Limits...
Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus

Morphological characterization of AgNPs.Notes: Particle size distribution (A), TEM image (B), AFM height image (C), SEM primary image (D), backscattered image (E), and X-ray energy dispersive spectrum (F), of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles; AFM, atomic force microscopy; SEM, scanning electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4590548&req=5

f2-ijn-10-5955: Morphological characterization of AgNPs.Notes: Particle size distribution (A), TEM image (B), AFM height image (C), SEM primary image (D), backscattered image (E), and X-ray energy dispersive spectrum (F), of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles; AFM, atomic force microscopy; SEM, scanning electron microscopy.

Mentions: Particle size and size distribution are important characteristics of nanoparticles, as they determine their biological fate, toxicity, targeting ability, and stability. The synthesized AgNPs had a size ranging between 70 and 110 nm, as confirmed by laser Doppler anemometry analysis (Figure 2A). The zeta potential was found to be −30.0±1.8 mV, indicating the stability of the formulation.


Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Morphological characterization of AgNPs.Notes: Particle size distribution (A), TEM image (B), AFM height image (C), SEM primary image (D), backscattered image (E), and X-ray energy dispersive spectrum (F), of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles; AFM, atomic force microscopy; SEM, scanning electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590548&req=5

f2-ijn-10-5955: Morphological characterization of AgNPs.Notes: Particle size distribution (A), TEM image (B), AFM height image (C), SEM primary image (D), backscattered image (E), and X-ray energy dispersive spectrum (F), of AgNPs.Abbreviations: TEM, transmission electron microscopy; AgNPs, silver nanoparticles; AFM, atomic force microscopy; SEM, scanning electron microscopy.
Mentions: Particle size and size distribution are important characteristics of nanoparticles, as they determine their biological fate, toxicity, targeting ability, and stability. The synthesized AgNPs had a size ranging between 70 and 110 nm, as confirmed by laser Doppler anemometry analysis (Figure 2A). The zeta potential was found to be −30.0±1.8 mV, indicating the stability of the formulation.

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus