Figure 6A–C). The results from colony counting method showed that the AgNPs significantly reduced the number of E. coli cells from 106 to 1.3±0.9 CFUs/mL, while the reduction with AgNO3 treatment was to 258.6±9.9 CFUs/mL, and no reduction was observed with WS aqueous extract treatment (Figure 7). These results further substantiate the higher antimicrobial activity (~200 times more effective) of AgNPs compared with equivalent amounts of AgNO3. It has been reported that AgNPs show higher activity then their AgNO3 counterparts.26 Moreover, AgNPs biosynthesized from plant extracts showed enhanced antibacterial activity compared with chemically synthesized AgNPs.27

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus