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Inhibition of tyrosinase activity and melanine pigmentation by 2-hydroxytyrosol.

Uchida R, Ishikawa S, Tomoda H - Acta Pharm Sin B (2014)

Bottom Line: 2-Hydroxytyrosol (2-HT), originally reported as a synthetic compound, was isolated for the first time as a fungal metabolite. 2-HT was found to inhibit mushroom tyrosinase with an IC50 value of 13.0 µmol/L.Furthermore, 2-HT dose-dependently inhibited tyrosinase activity (IC50, 32.5 µmol/L) in the cell-free extract of B16 melanoma cells and α-melanocyte stimulating hormone (α-MSH)-stimulated melanin formation in intact B16 melanoma cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo 108-8641, Japan ; School of Pharmacy, Kitasato University, Tokyo 108-8641, Japan.

ABSTRACT
2-Hydroxytyrosol (2-HT), originally reported as a synthetic compound, was isolated for the first time as a fungal metabolite. 2-HT was found to inhibit mushroom tyrosinase with an IC50 value of 13.0 µmol/L. Furthermore, 2-HT dose-dependently inhibited tyrosinase activity (IC50, 32.5 µmol/L) in the cell-free extract of B16 melanoma cells and α-melanocyte stimulating hormone (α-MSH)-stimulated melanin formation in intact B16 melanoma cells.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effects of 2-HT(●) and kojic acid (■) against mushroom tyrosinase.
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f0010: Inhibitory effects of 2-HT(●) and kojic acid (■) against mushroom tyrosinase.

Mentions: In this assay, the conversion of l-DOPA to dopaquinone by mushroom tyrosinase was observed at 450 nm. As shown in Fig. 2, 2-HT dose-dependently inhibited mushroom tyrosinase activity with an IC50 value of 13.0 µmol/L. Under the same conditions, kojic acid also inhibited the activity with IC50 of 14.8 µmol/L.


Inhibition of tyrosinase activity and melanine pigmentation by 2-hydroxytyrosol.

Uchida R, Ishikawa S, Tomoda H - Acta Pharm Sin B (2014)

Inhibitory effects of 2-HT(●) and kojic acid (■) against mushroom tyrosinase.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590298&req=5

f0010: Inhibitory effects of 2-HT(●) and kojic acid (■) against mushroom tyrosinase.
Mentions: In this assay, the conversion of l-DOPA to dopaquinone by mushroom tyrosinase was observed at 450 nm. As shown in Fig. 2, 2-HT dose-dependently inhibited mushroom tyrosinase activity with an IC50 value of 13.0 µmol/L. Under the same conditions, kojic acid also inhibited the activity with IC50 of 14.8 µmol/L.

Bottom Line: 2-Hydroxytyrosol (2-HT), originally reported as a synthetic compound, was isolated for the first time as a fungal metabolite. 2-HT was found to inhibit mushroom tyrosinase with an IC50 value of 13.0 µmol/L.Furthermore, 2-HT dose-dependently inhibited tyrosinase activity (IC50, 32.5 µmol/L) in the cell-free extract of B16 melanoma cells and α-melanocyte stimulating hormone (α-MSH)-stimulated melanin formation in intact B16 melanoma cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo 108-8641, Japan ; School of Pharmacy, Kitasato University, Tokyo 108-8641, Japan.

ABSTRACT
2-Hydroxytyrosol (2-HT), originally reported as a synthetic compound, was isolated for the first time as a fungal metabolite. 2-HT was found to inhibit mushroom tyrosinase with an IC50 value of 13.0 µmol/L. Furthermore, 2-HT dose-dependently inhibited tyrosinase activity (IC50, 32.5 µmol/L) in the cell-free extract of B16 melanoma cells and α-melanocyte stimulating hormone (α-MSH)-stimulated melanin formation in intact B16 melanoma cells.

No MeSH data available.


Related in: MedlinePlus