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Influenza and respiratory syncytial viruses: Efficacy of different diagnostic assays.

Rahman MM, Wong KK, Alfizah H, Hussin S, Isahak I - Pak J Med Sci (2015 Jul-Aug)

Bottom Line: Total 2781 specimens of throat swabs and nasopharyngeal aspirates were obtained from patients suspected of respiratory viruses' infections from January 2009 to December 2011 at Universiti Kebangsaan Malaysia Medical Centre(UKMMC).On the other hand, rRT-PCR was able to identify 189 of 505 (37.43%) specimens in which 65 were influenza A virus and 124 were RSV.These advantages help in managing patients by saving cost and hospitalization stay.

View Article: PubMed Central - PubMed

Affiliation: M. M. Rahman, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.

ABSTRACT

Objective: To determine the efficacy of cell culture, immunoflourescence Assay (IFA) and real time polymerase chain reaction (rRT-PCR) in relation to diagnosis of influenza and Respiratory Syncytial Virus (RSV).

Methods: Total 2781 specimens of throat swabs and nasopharyngeal aspirates were obtained from patients suspected of respiratory viruses' infections from January 2009 to December 2011 at Universiti Kebangsaan Malaysia Medical Centre(UKMMC). The specimens were processed by cell culture and immunoflurescence assay (IFA) and (rRT-PCR).

Results: Thirty three (1.19%) specimens were positive for influenza virus A and 42 (1.51%) were positive for RSV by cell culture and IFA. On the other hand, rRT-PCR was able to identify 189 of 505 (37.43%) specimens in which 65 were influenza A virus and 124 were RSV. Sensitivity of rRT-PCR was 100% for both influenza A virus and RSV and specificity was 88% and 77% for influenza A virus and RSV, respectively.

Conclusion: rRT-PCR diagnosed respiratory viruses in shorter time with a high level of sensitivity in comparison to conventional assays - cell culture and IFA. These advantages help in managing patients by saving cost and hospitalization stay.

No MeSH data available.


Related in: MedlinePlus

Number of positive isolates of influenza A virus and RSV detected by cell culture and Immunofluorescence Assay and rRT-PCR.
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Figure 3: Number of positive isolates of influenza A virus and RSV detected by cell culture and Immunofluorescence Assay and rRT-PCR.

Mentions: The 12 specimens’ positive for influenza A virus and RSV by conventional virus isolation technique were shown to be positive by rRT-PCR assay too. In addition, rRT-PCR detected positive an additional 177 specimens that included 115 cases of RSV and 62 cases of influenza A virus. The positivity of rRT-PCR assay for influenza A virus was 12.9% (65/505) and for RSV it was 24.6% (124/505). It was proved that rRT-PCR is more efficient than conventional method of virus isolation (Fig.3) Sensitivity of the in-house developed rRT-PCR was 100% for both influenza A virus and RSV and specificity was 88% and 77% for influenza A virus and RSV, respectively.


Influenza and respiratory syncytial viruses: Efficacy of different diagnostic assays.

Rahman MM, Wong KK, Alfizah H, Hussin S, Isahak I - Pak J Med Sci (2015 Jul-Aug)

Number of positive isolates of influenza A virus and RSV detected by cell culture and Immunofluorescence Assay and rRT-PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590406&req=5

Figure 3: Number of positive isolates of influenza A virus and RSV detected by cell culture and Immunofluorescence Assay and rRT-PCR.
Mentions: The 12 specimens’ positive for influenza A virus and RSV by conventional virus isolation technique were shown to be positive by rRT-PCR assay too. In addition, rRT-PCR detected positive an additional 177 specimens that included 115 cases of RSV and 62 cases of influenza A virus. The positivity of rRT-PCR assay for influenza A virus was 12.9% (65/505) and for RSV it was 24.6% (124/505). It was proved that rRT-PCR is more efficient than conventional method of virus isolation (Fig.3) Sensitivity of the in-house developed rRT-PCR was 100% for both influenza A virus and RSV and specificity was 88% and 77% for influenza A virus and RSV, respectively.

Bottom Line: Total 2781 specimens of throat swabs and nasopharyngeal aspirates were obtained from patients suspected of respiratory viruses' infections from January 2009 to December 2011 at Universiti Kebangsaan Malaysia Medical Centre(UKMMC).On the other hand, rRT-PCR was able to identify 189 of 505 (37.43%) specimens in which 65 were influenza A virus and 124 were RSV.These advantages help in managing patients by saving cost and hospitalization stay.

View Article: PubMed Central - PubMed

Affiliation: M. M. Rahman, Department of Medical Microbiology & Immunology, Faculty of Medicine, The National University of Malaysia, Cheras, 56000, Kuala Lumpur, Malaysia.

ABSTRACT

Objective: To determine the efficacy of cell culture, immunoflourescence Assay (IFA) and real time polymerase chain reaction (rRT-PCR) in relation to diagnosis of influenza and Respiratory Syncytial Virus (RSV).

Methods: Total 2781 specimens of throat swabs and nasopharyngeal aspirates were obtained from patients suspected of respiratory viruses' infections from January 2009 to December 2011 at Universiti Kebangsaan Malaysia Medical Centre(UKMMC). The specimens were processed by cell culture and immunoflurescence assay (IFA) and (rRT-PCR).

Results: Thirty three (1.19%) specimens were positive for influenza virus A and 42 (1.51%) were positive for RSV by cell culture and IFA. On the other hand, rRT-PCR was able to identify 189 of 505 (37.43%) specimens in which 65 were influenza A virus and 124 were RSV. Sensitivity of rRT-PCR was 100% for both influenza A virus and RSV and specificity was 88% and 77% for influenza A virus and RSV, respectively.

Conclusion: rRT-PCR diagnosed respiratory viruses in shorter time with a high level of sensitivity in comparison to conventional assays - cell culture and IFA. These advantages help in managing patients by saving cost and hospitalization stay.

No MeSH data available.


Related in: MedlinePlus