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Effect of curcumin on aging retinal pigment epithelial cells.

Zhu W, Wu Y, Meng YF, Wang JY, Xu M, Tao JJ, Lu J - Drug Des Devel Ther (2015)

Bottom Line: Curcumin improved cell viability and decreased apoptosis and oxidative stress.Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers.In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Changshu No 2 People's Hospital, Changshu, People's Republic of China.

ABSTRACT
Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population. The antioxidative effects of curcumin on aging retinal pigment epithelial (RPE) cells are still unclear. We conducted an in vitro study to investigate the effects of curcumin on aging RPE cells. A pulsed H2O2 exposure aging model was adopted. Aging RPE cells were treated with curcumin 20 µM, 40 µM, and 80 µM. Apoptosis of RPE cells was analyzed by flow cytometry. The intracellular reactive oxygen species concentration was detected using a specific probe and apoptosis-associated proteins were detected by Western blot. Expression of oxidative biomarkers, including superoxide dismutase, maleic dialdehyde, and glutathione, was detected commercially available assay kits. Compared with normal cells, lower cell viability, higher apoptosis rates, and more severe oxidation status were identified in the aging RPE cell model. Curcumin improved cell viability and decreased apoptosis and oxidative stress. Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers. In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells. Accordingly, application of curcumin may be a novel strategy to protect against age-related change in AMD.

No MeSH data available.


Related in: MedlinePlus

Effect of curcumin on apoptosis in cultured ARPE-19 cells.Notes: (A) Normal cultured cells. (B) Aging ARPE-19 cell model. (C) Effect of 20 µM curcumin on apoptosis in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on apoptosis in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on apoptosis in cultured ARPE-19 cells. (F) Summary of early and late apoptosis rates in each group. Differences in comparisons of each dosage of curcumin group and aging group were presented. **P<0.01; ***P<0.001.Abbreviation: CCM, curcumin.
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f3-dddt-9-5337: Effect of curcumin on apoptosis in cultured ARPE-19 cells.Notes: (A) Normal cultured cells. (B) Aging ARPE-19 cell model. (C) Effect of 20 µM curcumin on apoptosis in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on apoptosis in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on apoptosis in cultured ARPE-19 cells. (F) Summary of early and late apoptosis rates in each group. Differences in comparisons of each dosage of curcumin group and aging group were presented. **P<0.01; ***P<0.001.Abbreviation: CCM, curcumin.

Mentions: The aging ARPE-19 cells were considered to be apoptotic, and the rate of apoptosis was analyzed by flow cytometry. In the aging state, increased apoptotic rate was one of the key phenotype of aging change. When ARPE-19 cells were exposed to 800 µM H2O2 in the pulsed-wave mode, there was a significant increase in both early (48.13±1.68 versus 3.93±0.31, P<0.001) and late (1.46±0.29 versus 0.00±0.00, P<0.001) apoptosis when compared with the control group. In the curcumin-treated groups, rates of apoptosis were significantly decreased by all doses given, except for late apoptosis in the 20 µM curcumin group. The improvement in apoptotic state induced by curcumin was also dose-dependent. Augmentation of the curcumin dose was associated with a significant decrease in both early and late apoptotic rates. Further, rates of both early (11.83±1.13) and late (0.00±0.00) apoptosis were significantly decreased in the high-dose curcumin group when compared with the low-dose curcumin group (32.37±0.75, P<0.001). Representative flow cytometry data and detailed results are shown in Figure 3.


Effect of curcumin on aging retinal pigment epithelial cells.

Zhu W, Wu Y, Meng YF, Wang JY, Xu M, Tao JJ, Lu J - Drug Des Devel Ther (2015)

Effect of curcumin on apoptosis in cultured ARPE-19 cells.Notes: (A) Normal cultured cells. (B) Aging ARPE-19 cell model. (C) Effect of 20 µM curcumin on apoptosis in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on apoptosis in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on apoptosis in cultured ARPE-19 cells. (F) Summary of early and late apoptosis rates in each group. Differences in comparisons of each dosage of curcumin group and aging group were presented. **P<0.01; ***P<0.001.Abbreviation: CCM, curcumin.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4590412&req=5

f3-dddt-9-5337: Effect of curcumin on apoptosis in cultured ARPE-19 cells.Notes: (A) Normal cultured cells. (B) Aging ARPE-19 cell model. (C) Effect of 20 µM curcumin on apoptosis in cultured ARPE-19 cells. (D) Effect of 40 µM curcumin on apoptosis in cultured ARPE-19 cells. (E) Effect of 80 µM curcumin on apoptosis in cultured ARPE-19 cells. (F) Summary of early and late apoptosis rates in each group. Differences in comparisons of each dosage of curcumin group and aging group were presented. **P<0.01; ***P<0.001.Abbreviation: CCM, curcumin.
Mentions: The aging ARPE-19 cells were considered to be apoptotic, and the rate of apoptosis was analyzed by flow cytometry. In the aging state, increased apoptotic rate was one of the key phenotype of aging change. When ARPE-19 cells were exposed to 800 µM H2O2 in the pulsed-wave mode, there was a significant increase in both early (48.13±1.68 versus 3.93±0.31, P<0.001) and late (1.46±0.29 versus 0.00±0.00, P<0.001) apoptosis when compared with the control group. In the curcumin-treated groups, rates of apoptosis were significantly decreased by all doses given, except for late apoptosis in the 20 µM curcumin group. The improvement in apoptotic state induced by curcumin was also dose-dependent. Augmentation of the curcumin dose was associated with a significant decrease in both early and late apoptotic rates. Further, rates of both early (11.83±1.13) and late (0.00±0.00) apoptosis were significantly decreased in the high-dose curcumin group when compared with the low-dose curcumin group (32.37±0.75, P<0.001). Representative flow cytometry data and detailed results are shown in Figure 3.

Bottom Line: Curcumin improved cell viability and decreased apoptosis and oxidative stress.Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers.In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Changshu No 2 People's Hospital, Changshu, People's Republic of China.

ABSTRACT
Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population. The antioxidative effects of curcumin on aging retinal pigment epithelial (RPE) cells are still unclear. We conducted an in vitro study to investigate the effects of curcumin on aging RPE cells. A pulsed H2O2 exposure aging model was adopted. Aging RPE cells were treated with curcumin 20 µM, 40 µM, and 80 µM. Apoptosis of RPE cells was analyzed by flow cytometry. The intracellular reactive oxygen species concentration was detected using a specific probe and apoptosis-associated proteins were detected by Western blot. Expression of oxidative biomarkers, including superoxide dismutase, maleic dialdehyde, and glutathione, was detected commercially available assay kits. Compared with normal cells, lower cell viability, higher apoptosis rates, and more severe oxidation status were identified in the aging RPE cell model. Curcumin improved cell viability and decreased apoptosis and oxidative stress. Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers. In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells. Accordingly, application of curcumin may be a novel strategy to protect against age-related change in AMD.

No MeSH data available.


Related in: MedlinePlus