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Collagenase IV plays an important role in regulating hair cycle by inducing VEGF, IGF-1, and TGF-β expression.

Hou C, Miao Y, Wang J, Wang X, Chen CY, Hu ZQ - Drug Des Devel Ther (2015)

Bottom Line: We found mRNA and protein expressions of VEGF and IGF-1 fluctuated with a peak at anagen and a decrease at catagen to telogen.The MMPs agonist also significantly increased expression of VEGF, IGF-1, and TGF-β.Meanwhile, SB-3CT treatment significantly suppressed hair growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT

Background: It has been reported that collagenases (matrix metalloproteinase 2 [MMP-2] and matrix metalloproteinase 9 [MMP-9]) are associated with hair cycle, whereas the mechanism of the association is largely unknown.

Methods: The mice were randomly allocated into four groups: saline, and 5, 10, and 15 nM SB-3CT. Immunohistochemical analysis was employed to examine MMP-2 and MMP-9 protein. Real-time polymerase chain reaction and enzyme-linked immunosorbent assay were performed to determine mRNA and protein levels of VEGF, IGF-1, TGF-β, and GAPDH. Growing hair follicles from anagen phase III-IV were scored based on hematoxylin and eosin staining. Hair regrowth was also evaluated.

Results: Results showed that mRNA expressions of enzymes changed with a peak at late anagen and a trough at telogen after depilation. Immunostaining showed that the highest expression of MMP-2 was more than that of MMP-9, and the highest expression of enzymes changed during anagen. The localizations of MMP-2 changed from dermal papilla, keratinocyte strand, out of root sheath, and basal plate at early anagen, to hair bulb, inner root sheath, and outer root sheath at late anagen. The localization of MMP-9 changed from partial keratinocyte to dermal papilla at early anagen and to outer root sheath at late anagen. VEGF, IGF-1, and TGF-β have been shown to regulate hair growth. We found mRNA and protein expressions of VEGF and IGF-1 fluctuated with a peak at anagen and a decrease at catagen to telogen. In contrast, mRNA and protein expressions of TGF-β changed with highest and lowest levels at anagen and telogen, respectively. With selective inhibitor of collagenase IV, SB-3CT, mice showed significant suppressed hair growth and decreased expression of VEGF, IGF-1, and TGF-β. The MMPs agonist also significantly increased expression of VEGF, IGF-1, and TGF-β. Meanwhile, SB-3CT treatment significantly suppressed hair growth.

Conclusion: All these data suggest that the type IV collagenases, MMP-2 and MMP-9, play important roles in hair cycle, and this could be mediated by induced expression of VEGF, IGF-1, and TGF-β.

No MeSH data available.


Effect of topical application of the collagenase IV inhibitor, SB-3CT, on hair regrowth.Notes: After 5, 15, and 25 days treatment, typical photos of dorsal skin treated with vehicle (A–C), and SB-3CT at 5 nM (D–F), 10 nM (G–I) and 20 nM (J–L). Upper row is from the mice 5 days after application, middle row 15 days, and bottom row 25 days.
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f3-dddt-9-5373: Effect of topical application of the collagenase IV inhibitor, SB-3CT, on hair regrowth.Notes: After 5, 15, and 25 days treatment, typical photos of dorsal skin treated with vehicle (A–C), and SB-3CT at 5 nM (D–F), 10 nM (G–I) and 20 nM (J–L). Upper row is from the mice 5 days after application, middle row 15 days, and bottom row 25 days.

Mentions: To evaluate the effect of SB-3CT on hair growth, typical photos of the dorsal skin in mice at 0, 5, 15, and 25 days after depilation were taken. As seen in Figure 3, the mice treated with 5, 10, and 15 nM SB-3CT showed dose-dependent inhibition of hair recovery compared to the control group. At 25 days after depilation, SB-3CT-treated mice showed hair regrowth of about 90%, 80%, and 52% at 5, 10, and 15 nM, respectively, whereas vehicle treated mice showed about 93% (Figure 3). Although the inhibitory effect on hair regrowth was most significant at 15 nM (Figures 3 and 4), the mice showed slightly decreased activity during the early phase of treatment. Therefore, 10 nM SB-3CT was selected for the following study.


