Limits...
Bioinspired membrane-based systems for a physical approach of cell organization and dynamics: usefulness and limitations.

Lagny TJ, Bassereau P - Interface Focus (2015)

Bottom Line: Model membrane systems are also used in synthetic biology and can have potential applications beyond basic research.We discuss the possible synergy between the development of complex in vitro membrane systems in a biological context and for technological applications.Questions that could also be discussed are: what can we still do with synthetic systems, where do we stop building up and which are the alternative solutions?

View Article: PubMed Central - PubMed

Affiliation: Institut Curie, PSL Research University , Laboratory PhysicoChimie Curie , 75248 Paris, Cedex 05 , France ; CNRS , UMR168, 75248 Paris, Cedex 05 , France ; Université Pierre et Marie Curie , 75252 Paris, Cedex 05 , France.

ABSTRACT
Being at the periphery of each cell compartment and enclosing the entire cell while interacting with a large part of cell components, cell membranes participate in most of the cell's vital functions. Biologists have worked for a long time on deciphering how membranes are organized, how they contribute to trafficking, motility, cytokinesis, cell-cell communication, information transport, etc., using top-down approaches and always more advanced techniques. In contrast, physicists have developed bottom-up approaches and minimal model membrane systems of growing complexity in order to build up general models that explain how cell membranes work and how they interact with proteins, e.g. the cytoskeleton. We review the different model membrane systems that are currently available, and how they can help deciphering cell functioning, but also list their limitations. Model membrane systems are also used in synthetic biology and can have potential applications beyond basic research. We discuss the possible synergy between the development of complex in vitro membrane systems in a biological context and for technological applications. Questions that could also be discussed are: what can we still do with synthetic systems, where do we stop building up and which are the alternative solutions?

No MeSH data available.


Application of model-membrane systems. (a) Functionalized liposomes filled with pharmaceutical compounds can be used for targeted drug delivery. (b) Lipid bilayer systems with reconstituted transmembrane proteins, e.g. ion channels, offer high throughput in the screening of compounds that are modulating the gating of these channels. (c) Confining in vitro protein synthesis in the small volume of GUVs allows for straightforward production of highly concentrated products without the need for prolonged purification.
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RSFS20150038F2: Application of model-membrane systems. (a) Functionalized liposomes filled with pharmaceutical compounds can be used for targeted drug delivery. (b) Lipid bilayer systems with reconstituted transmembrane proteins, e.g. ion channels, offer high throughput in the screening of compounds that are modulating the gating of these channels. (c) Confining in vitro protein synthesis in the small volume of GUVs allows for straightforward production of highly concentrated products without the need for prolonged purification.

Mentions: Liposomes are commonly used as drug transporters with encapsulated pharmaceutical products (figure 2a). Considerable efforts have been made on the grafting of hydrophilic polymers (polyethylene glycol) on their membrane to extend their circulation lifetime in the body and prevent their rapid elimination by the immune system. Many ‘stealth’ liposome formulations are on the market [76]. Their efficiency can be increased by adding specific ligands for targeting the liposome delivery to a specific organ that has to be treated [77]. Lipids can be replaced by copolymers and liposomes can be controlled to release their content only upon specific stimuli, e.g. external ones such as light or internal ones such as pH [78].Figure 2.


Bioinspired membrane-based systems for a physical approach of cell organization and dynamics: usefulness and limitations.

Lagny TJ, Bassereau P - Interface Focus (2015)

Application of model-membrane systems. (a) Functionalized liposomes filled with pharmaceutical compounds can be used for targeted drug delivery. (b) Lipid bilayer systems with reconstituted transmembrane proteins, e.g. ion channels, offer high throughput in the screening of compounds that are modulating the gating of these channels. (c) Confining in vitro protein synthesis in the small volume of GUVs allows for straightforward production of highly concentrated products without the need for prolonged purification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590427&req=5

RSFS20150038F2: Application of model-membrane systems. (a) Functionalized liposomes filled with pharmaceutical compounds can be used for targeted drug delivery. (b) Lipid bilayer systems with reconstituted transmembrane proteins, e.g. ion channels, offer high throughput in the screening of compounds that are modulating the gating of these channels. (c) Confining in vitro protein synthesis in the small volume of GUVs allows for straightforward production of highly concentrated products without the need for prolonged purification.
Mentions: Liposomes are commonly used as drug transporters with encapsulated pharmaceutical products (figure 2a). Considerable efforts have been made on the grafting of hydrophilic polymers (polyethylene glycol) on their membrane to extend their circulation lifetime in the body and prevent their rapid elimination by the immune system. Many ‘stealth’ liposome formulations are on the market [76]. Their efficiency can be increased by adding specific ligands for targeting the liposome delivery to a specific organ that has to be treated [77]. Lipids can be replaced by copolymers and liposomes can be controlled to release their content only upon specific stimuli, e.g. external ones such as light or internal ones such as pH [78].Figure 2.

Bottom Line: Model membrane systems are also used in synthetic biology and can have potential applications beyond basic research.We discuss the possible synergy between the development of complex in vitro membrane systems in a biological context and for technological applications.Questions that could also be discussed are: what can we still do with synthetic systems, where do we stop building up and which are the alternative solutions?

View Article: PubMed Central - PubMed

Affiliation: Institut Curie, PSL Research University , Laboratory PhysicoChimie Curie , 75248 Paris, Cedex 05 , France ; CNRS , UMR168, 75248 Paris, Cedex 05 , France ; Université Pierre et Marie Curie , 75252 Paris, Cedex 05 , France.

ABSTRACT
Being at the periphery of each cell compartment and enclosing the entire cell while interacting with a large part of cell components, cell membranes participate in most of the cell's vital functions. Biologists have worked for a long time on deciphering how membranes are organized, how they contribute to trafficking, motility, cytokinesis, cell-cell communication, information transport, etc., using top-down approaches and always more advanced techniques. In contrast, physicists have developed bottom-up approaches and minimal model membrane systems of growing complexity in order to build up general models that explain how cell membranes work and how they interact with proteins, e.g. the cytoskeleton. We review the different model membrane systems that are currently available, and how they can help deciphering cell functioning, but also list their limitations. Model membrane systems are also used in synthetic biology and can have potential applications beyond basic research. We discuss the possible synergy between the development of complex in vitro membrane systems in a biological context and for technological applications. Questions that could also be discussed are: what can we still do with synthetic systems, where do we stop building up and which are the alternative solutions?

No MeSH data available.