Limits...
Effect of curcumin on aging retinal pigment epithelial cells.

Zhu W, Wu Y, Meng YF, Wang JY, Xu M, Tao JJ, Lu J - Drug Des Devel Ther (2015)

Bottom Line: Curcumin improved cell viability and decreased apoptosis and oxidative stress.Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers.In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Changshu No 2 People's Hospital, Changshu, People's Republic of China.

ABSTRACT
Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population. The antioxidative effects of curcumin on aging retinal pigment epithelial (RPE) cells are still unclear. We conducted an in vitro study to investigate the effects of curcumin on aging RPE cells. A pulsed H2O2 exposure aging model was adopted. Aging RPE cells were treated with curcumin 20 µM, 40 µM, and 80 µM. Apoptosis of RPE cells was analyzed by flow cytometry. The intracellular reactive oxygen species concentration was detected using a specific probe and apoptosis-associated proteins were detected by Western blot. Expression of oxidative biomarkers, including superoxide dismutase, maleic dialdehyde, and glutathione, was detected commercially available assay kits. Compared with normal cells, lower cell viability, higher apoptosis rates, and more severe oxidation status were identified in the aging RPE cell model. Curcumin improved cell viability and decreased apoptosis and oxidative stress. Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers. In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells. Accordingly, application of curcumin may be a novel strategy to protect against age-related change in AMD.

No MeSH data available.


Related in: MedlinePlus

Effect of curcumin on ROS content in cultured ARPE-19 cells.Notes: (A) Normal cultured cells used as the control. (B) Aging ARPE-19 cell model used as the positive control. (C) Effect of 20 µM curcumin on ROS content in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on ROS content in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on ROS content in cultured ARPE-19 cells. (F) Summary of ROS content in each group. All studies were conducted in triplicate. ***P<0.001.Abbreviations: CCM, curcumin; ROS, reactive oxygen species.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4590412&req=5

f5-dddt-9-5337: Effect of curcumin on ROS content in cultured ARPE-19 cells.Notes: (A) Normal cultured cells used as the control. (B) Aging ARPE-19 cell model used as the positive control. (C) Effect of 20 µM curcumin on ROS content in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on ROS content in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on ROS content in cultured ARPE-19 cells. (F) Summary of ROS content in each group. All studies were conducted in triplicate. ***P<0.001.Abbreviations: CCM, curcumin; ROS, reactive oxygen species.

Mentions: In general, intracellular ROS concentrations were significantly increased in aging ARPE-19 cells as detected by the specific fluorescent probe used in our study. Compared with normally cultured RPE cells, the aging RPE cells contained higher intracellular ROS levels (6,064.66±73.98 versus 2,585.24±69.39, P<0.001). Intracellular ROS concentrations after treatment with low, middle, and high concentrations of curcumin were 4,919.68±60.38, 3,988.97±48.31, and 3,215.40±55.91, respectively (Figure 5). With increasing concentrations of curcumin, the ROS content decreased accordingly. These findings indicate that treatment with curcumin 20 µM, 40 µM, and 80 µM can effectively prevent age-related production of ROS. Detailed figures and data can be seen in Figure 5.


Effect of curcumin on aging retinal pigment epithelial cells.

Zhu W, Wu Y, Meng YF, Wang JY, Xu M, Tao JJ, Lu J - Drug Des Devel Ther (2015)

Effect of curcumin on ROS content in cultured ARPE-19 cells.Notes: (A) Normal cultured cells used as the control. (B) Aging ARPE-19 cell model used as the positive control. (C) Effect of 20 µM curcumin on ROS content in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on ROS content in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on ROS content in cultured ARPE-19 cells. (F) Summary of ROS content in each group. All studies were conducted in triplicate. ***P<0.001.Abbreviations: CCM, curcumin; ROS, reactive oxygen species.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590412&req=5

f5-dddt-9-5337: Effect of curcumin on ROS content in cultured ARPE-19 cells.Notes: (A) Normal cultured cells used as the control. (B) Aging ARPE-19 cell model used as the positive control. (C) Effect of 20 µM curcumin on ROS content in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on ROS content in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on ROS content in cultured ARPE-19 cells. (F) Summary of ROS content in each group. All studies were conducted in triplicate. ***P<0.001.Abbreviations: CCM, curcumin; ROS, reactive oxygen species.
Mentions: In general, intracellular ROS concentrations were significantly increased in aging ARPE-19 cells as detected by the specific fluorescent probe used in our study. Compared with normally cultured RPE cells, the aging RPE cells contained higher intracellular ROS levels (6,064.66±73.98 versus 2,585.24±69.39, P<0.001). Intracellular ROS concentrations after treatment with low, middle, and high concentrations of curcumin were 4,919.68±60.38, 3,988.97±48.31, and 3,215.40±55.91, respectively (Figure 5). With increasing concentrations of curcumin, the ROS content decreased accordingly. These findings indicate that treatment with curcumin 20 µM, 40 µM, and 80 µM can effectively prevent age-related production of ROS. Detailed figures and data can be seen in Figure 5.

Bottom Line: Curcumin improved cell viability and decreased apoptosis and oxidative stress.Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers.In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Changshu No 2 People's Hospital, Changshu, People's Republic of China.

ABSTRACT
Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population. The antioxidative effects of curcumin on aging retinal pigment epithelial (RPE) cells are still unclear. We conducted an in vitro study to investigate the effects of curcumin on aging RPE cells. A pulsed H2O2 exposure aging model was adopted. Aging RPE cells were treated with curcumin 20 µM, 40 µM, and 80 µM. Apoptosis of RPE cells was analyzed by flow cytometry. The intracellular reactive oxygen species concentration was detected using a specific probe and apoptosis-associated proteins were detected by Western blot. Expression of oxidative biomarkers, including superoxide dismutase, maleic dialdehyde, and glutathione, was detected commercially available assay kits. Compared with normal cells, lower cell viability, higher apoptosis rates, and more severe oxidation status were identified in the aging RPE cell model. Curcumin improved cell viability and decreased apoptosis and oxidative stress. Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers. In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells. Accordingly, application of curcumin may be a novel strategy to protect against age-related change in AMD.

No MeSH data available.


Related in: MedlinePlus