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High genetic abundance of Rpi-blb2/Mi-1.2/Cami gene family in Solanaceae.

Zhao L, Zhang Q, Gao R, Yang S, Liu H, Zhang X - BMC Evol. Biol. (2015)

Bottom Line: This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species.Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest.Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210000, China. swjszln0524@163.com.

ABSTRACT

Background: Three NBS-LRR genes, Rpi-blb2, Mi-1.2, and Cami, constitute a very special plant resistance gene family. These genes confer resistance against 4 distantly related pathogen species in 3 different Solanaceae hosts. To characterize this noted resistance, we conducted a series of studies on this gene family.

Results: First, homologs of this gene family were identified in the pepper, tomato and potato genomes. This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species. To gain more information pertaining to gene resistance within this family, 121 LRR regions were cloned in 16 different wild/cultivated potato accessions. Again, frequent copy number variations and a high level of divergence between homolog were observed common among accessions. The divergence within species was so high that it reaches the level of divergence between species. Also, frequent frameshift mutations and abundant gene conversion events were identified in these LRR regions.

Conclusions: Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest. Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

Show MeSH
Phylogenetic tree derived from Rpi-blb2/Mi-1.2/Cami homologs. The maximum-likelihood phylogenetic tree is constructed by PhyML 3.1 based on the CDS sequences of each homolog
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Fig2: Phylogenetic tree derived from Rpi-blb2/Mi-1.2/Cami homologs. The maximum-likelihood phylogenetic tree is constructed by PhyML 3.1 based on the CDS sequences of each homolog

Mentions: To further explore the evolutionary history of this gene family, a phylogenetic tree was constructed based on the cloned functional genes and their homologs within the 3 reference genomes. As shown in Fig. 2, genes from the same species mostly clustered together on the tree. This kind of topology further indicates that the expansion of this gene family took place separately in these 3 species after they split from their common ancestor. This tree also shows a closer homology between the tomato and potato than with pepper, coinciding with their genetic relationships on the species level.Fig. 2


High genetic abundance of Rpi-blb2/Mi-1.2/Cami gene family in Solanaceae.

Zhao L, Zhang Q, Gao R, Yang S, Liu H, Zhang X - BMC Evol. Biol. (2015)

Phylogenetic tree derived from Rpi-blb2/Mi-1.2/Cami homologs. The maximum-likelihood phylogenetic tree is constructed by PhyML 3.1 based on the CDS sequences of each homolog
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4590265&req=5

Fig2: Phylogenetic tree derived from Rpi-blb2/Mi-1.2/Cami homologs. The maximum-likelihood phylogenetic tree is constructed by PhyML 3.1 based on the CDS sequences of each homolog
Mentions: To further explore the evolutionary history of this gene family, a phylogenetic tree was constructed based on the cloned functional genes and their homologs within the 3 reference genomes. As shown in Fig. 2, genes from the same species mostly clustered together on the tree. This kind of topology further indicates that the expansion of this gene family took place separately in these 3 species after they split from their common ancestor. This tree also shows a closer homology between the tomato and potato than with pepper, coinciding with their genetic relationships on the species level.Fig. 2

Bottom Line: This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species.Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest.Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210000, China. swjszln0524@163.com.

ABSTRACT

Background: Three NBS-LRR genes, Rpi-blb2, Mi-1.2, and Cami, constitute a very special plant resistance gene family. These genes confer resistance against 4 distantly related pathogen species in 3 different Solanaceae hosts. To characterize this noted resistance, we conducted a series of studies on this gene family.

Results: First, homologs of this gene family were identified in the pepper, tomato and potato genomes. This revealed a large variation in copy number within this gene family among species and a great divergence was found both between and within species. To gain more information pertaining to gene resistance within this family, 121 LRR regions were cloned in 16 different wild/cultivated potato accessions. Again, frequent copy number variations and a high level of divergence between homolog were observed common among accessions. The divergence within species was so high that it reaches the level of divergence between species. Also, frequent frameshift mutations and abundant gene conversion events were identified in these LRR regions.

Conclusions: Our findings suggest that this family harbors an unusually high level of genetic abundance, making it of particular interest. Together with other reported examples, our study also provides evidence that multi-resistance is a common trait in R gene families like this.

Show MeSH