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Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury.

Zhao H, Yang BL, Liu ZX, Yu Q, Zhang WJ, Yuan K, Zeng HH, Zhu GC, Liu DM, Li Q - Neural Regen Res (2015)

Bottom Line: Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia.At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals.Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Basic Medical School, Nanchang University, Nanchang, Jiangxi Province, China ; Medical Department, Graduate School, Nanchang University, Nanchang, Jiangxi Province, China.

ABSTRACT
Olfactory bulb tissue transplantation inhibits P2X2/3 receptor-mediated neuropathic pain. However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear. In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury. We assessed mechanical nociception in the rat models 7 and 14 days after surgery by measuring paw withdrawal threshold, and examined P2X2/3 receptor expression in L4-5 dorsal root ganglia using immunohistochemistry. Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia. At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals. Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

No MeSH data available.


Related in: MedlinePlus

Morphology of OECs in culture and mechanical paw withdrawal threshold in rats with neuropathic pain.(A) Inverted microscope image of cells at 10 days in culture; cells were bipolar, spindle-like, or had multiple processes. Scale bar: 100 μm. (B) Fluorescence microscope image of cells at 10 days in culture; blue, nuclei (DAPI); red, p75 (OEC marker). Scale bar: 100 μm. (C) Mechanical paw withdrawal threshold was higher in the CCI + OEC and CCI + MC-OEC groups than in the CCI group. At 14 days, withdrawal threshold showed greatest improvement in the CCI + MC-OEC group. *P < 0.05, **P < 0.01, vs. sham group at the same time point; #P < 0.05, ##P < 0.01, vs. CCI group at the same time point; & P < 0.05, vs. CCI + OEC group at the same time point (one-way analysis of variance and the least significant difference post hoc test). Data are expressed as the mean ± SD; n = 6 rats per group. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell; DAPI: 4′,6-diamidino-2-phenylindole.
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Figure 1: Morphology of OECs in culture and mechanical paw withdrawal threshold in rats with neuropathic pain.(A) Inverted microscope image of cells at 10 days in culture; cells were bipolar, spindle-like, or had multiple processes. Scale bar: 100 μm. (B) Fluorescence microscope image of cells at 10 days in culture; blue, nuclei (DAPI); red, p75 (OEC marker). Scale bar: 100 μm. (C) Mechanical paw withdrawal threshold was higher in the CCI + OEC and CCI + MC-OEC groups than in the CCI group. At 14 days, withdrawal threshold showed greatest improvement in the CCI + MC-OEC group. *P < 0.05, **P < 0.01, vs. sham group at the same time point; #P < 0.05, ##P < 0.01, vs. CCI group at the same time point; & P < 0.05, vs. CCI + OEC group at the same time point (one-way analysis of variance and the least significant difference post hoc test). Data are expressed as the mean ± SD; n = 6 rats per group. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell; DAPI: 4′,6-diamidino-2-phenylindole.

Mentions: OECs cultured using Nash's differential adhesion method grew well. Under the inverted microscope, cells cultured for 10 days appeared bipolar, spindle-like or had multiple processes (Figure 1A). Immunohistochemical staining revealed that the purity of p75-positive cells was above 90% (Figure 1B).


Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury.

Zhao H, Yang BL, Liu ZX, Yu Q, Zhang WJ, Yuan K, Zeng HH, Zhu GC, Liu DM, Li Q - Neural Regen Res (2015)

Morphology of OECs in culture and mechanical paw withdrawal threshold in rats with neuropathic pain.(A) Inverted microscope image of cells at 10 days in culture; cells were bipolar, spindle-like, or had multiple processes. Scale bar: 100 μm. (B) Fluorescence microscope image of cells at 10 days in culture; blue, nuclei (DAPI); red, p75 (OEC marker). Scale bar: 100 μm. (C) Mechanical paw withdrawal threshold was higher in the CCI + OEC and CCI + MC-OEC groups than in the CCI group. At 14 days, withdrawal threshold showed greatest improvement in the CCI + MC-OEC group. *P < 0.05, **P < 0.01, vs. sham group at the same time point; #P < 0.05, ##P < 0.01, vs. CCI group at the same time point; & P < 0.05, vs. CCI + OEC group at the same time point (one-way analysis of variance and the least significant difference post hoc test). Data are expressed as the mean ± SD; n = 6 rats per group. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell; DAPI: 4′,6-diamidino-2-phenylindole.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590250&req=5

Figure 1: Morphology of OECs in culture and mechanical paw withdrawal threshold in rats with neuropathic pain.(A) Inverted microscope image of cells at 10 days in culture; cells were bipolar, spindle-like, or had multiple processes. Scale bar: 100 μm. (B) Fluorescence microscope image of cells at 10 days in culture; blue, nuclei (DAPI); red, p75 (OEC marker). Scale bar: 100 μm. (C) Mechanical paw withdrawal threshold was higher in the CCI + OEC and CCI + MC-OEC groups than in the CCI group. At 14 days, withdrawal threshold showed greatest improvement in the CCI + MC-OEC group. *P < 0.05, **P < 0.01, vs. sham group at the same time point; #P < 0.05, ##P < 0.01, vs. CCI group at the same time point; & P < 0.05, vs. CCI + OEC group at the same time point (one-way analysis of variance and the least significant difference post hoc test). Data are expressed as the mean ± SD; n = 6 rats per group. CCI: Chronic constriction injury; MC: microencapsulated; OEC: olfactory ensheathing cell; DAPI: 4′,6-diamidino-2-phenylindole.
Mentions: OECs cultured using Nash's differential adhesion method grew well. Under the inverted microscope, cells cultured for 10 days appeared bipolar, spindle-like or had multiple processes (Figure 1A). Immunohistochemical staining revealed that the purity of p75-positive cells was above 90% (Figure 1B).

Bottom Line: Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia.At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals.Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Basic Medical School, Nanchang University, Nanchang, Jiangxi Province, China ; Medical Department, Graduate School, Nanchang University, Nanchang, Jiangxi Province, China.

ABSTRACT
Olfactory bulb tissue transplantation inhibits P2X2/3 receptor-mediated neuropathic pain. However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear. In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury. We assessed mechanical nociception in the rat models 7 and 14 days after surgery by measuring paw withdrawal threshold, and examined P2X2/3 receptor expression in L4-5 dorsal root ganglia using immunohistochemistry. Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia. At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals. Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4-5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

No MeSH data available.


Related in: MedlinePlus