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Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation.

Zhou YJ, Liu JM, Wei SM, Zhang YH, Qu ZH, Chen SB - Neural Regen Res (2015)

Bottom Line: Propofol is a neuroprotective anesthetic.Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved.These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Xingtai People's Hospital, Hebei Medical University, Xingtai, Hebei Province, China.

ABSTRACT
Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

No MeSH data available.


Related in: MedlinePlus

Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on the ultrastructure of injured rat spinal cord tissue (× 8,000).(A) Model group: A small number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (B) BMSC group and (C) propofol group: A large number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (D) Combi-nation group: a great number of regenerating axons with complete myelin sheath was observed. Arrows indicate nerve fibers.
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Figure 5: Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on the ultrastructure of injured rat spinal cord tissue (× 8,000).(A) Model group: A small number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (B) BMSC group and (C) propofol group: A large number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (D) Combi-nation group: a great number of regenerating axons with complete myelin sheath was observed. Arrows indicate nerve fibers.

Mentions: Transmission electron microscopy at 4 weeks after surgery showed that in the model group, glial scars had formed, a small amount of myelinated nerve fibers were observed in the injured rat spinal cord tissue, and macrophages had phagocytized the degenerated and necrotic myelinated nerve fibers. In the combination group, a large number of myelinated and non-myelinated nerve fibers and regenerating axons with complete myelin sheath were observed in the injured rat spinal cord tissue. The numbers of myelinated and non-myelinated nerve fibers in the injured rat spinal cord were greater in the BMSC and propofol groups than in the model group, but they were less than in the combination group (Figure 5).


Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation.

Zhou YJ, Liu JM, Wei SM, Zhang YH, Qu ZH, Chen SB - Neural Regen Res (2015)

Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on the ultrastructure of injured rat spinal cord tissue (× 8,000).(A) Model group: A small number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (B) BMSC group and (C) propofol group: A large number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (D) Combi-nation group: a great number of regenerating axons with complete myelin sheath was observed. Arrows indicate nerve fibers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590245&req=5

Figure 5: Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on the ultrastructure of injured rat spinal cord tissue (× 8,000).(A) Model group: A small number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (B) BMSC group and (C) propofol group: A large number of myelinated and non-myelinated nerve fibers were observed in the injured rat spinal cord. (D) Combi-nation group: a great number of regenerating axons with complete myelin sheath was observed. Arrows indicate nerve fibers.
Mentions: Transmission electron microscopy at 4 weeks after surgery showed that in the model group, glial scars had formed, a small amount of myelinated nerve fibers were observed in the injured rat spinal cord tissue, and macrophages had phagocytized the degenerated and necrotic myelinated nerve fibers. In the combination group, a large number of myelinated and non-myelinated nerve fibers and regenerating axons with complete myelin sheath were observed in the injured rat spinal cord tissue. The numbers of myelinated and non-myelinated nerve fibers in the injured rat spinal cord were greater in the BMSC and propofol groups than in the model group, but they were less than in the combination group (Figure 5).

Bottom Line: Propofol is a neuroprotective anesthetic.Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved.These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Xingtai People's Hospital, Hebei Medical University, Xingtai, Hebei Province, China.

ABSTRACT
Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

No MeSH data available.


Related in: MedlinePlus