Collagenase IV plays an important role in regulating hair cycle by inducing VEGF, IGF-1, and TGF-β expression.

Hou C, Miao Y, Wang J, Wang X, Chen CY, Hu ZQ - Drug Des Devel Ther (2015)

Effect of topical application of the collagenase IV inhibitor, SB-3CT, on hair regrowth.Notes: After 5, 15, and 25 days treatment, typical photos of dorsal skin treated with vehicle (A–C), and SB-3CT at 5 nM (D–F), 10 nM (G–I) and 20 nM (J–L). Upper row is from the mice 5 days after application, middle row 15 days, and bottom row 25 days.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590320&req=5

f3-dddt-9-5373: Effect of topical application of the collagenase IV inhibitor, SB-3CT, on hair regrowth.Notes: After 5, 15, and 25 days treatment, typical photos of dorsal skin treated with vehicle (A–C), and SB-3CT at 5 nM (D–F), 10 nM (G–I) and 20 nM (J–L). Upper row is from the mice 5 days after application, middle row 15 days, and bottom row 25 days.
Mentions: To evaluate the effect of SB-3CT on hair growth, typical photos of the dorsal skin in mice at 0, 5, 15, and 25 days after depilation were taken. As seen in Figure 3, the mice treated with 5, 10, and 15 nM SB-3CT showed dose-dependent inhibition of hair recovery compared to the control group. At 25 days after depilation, SB-3CT-treated mice showed hair regrowth of about 90%, 80%, and 52% at 5, 10, and 15 nM, respectively, whereas vehicle treated mice showed about 93% (Figure 3). Although the inhibitory effect on hair regrowth was most significant at 15 nM (Figures 3 and 4), the mice showed slightly decreased activity during the early phase of treatment. Therefore, 10 nM SB-3CT was selected for the following study.

Bottom Line: We found mRNA and protein expressions of VEGF and IGF-1 fluctuated with a peak at anagen and a decrease at catagen to telogen.The MMPs agonist also significantly increased expression of VEGF, IGF-1, and TGF-β.Meanwhile, SB-3CT treatment significantly suppressed hair growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT

Background: It has been reported that collagenases (matrix metalloproteinase 2 [MMP-2] and matrix metalloproteinase 9 [MMP-9]) are associated with hair cycle, whereas the mechanism of the association is largely unknown.

Methods: The mice were randomly allocated into four groups: saline, and 5, 10, and 15 nM SB-3CT. Immunohistochemical analysis was employed to examine MMP-2 and MMP-9 protein. Real-time polymerase chain reaction and enzyme-linked immunosorbent assay were performed to determine mRNA and protein levels of VEGF, IGF-1, TGF-β, and GAPDH. Growing hair follicles from anagen phase III-IV were scored based on hematoxylin and eosin staining. Hair regrowth was also evaluated.

Results: Results showed that mRNA expressions of enzymes changed with a peak at late anagen and a trough at telogen after depilation. Immunostaining showed that the highest expression of MMP-2 was more than that of MMP-9, and the highest expression of enzymes changed during anagen. The localizations of MMP-2 changed from dermal papilla, keratinocyte strand, out of root sheath, and basal plate at early anagen, to hair bulb, inner root sheath, and outer root sheath at late anagen. The localization of MMP-9 changed from partial keratinocyte to dermal papilla at early anagen and to outer root sheath at late anagen. VEGF, IGF-1, and TGF-β have been shown to regulate hair growth. We found mRNA and protein expressions of VEGF and IGF-1 fluctuated with a peak at anagen and a decrease at catagen to telogen. In contrast, mRNA and protein expressions of TGF-β changed with highest and lowest levels at anagen and telogen, respectively. With selective inhibitor of collagenase IV, SB-3CT, mice showed significant suppressed hair growth and decreased expression of VEGF, IGF-1, and TGF-β. The MMPs agonist also significantly increased expression of VEGF, IGF-1, and TGF-β. Meanwhile, SB-3CT treatment significantly suppressed hair growth.

Conclusion: All these data suggest that the type IV collagenases, MMP-2 and MMP-9, play important roles in hair cycle, and this could be mediated by induced expression of VEGF, IGF-1, and TGF-β.

No MeSH data available.