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Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus

Biosynthesis of AgNPs.Notes: Photograph showing the formation of AgNPs using Withania somnifera extract and visual observation of color changes (A). UV-vis spectra of AgNPs synthesized using 6% w/w Withania somnifera aqueous extract with 1 mM AgNO3 at different time intervals (B).Abbreviations: AgNPs, silver nanoparticles; OD, optical density; UV-vis, ultraviolet-visible.
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f1-ijn-10-5955: Biosynthesis of AgNPs.Notes: Photograph showing the formation of AgNPs using Withania somnifera extract and visual observation of color changes (A). UV-vis spectra of AgNPs synthesized using 6% w/w Withania somnifera aqueous extract with 1 mM AgNO3 at different time intervals (B).Abbreviations: AgNPs, silver nanoparticles; OD, optical density; UV-vis, ultraviolet-visible.

Mentions: Among the different concentrations of WS extracts and AgNO3 used for the synthesis of nanoparticles, 6% w/v WS and 1 mM AgNO3 showed better reaction profiles and were selected for further studies. When AgNO3 was added, leaf aqueous extract changed into reddish color (Figure 1A), whereas fruit and root extracts did not change their color within 7 days. UV-vis absorbance at 430 nm confirmed the reduction of silver ion to silver metal due to the reaction with the components present in the aqueous leaf extract of WS (Figure 1B). It was evident that the WS leaf extract contains compounds suitable for green synthesis, whereas root and fruit extracts do not. Recently, the photosensitized synthesis of AgNPs via using WS leaf powder was reported.19


Antimicrobial activity of cream incorporated with silver nanoparticles biosynthesized from Withania somnifera.

Marslin G, Selvakesavan RK, Franklin G, Sarmento B, Dias AC - Int J Nanomedicine (2015)

Biosynthesis of AgNPs.Notes: Photograph showing the formation of AgNPs using Withania somnifera extract and visual observation of color changes (A). UV-vis spectra of AgNPs synthesized using 6% w/w Withania somnifera aqueous extract with 1 mM AgNO3 at different time intervals (B).Abbreviations: AgNPs, silver nanoparticles; OD, optical density; UV-vis, ultraviolet-visible.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590548&req=5

f1-ijn-10-5955: Biosynthesis of AgNPs.Notes: Photograph showing the formation of AgNPs using Withania somnifera extract and visual observation of color changes (A). UV-vis spectra of AgNPs synthesized using 6% w/w Withania somnifera aqueous extract with 1 mM AgNO3 at different time intervals (B).Abbreviations: AgNPs, silver nanoparticles; OD, optical density; UV-vis, ultraviolet-visible.
Mentions: Among the different concentrations of WS extracts and AgNO3 used for the synthesis of nanoparticles, 6% w/v WS and 1 mM AgNO3 showed better reaction profiles and were selected for further studies. When AgNO3 was added, leaf aqueous extract changed into reddish color (Figure 1A), whereas fruit and root extracts did not change their color within 7 days. UV-vis absorbance at 430 nm confirmed the reduction of silver ion to silver metal due to the reaction with the components present in the aqueous leaf extract of WS (Figure 1B). It was evident that the WS leaf extract contains compounds suitable for green synthesis, whereas root and fruit extracts do not. Recently, the photosensitized synthesis of AgNPs via using WS leaf powder was reported.19

Bottom Line: Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera.After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream.Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

View Article: PubMed Central - PubMed

Affiliation: Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB-UM), AgroBioPlant Group, Department of Biology, University of Minho, Braga, Portugal.

ABSTRACT
We report on the antimicrobial activity of a cream formulation of silver nanoparticles (AgNPs), biosynthesized using Withania somnifera extract. Aqueous extracts of leaves promoted efficient green synthesis of AgNPs compared to fruits and root extracts of W. somnifera. Biosynthesized AgNPs were characterized for their size and shape by physical-chemical techniques such as UV-visible spectroscopy, laser Doppler anemometry, transmission electron microscopy, scanning electron microscopy, atomic force microscopy, X-ray diffraction, and X-ray energy dispersive spectroscopy. After confirming the antimicrobial potential of AgNPs, they were incorporated into a cream. Cream formulations of AgNPs and AgNO3 were prepared and compared for their antimicrobial activity against human pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, and Candida albicans) and a plant pathogen (Agrobacterium tumefaciens). Our results show that AgNP creams possess significantly higher antimicrobial activity against the tested organisms.

No MeSH data available.


Related in: MedlinePlus