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High genetic abundance of Rpi-blb2/Mi-1.2/Cami gene family in Solanaceae.

Zhao L, Zhang Q, Gao R, Yang S, Liu H, Zhang X - BMC Evol. Biol. (2015)

Bottom Line: This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species.Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest.Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210000, China. swjszln0524@163.com.

ABSTRACT

Background: Three NBS-LRR genes, Rpi-blb2, Mi-1.2, and Cami, constitute a very special plant resistance gene family. These genes confer resistance against 4 distantly related pathogen species in 3 different Solanaceae hosts. To characterize this noted resistance, we conducted a series of studies on this gene family.

Results: First, homologs of this gene family were identified in the pepper, tomato and potato genomes. This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species. To gain more information pertaining to gene resistance within this family, 121 LRR regions were cloned in 16 different wild/cultivated potato accessions. Again, frequent copy number variations and a high level of divergence between homolog were observed common among accessions. The divergence within species was so high that it reaches the level of divergence between species. Also, frequent frameshift mutations and abundant gene conversion events were identified in these LRR regions.

Conclusions: Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest. Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

Show MeSH
Phylogenetic tree derived from the 121 LRR regions cloned in this study and 28 LRR domains from previous studies. The 121 LRR domains were cloned from 16 different potato accessions/genotypes, 23 of the 28 LRR domains were drawn from the potato genomes and the rest 5 from previous cloned genes in potato, pepper, and tomato
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Fig3: Phylogenetic tree derived from the 121 LRR regions cloned in this study and 28 LRR domains from previous studies. The 121 LRR domains were cloned from 16 different potato accessions/genotypes, 23 of the 28 LRR domains were drawn from the potato genomes and the rest 5 from previous cloned genes in potato, pepper, and tomato

Mentions: To investigate the genetic relations and their evolutionary history between LRR homologs from different accessions, a phylogenetic tree was constructed based on cloned LRR regions, together with LRR sequences from potato genome and the functional genes (Fig. 3). The topological structure of the tree showed the cloned LRR regions to be scattered, suggesting that the LRR regions had a high degree of polymorphisms and varied between accessions and within accessions. Moreover, the LRR regions were not distinctly divided into two clear groups, wild and cultivated clades (Fig. 3), indicating a frequent introgression during the cultivation of potato.Fig. 3


High genetic abundance of Rpi-blb2/Mi-1.2/Cami gene family in Solanaceae.

Zhao L, Zhang Q, Gao R, Yang S, Liu H, Zhang X - BMC Evol. Biol. (2015)

Phylogenetic tree derived from the 121 LRR regions cloned in this study and 28 LRR domains from previous studies. The 121 LRR domains were cloned from 16 different potato accessions/genotypes, 23 of the 28 LRR domains were drawn from the potato genomes and the rest 5 from previous cloned genes in potato, pepper, and tomato
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4590265&req=5

Fig3: Phylogenetic tree derived from the 121 LRR regions cloned in this study and 28 LRR domains from previous studies. The 121 LRR domains were cloned from 16 different potato accessions/genotypes, 23 of the 28 LRR domains were drawn from the potato genomes and the rest 5 from previous cloned genes in potato, pepper, and tomato
Mentions: To investigate the genetic relations and their evolutionary history between LRR homologs from different accessions, a phylogenetic tree was constructed based on cloned LRR regions, together with LRR sequences from potato genome and the functional genes (Fig. 3). The topological structure of the tree showed the cloned LRR regions to be scattered, suggesting that the LRR regions had a high degree of polymorphisms and varied between accessions and within accessions. Moreover, the LRR regions were not distinctly divided into two clear groups, wild and cultivated clades (Fig. 3), indicating a frequent introgression during the cultivation of potato.Fig. 3

Bottom Line: This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species.Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest.Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210000, China. swjszln0524@163.com.

ABSTRACT

Background: Three NBS-LRR genes, Rpi-blb2, Mi-1.2, and Cami, constitute a very special plant resistance gene family. These genes confer resistance against 4 distantly related pathogen species in 3 different Solanaceae hosts. To characterize this noted resistance, we conducted a series of studies on this gene family.

Results: First, homologs of this gene family were identified in the pepper, tomato and potato genomes. This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species. To gain more information pertaining to gene resistance within this family, 121 LRR regions were cloned in 16 different wild/cultivated potato accessions. Again, frequent copy number variations and a high level of divergence between homolog were observed common among accessions. The divergence within species was so high that it reaches the level of divergence between species. Also, frequent frameshift mutations and abundant gene conversion events were identified in these LRR regions.

Conclusions: Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest. Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

Show MeSH