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Cytogenetic and Molecular Analyses of Philadelphia Chromosome Variants in CML (chronic myeloid leukemia) Patients from Sindh using Karyotyping and RT-PCR.

Ujjan ID, Akhund AA, Saboor M, Qureshi MA, Khan S - Pak J Med Sci (2015 Jul-Aug)

Bottom Line: Frequency of the Ph was found to be of 90.9% in CML patients using a highly sensitive technique, the RT-PCR.Cytogenetic and molecular studies must be conducted for better management of CML cases.These findings could be very useful in guiding the appropriate therapeutic options for CML patients.

View Article: PubMed Central - PubMed

Affiliation: Dr. Ikram Din Ujjan, PhD. Department of Pathology, Liaquat University of Medical and Health Sciences, Hyderabad, Pakistan.

ABSTRACT

Objective: To determine the frequency of Philadelphia chromosome (Ph) and its variants in chronic myeloid leukemia (CML) cases at a tertiary care hospital of Sindh.

Methods: The study was conducted at the Department of Pathology, Liaquat University of Medical and Health Sciences, Jamshoro and Isra University Hospital, Hyderabad during May-to-September 2014. Bone marrow and peripheral blood samples from a total of 145 diagnosed cases of CML were collected. Cytogenetic analyses were performed using karyotyping as per the International System for Human Cytogenetic Nomenclature guidelines. All karyotypic images were analyzed using the Cytovision software. In order to identify BCR-ABL transcripts, RT-PCR was performed. Statistical analysis of the data was done using SPSS-version-21.0.

Results: Of the 145 samples, a total of 133 (91.7%) were positive for the Ph (Ph+) while 12 (8.3%) were negative for the Ph (Ph-). Of the 133 Ph+ samples, standard karyotypes were noted in 121 (91%), simple variants in 9 (6.7%) and complex variants in 3 (2.3%) of the samples. All the Ph+ samples (n=133) showed BCR-ABL positivity. Of the 12 Ph- samples, a total of 7 (58.3%) were BCR-ABL-positive and 5 (41.6%) were BCR-ABL-negative.

Conclusion: Frequency of the Ph was found to be of 90.9% in CML patients using a highly sensitive technique, the RT-PCR. Cytogenetic abnormalities were at a lower frequency. Cytogenetic and molecular studies must be conducted for better management of CML cases. These findings could be very useful in guiding the appropriate therapeutic options for CML patients.

No MeSH data available.


Related in: MedlinePlus

(A–B): Chronic myeloid leukemia (CML) showing hypercellular bone marrow on H & E staining at ×100 and ×400 magnification. (C): Classical karyotype of CML translocation – t(9; 22) & (D): CML Complex translocation 9,6,19,22.
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Figure 1: (A–B): Chronic myeloid leukemia (CML) showing hypercellular bone marrow on H & E staining at ×100 and ×400 magnification. (C): Classical karyotype of CML translocation – t(9; 22) & (D): CML Complex translocation 9,6,19,22.

Mentions: Of the 145 patients, Ph+ chromosome was noted in 133 (91.7%) CML patients. Of the 133 Ph+ chromosome, standard chromosome was noted in 121 (90.9%), simple variant in 9 (6.7%) and complex variants were noted in 3 (2.2%) of cases (Table-III and Fig.1). All Ph+ patients showed BCR-ABL positivity. Of the 12 Ph- samples, 7 were BCR-ABL positive and 5 were BCR-ABL negative.


Cytogenetic and Molecular Analyses of Philadelphia Chromosome Variants in CML (chronic myeloid leukemia) Patients from Sindh using Karyotyping and RT-PCR.

Ujjan ID, Akhund AA, Saboor M, Qureshi MA, Khan S - Pak J Med Sci (2015 Jul-Aug)

(A–B): Chronic myeloid leukemia (CML) showing hypercellular bone marrow on H & E staining at ×100 and ×400 magnification. (C): Classical karyotype of CML translocation – t(9; 22) & (D): CML Complex translocation 9,6,19,22.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590377&req=5

Figure 1: (A–B): Chronic myeloid leukemia (CML) showing hypercellular bone marrow on H & E staining at ×100 and ×400 magnification. (C): Classical karyotype of CML translocation – t(9; 22) & (D): CML Complex translocation 9,6,19,22.
Mentions: Of the 145 patients, Ph+ chromosome was noted in 133 (91.7%) CML patients. Of the 133 Ph+ chromosome, standard chromosome was noted in 121 (90.9%), simple variant in 9 (6.7%) and complex variants were noted in 3 (2.2%) of cases (Table-III and Fig.1). All Ph+ patients showed BCR-ABL positivity. Of the 12 Ph- samples, 7 were BCR-ABL positive and 5 were BCR-ABL negative.

Bottom Line: Frequency of the Ph was found to be of 90.9% in CML patients using a highly sensitive technique, the RT-PCR.Cytogenetic and molecular studies must be conducted for better management of CML cases.These findings could be very useful in guiding the appropriate therapeutic options for CML patients.

View Article: PubMed Central - PubMed

Affiliation: Dr. Ikram Din Ujjan, PhD. Department of Pathology, Liaquat University of Medical and Health Sciences, Hyderabad, Pakistan.

ABSTRACT

Objective: To determine the frequency of Philadelphia chromosome (Ph) and its variants in chronic myeloid leukemia (CML) cases at a tertiary care hospital of Sindh.

Methods: The study was conducted at the Department of Pathology, Liaquat University of Medical and Health Sciences, Jamshoro and Isra University Hospital, Hyderabad during May-to-September 2014. Bone marrow and peripheral blood samples from a total of 145 diagnosed cases of CML were collected. Cytogenetic analyses were performed using karyotyping as per the International System for Human Cytogenetic Nomenclature guidelines. All karyotypic images were analyzed using the Cytovision software. In order to identify BCR-ABL transcripts, RT-PCR was performed. Statistical analysis of the data was done using SPSS-version-21.0.

Results: Of the 145 samples, a total of 133 (91.7%) were positive for the Ph (Ph+) while 12 (8.3%) were negative for the Ph (Ph-). Of the 133 Ph+ samples, standard karyotypes were noted in 121 (91%), simple variants in 9 (6.7%) and complex variants in 3 (2.3%) of the samples. All the Ph+ samples (n=133) showed BCR-ABL positivity. Of the 12 Ph- samples, a total of 7 (58.3%) were BCR-ABL-positive and 5 (41.6%) were BCR-ABL-negative.

Conclusion: Frequency of the Ph was found to be of 90.9% in CML patients using a highly sensitive technique, the RT-PCR. Cytogenetic abnormalities were at a lower frequency. Cytogenetic and molecular studies must be conducted for better management of CML cases. These findings could be very useful in guiding the appropriate therapeutic options for CML patients.

No MeSH data available.


Related in: MedlinePlus