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Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury.

Zhao H, Yang BL, Liu ZX, Yu Q, Zhang WJ, Yuan K, Zeng HH, Zhu GC, Liu DM, Li Q - Neural Regen Res (2015)

Bottom Line: However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear.In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury.Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Basic Medical School, Nanchang University, Nanchang, Jiangxi Province, China ; Medical Department, Graduate School, Nanchang University, Nanchang, Jiangxi Province, China.

ABSTRACT
Olfactory bulb tissue transplantation inhibits P2X2/3 receptor-mediated neuropathic pain. However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear. In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury. We assessed mechanical nociception in the rat models 7 and 14 days after surgery by measuring paw withdrawal threshold, and examined P2X2/3 receptor expression in L4-5 dorsal root ganglia using immunohistochemistry. Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia. At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals. Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

No MeSH data available.


Related in: MedlinePlus

P2X3 receptor immunoreactivity in rat L4–5 dorsal root ganglia.Immunoreactive products of the P2X3 receptor (yellow-brown, arrows), observed mainly in the cytoplasm, 7 and 14 days after surgery. CCI group had the strongest staining, followed by CCI + OEC, CCI + MC-OEC, and sham groups. Scale bars: 50 μm. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell.
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Figure 3: P2X3 receptor immunoreactivity in rat L4–5 dorsal root ganglia.Immunoreactive products of the P2X3 receptor (yellow-brown, arrows), observed mainly in the cytoplasm, 7 and 14 days after surgery. CCI group had the strongest staining, followed by CCI + OEC, CCI + MC-OEC, and sham groups. Scale bars: 50 μm. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell.

Mentions: Seven and 14 days postoperatively, immunoreactive products of P2X2/3 receptors were observable as yellow-brown spots distributed mainly in the cytoplasm. The percentage and mean optical density of P2X2/3 receptor-immunoreactive cells in rat L4–5 dorsal root ganglia were lower in the sham group, and significantly higher in the CCI group (P < 0.01) at 7 and 14 days postoperatively. In the CCI + OEC group, receptor expression was lower than in the CCI group at 7 and 14 days (P < 0.05); the lowest expression was observed in the CCI + MC-OEC group, in which the percentage and optical density of P2X2/3-immunoreactive cells were significantly lower than those in the CCI + OEC and CCI groups (P < 0.05; Figures 2, 3, and Table 1).


Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury.

Zhao H, Yang BL, Liu ZX, Yu Q, Zhang WJ, Yuan K, Zeng HH, Zhu GC, Liu DM, Li Q - Neural Regen Res (2015)

P2X3 receptor immunoreactivity in rat L4–5 dorsal root ganglia.Immunoreactive products of the P2X3 receptor (yellow-brown, arrows), observed mainly in the cytoplasm, 7 and 14 days after surgery. CCI group had the strongest staining, followed by CCI + OEC, CCI + MC-OEC, and sham groups. Scale bars: 50 μm. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590250&req=5

Figure 3: P2X3 receptor immunoreactivity in rat L4–5 dorsal root ganglia.Immunoreactive products of the P2X3 receptor (yellow-brown, arrows), observed mainly in the cytoplasm, 7 and 14 days after surgery. CCI group had the strongest staining, followed by CCI + OEC, CCI + MC-OEC, and sham groups. Scale bars: 50 μm. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell.
Mentions: Seven and 14 days postoperatively, immunoreactive products of P2X2/3 receptors were observable as yellow-brown spots distributed mainly in the cytoplasm. The percentage and mean optical density of P2X2/3 receptor-immunoreactive cells in rat L4–5 dorsal root ganglia were lower in the sham group, and significantly higher in the CCI group (P < 0.01) at 7 and 14 days postoperatively. In the CCI + OEC group, receptor expression was lower than in the CCI group at 7 and 14 days (P < 0.05); the lowest expression was observed in the CCI + MC-OEC group, in which the percentage and optical density of P2X2/3-immunoreactive cells were significantly lower than those in the CCI + OEC and CCI groups (P < 0.05; Figures 2, 3, and Table 1).

Bottom Line: However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear.In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury.Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Basic Medical School, Nanchang University, Nanchang, Jiangxi Province, China ; Medical Department, Graduate School, Nanchang University, Nanchang, Jiangxi Province, China.

ABSTRACT
Olfactory bulb tissue transplantation inhibits P2X2/3 receptor-mediated neuropathic pain. However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear. In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury. We assessed mechanical nociception in the rat models 7 and 14 days after surgery by measuring paw withdrawal threshold, and examined P2X2/3 receptor expression in L4-5 dorsal root ganglia using immunohistochemistry. Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia. At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals. Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

No MeSH data available.


Related in: MedlinePlus