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Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus

XRD patterns of the synthesized AgNPs with aqueous leaf extract of Withania Somnifera.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.
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f3-ijn-10-5955: XRD patterns of the synthesized AgNPs with aqueous leaf extract of Withania Somnifera.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.

Mentions: The crystalline nature of AgNPs was confirmed by X-ray diffraction analysis (Figure 3). The intensity of peaks reflects the high degree of crystallinity of the AgNPs. The distinct diffraction peaks at 38.06, 44.23, and 67.43 were indexed with planes (111), (200), and (220). The intense X-ray diffraction pattern clearly showed the formation of AgNPs (JCPDS card no 04-0783, 1991).20 Amutha et al reported the same pattern of diffraction peak at 32.16 and indexed with plane (101) in bio-synthesized AgNPs at room temperature. In the same way, we got a peak at 32.20 which was indexed with plane (101).21


Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

XRD patterns of the synthesized AgNPs with aqueous leaf extract of Withania Somnifera.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590548&req=5

f3-ijn-10-5955: XRD patterns of the synthesized AgNPs with aqueous leaf extract of Withania Somnifera.Abbreviations: XRD, X-ray diffraction; AgNPs, silver nanoparticles.
Mentions: The crystalline nature of AgNPs was confirmed by X-ray diffraction analysis (Figure 3). The intensity of peaks reflects the high degree of crystallinity of the AgNPs. The distinct diffraction peaks at 38.06, 44.23, and 67.43 were indexed with planes (111), (200), and (220). The intense X-ray diffraction pattern clearly showed the formation of AgNPs (JCPDS card no 04-0783, 1991).20 Amutha et al reported the same pattern of diffraction peak at 32.16 and indexed with plane (101) in bio-synthesized AgNPs at room temperature. In the same way, we got a peak at 32.20 which was indexed with plane (101).21

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus