Limits...
Differentiation of Wharton's jelly mesenchymal stem cells into neurons in alginate scaffold.

Hosseini SM, Vasaghi A, Nakhlparvar N, Roshanravan R, Talaei-Khozani T, Razi Z - Neural Regen Res (2015)

Bottom Line: In this study, we cultured WJMSCs in a three-dimensional scaffold fabricated by 0.25% alginate and 50 mM CaCl2 in the presence of neurogenic medium containing 10 μM retinoic acid and 20 ng/mL basic fibroblast growth factor.After 10 days, immunofluorescence staining was performed for detecting β-tubulin (marker for WJMSCs-differentiated neuron) and CD271 (motor neuron marker). β-Tubulin and CD271 expression levels were significantly greater in the WJMSCs cultured in the three-dimensional alginate scaffold than in the conventional two-dimensional culture condition.These findings suggest that three-dimensional alginate scaffold cell culture system can induce neuronal differentiation of WJMSCs effectively.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Medicine Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran ; Stem Cell Laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
Alginate scaffold has been considered as an appropriate biomaterial for promoting the differentiation of embryonic stem cells toward neuronal cell lineage. We hypothesized that alginate scaffold is suitable for culturing Wharton's jelly mesenchymal stem cells (WJMSCs) and can promote the differentiation of WJMSCs into neuron-like cells. In this study, we cultured WJMSCs in a three-dimensional scaffold fabricated by 0.25% alginate and 50 mM CaCl2 in the presence of neurogenic medium containing 10 μM retinoic acid and 20 ng/mL basic fibroblast growth factor. These cells were also cultured in conventional two-dimensional culture condition in the presence of neurogenic medium as controls. After 10 days, immunofluorescence staining was performed for detecting β-tubulin (marker for WJMSCs-differentiated neuron) and CD271 (motor neuron marker). β-Tubulin and CD271 expression levels were significantly greater in the WJMSCs cultured in the three-dimensional alginate scaffold than in the conventional two-dimensional culture condition. These findings suggest that three-dimensional alginate scaffold cell culture system can induce neuronal differentiation of WJMSCs effectively.

No MeSH data available.


Related in: MedlinePlus

The percentages of CD271- and β-tubulin-positive cells in two-dimensional (2D) versus three-dimensional (3D) culture systems.The data are expressed as the mean ± SD. *P < 0.05, vs. 2D culture (Mann-Whitney U test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4590246&req=5

Figure 6: The percentages of CD271- and β-tubulin-positive cells in two-dimensional (2D) versus three-dimensional (3D) culture systems.The data are expressed as the mean ± SD. *P < 0.05, vs. 2D culture (Mann-Whitney U test).

Mentions: Immunofluorescence staining showed that WJMSCs differentiated into neurons and motor neurons in the presence of neurogenic medium (Figure 5). After exposure to neurogenic medium, the percentage of the cells expressing β-tubulin and CD271 was significantly greater in the 3D culture system than in the conventional 2D monolayer culture condition (P = 0.001 or 0.009; Figure 6).


Differentiation of Wharton's jelly mesenchymal stem cells into neurons in alginate scaffold.

Hosseini SM, Vasaghi A, Nakhlparvar N, Roshanravan R, Talaei-Khozani T, Razi Z - Neural Regen Res (2015)

The percentages of CD271- and β-tubulin-positive cells in two-dimensional (2D) versus three-dimensional (3D) culture systems.The data are expressed as the mean ± SD. *P < 0.05, vs. 2D culture (Mann-Whitney U test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590246&req=5

Figure 6: The percentages of CD271- and β-tubulin-positive cells in two-dimensional (2D) versus three-dimensional (3D) culture systems.The data are expressed as the mean ± SD. *P < 0.05, vs. 2D culture (Mann-Whitney U test).
Mentions: Immunofluorescence staining showed that WJMSCs differentiated into neurons and motor neurons in the presence of neurogenic medium (Figure 5). After exposure to neurogenic medium, the percentage of the cells expressing β-tubulin and CD271 was significantly greater in the 3D culture system than in the conventional 2D monolayer culture condition (P = 0.001 or 0.009; Figure 6).

Bottom Line: In this study, we cultured WJMSCs in a three-dimensional scaffold fabricated by 0.25% alginate and 50 mM CaCl2 in the presence of neurogenic medium containing 10 μM retinoic acid and 20 ng/mL basic fibroblast growth factor.After 10 days, immunofluorescence staining was performed for detecting β-tubulin (marker for WJMSCs-differentiated neuron) and CD271 (motor neuron marker). β-Tubulin and CD271 expression levels were significantly greater in the WJMSCs cultured in the three-dimensional alginate scaffold than in the conventional two-dimensional culture condition.These findings suggest that three-dimensional alginate scaffold cell culture system can induce neuronal differentiation of WJMSCs effectively.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran ; Cell and Molecular Medicine Student Research Group, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran ; Stem Cell Laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
Alginate scaffold has been considered as an appropriate biomaterial for promoting the differentiation of embryonic stem cells toward neuronal cell lineage. We hypothesized that alginate scaffold is suitable for culturing Wharton's jelly mesenchymal stem cells (WJMSCs) and can promote the differentiation of WJMSCs into neuron-like cells. In this study, we cultured WJMSCs in a three-dimensional scaffold fabricated by 0.25% alginate and 50 mM CaCl2 in the presence of neurogenic medium containing 10 μM retinoic acid and 20 ng/mL basic fibroblast growth factor. These cells were also cultured in conventional two-dimensional culture condition in the presence of neurogenic medium as controls. After 10 days, immunofluorescence staining was performed for detecting β-tubulin (marker for WJMSCs-differentiated neuron) and CD271 (motor neuron marker). β-Tubulin and CD271 expression levels were significantly greater in the WJMSCs cultured in the three-dimensional alginate scaffold than in the conventional two-dimensional culture condition. These findings suggest that three-dimensional alginate scaffold cell culture system can induce neuronal differentiation of WJMSCs effectively.

No MeSH data available.


Related in: MedlinePlus