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Effect of curcumin on aging retinal pigment epithelial cells.

Zhu W, Wu Y, Meng YF, Wang JY, Xu M, Tao JJ, Lu J - Drug Des Devel Ther (2015)

Bottom Line: Curcumin improved cell viability and decreased apoptosis and oxidative stress.Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers.In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Changshu No 2 People's Hospital, Changshu, People's Republic of China.

ABSTRACT
Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population. The antioxidative effects of curcumin on aging retinal pigment epithelial (RPE) cells are still unclear. We conducted an in vitro study to investigate the effects of curcumin on aging RPE cells. A pulsed H2O2 exposure aging model was adopted. Aging RPE cells were treated with curcumin 20 µM, 40 µM, and 80 µM. Apoptosis of RPE cells was analyzed by flow cytometry. The intracellular reactive oxygen species concentration was detected using a specific probe and apoptosis-associated proteins were detected by Western blot. Expression of oxidative biomarkers, including superoxide dismutase, maleic dialdehyde, and glutathione, was detected commercially available assay kits. Compared with normal cells, lower cell viability, higher apoptosis rates, and more severe oxidation status were identified in the aging RPE cell model. Curcumin improved cell viability and decreased apoptosis and oxidative stress. Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers. In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells. Accordingly, application of curcumin may be a novel strategy to protect against age-related change in AMD.

No MeSH data available.


Related in: MedlinePlus

Effect of curcumin on viability of cultured ARPE-19 cells.Notes: (A) Effect on the normal cultured ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM and (B) effect on aging ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM. **P<0.01; ***P<0.001.
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f2-dddt-9-5337: Effect of curcumin on viability of cultured ARPE-19 cells.Notes: (A) Effect on the normal cultured ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM and (B) effect on aging ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM. **P<0.01; ***P<0.001.

Mentions: We conducted a series of studies to demonstrate the effect of curcumin on ARPE-19 cell viability in both the normal and senescent states. Cell viability was measured by MTT assay. In this in vitro experiment, curcumin had no significant effect on the viability of cells under normal conditions (Figure 2A). Curcumin 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM had no significant protective effect when compared with the control group. However, in the aging state, curcumin significantly improved cell viability when compared with the control group. As showed in Figure 2B, the protective effect of curcumin on aging RPE cells was dose-dependent.


Effect of curcumin on aging retinal pigment epithelial cells.

Zhu W, Wu Y, Meng YF, Wang JY, Xu M, Tao JJ, Lu J - Drug Des Devel Ther (2015)

Effect of curcumin on viability of cultured ARPE-19 cells.Notes: (A) Effect on the normal cultured ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM and (B) effect on aging ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM. **P<0.01; ***P<0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590412&req=5

f2-dddt-9-5337: Effect of curcumin on viability of cultured ARPE-19 cells.Notes: (A) Effect on the normal cultured ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM and (B) effect on aging ARPE-19 cells at doses of 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM. **P<0.01; ***P<0.001.
Mentions: We conducted a series of studies to demonstrate the effect of curcumin on ARPE-19 cell viability in both the normal and senescent states. Cell viability was measured by MTT assay. In this in vitro experiment, curcumin had no significant effect on the viability of cells under normal conditions (Figure 2A). Curcumin 10 µM, 20 µM, 40 µM, 60 µM, 80 µM, and 100 µM had no significant protective effect when compared with the control group. However, in the aging state, curcumin significantly improved cell viability when compared with the control group. As showed in Figure 2B, the protective effect of curcumin on aging RPE cells was dose-dependent.

Bottom Line: Curcumin improved cell viability and decreased apoptosis and oxidative stress.Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers.In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Changshu No 2 People's Hospital, Changshu, People's Republic of China.

ABSTRACT
Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population. The antioxidative effects of curcumin on aging retinal pigment epithelial (RPE) cells are still unclear. We conducted an in vitro study to investigate the effects of curcumin on aging RPE cells. A pulsed H2O2 exposure aging model was adopted. Aging RPE cells were treated with curcumin 20 µM, 40 µM, and 80 µM. Apoptosis of RPE cells was analyzed by flow cytometry. The intracellular reactive oxygen species concentration was detected using a specific probe and apoptosis-associated proteins were detected by Western blot. Expression of oxidative biomarkers, including superoxide dismutase, maleic dialdehyde, and glutathione, was detected commercially available assay kits. Compared with normal cells, lower cell viability, higher apoptosis rates, and more severe oxidation status were identified in the aging RPE cell model. Curcumin improved cell viability and decreased apoptosis and oxidative stress. Further, curcumin had a significant influence on expression of apoptosis-associated proteins and oxidative stress biomarkers. In conclusion, treatment with curcumin was able to regulate proliferation, oxidative stress, and apoptosis in aging RPE cells. Accordingly, application of curcumin may be a novel strategy to protect against age-related change in AMD.

No MeSH data available.


Related in: MedlinePlus