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Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatograms of aqueous leaf extract of Withania somnifera and green synthesized AgNPs.Notes: (A and B) HPLC chromatogram of aqueous leaf extract of Withania somnifera recorded at 235 and 350 nm. W1–W6 are whitanolide-type compounds. (C and D) HPLC chromatogram of AgNPs recorded at 235 and 350 nm. Peak 1 indicates catechin; peak 2 indicates p-coumaric acid; peak 3 indicates luteolin-7-glucoside; and peak 4 indicates a whitanolide-type compound.Abbreviations: AgNPs, silver nanoparticles; HPLC, high-performance liquid chromatography.
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f4-ijn-10-5955: HPLC chromatograms of aqueous leaf extract of Withania somnifera and green synthesized AgNPs.Notes: (A and B) HPLC chromatogram of aqueous leaf extract of Withania somnifera recorded at 235 and 350 nm. W1–W6 are whitanolide-type compounds. (C and D) HPLC chromatogram of AgNPs recorded at 235 and 350 nm. Peak 1 indicates catechin; peak 2 indicates p-coumaric acid; peak 3 indicates luteolin-7-glucoside; and peak 4 indicates a whitanolide-type compound.Abbreviations: AgNPs, silver nanoparticles; HPLC, high-performance liquid chromatography.

Mentions: In order to identify the WS compounds participating in green synthesis of AgNPs, we performed HPLC analysis. We identified several withanolides in WS leaf extract based on their characteristic UV-vis spectra (Figure 4A–D; Table 1). In addition, HPLC analysis of AgNPs revealed that some of the phenolic compounds present in the WS leaf extract were selectively trapped in the nanoparticles (Figure 4C and D). These compounds, based on their characteristic UV-vis spectra and further confirmation by coeluting with pure standards, were identified as catechin, p-coumaric acid, and luteolin-7-glucoside (Figure 4C and D and Table 1). These substances were masked by other major compounds (peaks) present in the WS extract, namely withanolides; the compounds catechin, p-coumaric acid, and luteolin-7-glucoside were noticed in chromatograms of AgNPs as they were selectively trapped in AgNPs, and the other compounds were removed when the nanoparticles were washed. In addition, a major compound (peak 4) was found in AgNPs that was not identified in the original WS leaf aqueous extract. This compound had UV-vis spectra similar to other withanolides found in the leaf aqueous extract (peaks W2, W5, and W6) but with a different retention time and showing a bathochromic effect on its spectra. Taking this into consideration, this compound might be a derivative that originated from the interaction of some withanolide derivative with silver ions. So, we can conclude that the formation of AgNPs involved the interaction of silver ions with both selected phenolics and withanolides present in the WS aqueous leaf extract. Recently, the relevance of phenolic compounds for the green synthesis of metal nanoparticles from Eucalyptus globulus bark was reported.24


Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

HPLC chromatograms of aqueous leaf extract of Withania somnifera and green synthesized AgNPs.Notes: (A and B) HPLC chromatogram of aqueous leaf extract of Withania somnifera recorded at 235 and 350 nm. W1–W6 are whitanolide-type compounds. (C and D) HPLC chromatogram of AgNPs recorded at 235 and 350 nm. Peak 1 indicates catechin; peak 2 indicates p-coumaric acid; peak 3 indicates luteolin-7-glucoside; and peak 4 indicates a whitanolide-type compound.Abbreviations: AgNPs, silver nanoparticles; HPLC, high-performance liquid chromatography.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590548&req=5

f4-ijn-10-5955: HPLC chromatograms of aqueous leaf extract of Withania somnifera and green synthesized AgNPs.Notes: (A and B) HPLC chromatogram of aqueous leaf extract of Withania somnifera recorded at 235 and 350 nm. W1–W6 are whitanolide-type compounds. (C and D) HPLC chromatogram of AgNPs recorded at 235 and 350 nm. Peak 1 indicates catechin; peak 2 indicates p-coumaric acid; peak 3 indicates luteolin-7-glucoside; and peak 4 indicates a whitanolide-type compound.Abbreviations: AgNPs, silver nanoparticles; HPLC, high-performance liquid chromatography.
Mentions: In order to identify the WS compounds participating in green synthesis of AgNPs, we performed HPLC analysis. We identified several withanolides in WS leaf extract based on their characteristic UV-vis spectra (Figure 4A–D; Table 1). In addition, HPLC analysis of AgNPs revealed that some of the phenolic compounds present in the WS leaf extract were selectively trapped in the nanoparticles (Figure 4C and D). These compounds, based on their characteristic UV-vis spectra and further confirmation by coeluting with pure standards, were identified as catechin, p-coumaric acid, and luteolin-7-glucoside (Figure 4C and D and Table 1). These substances were masked by other major compounds (peaks) present in the WS extract, namely withanolides; the compounds catechin, p-coumaric acid, and luteolin-7-glucoside were noticed in chromatograms of AgNPs as they were selectively trapped in AgNPs, and the other compounds were removed when the nanoparticles were washed. In addition, a major compound (peak 4) was found in AgNPs that was not identified in the original WS leaf aqueous extract. This compound had UV-vis spectra similar to other withanolides found in the leaf aqueous extract (peaks W2, W5, and W6) but with a different retention time and showing a bathochromic effect on its spectra. Taking this into consideration, this compound might be a derivative that originated from the interaction of some withanolide derivative with silver ions. So, we can conclude that the formation of AgNPs involved the interaction of silver ions with both selected phenolics and withanolides present in the WS aqueous leaf extract. Recently, the relevance of phenolic compounds for the green synthesis of metal nanoparticles from Eucalyptus globulus bark was reported.24

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus