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Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation.

Zhou YJ, Liu JM, Wei SM, Zhang YH, Qu ZH, Chen SB - Neural Regen Res (2015)

Bottom Line: Propofol is a neuroprotective anesthetic.Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved.These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Xingtai People's Hospital, Hebei Medical University, Xingtai, Hebei Province, China.

ABSTRACT
Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

No MeSH data available.


Related in: MedlinePlus

Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on number of fluorogold-labeled nerve fibers in the injured rat spinal cord on the cranial side.Fluorogold-labeled nerve fibers (arrows) in the injured rat spinal cord on the cranial side at 4 weeks after spinal cord injury (A, × 200) and their quantification (B). The measurement data are expressed as the mean ± SD of six rats per time point per group. One-way analysis of variance was used for comparison of the mean among groups and the least significance difference test for pairwise comparison. *P < 0.05, **P < 0.01, vs. model group; #P < 0.05, vs. BMSC group; †P < 0.05, vs. propofol group.
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Figure 4: Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on number of fluorogold-labeled nerve fibers in the injured rat spinal cord on the cranial side.Fluorogold-labeled nerve fibers (arrows) in the injured rat spinal cord on the cranial side at 4 weeks after spinal cord injury (A, × 200) and their quantification (B). The measurement data are expressed as the mean ± SD of six rats per time point per group. One-way analysis of variance was used for comparison of the mean among groups and the least significance difference test for pairwise comparison. *P < 0.05, **P < 0.01, vs. model group; #P < 0.05, vs. BMSC group; †P < 0.05, vs. propofol group.

Mentions: Retrograde labeling of the corticospinal tract revealed that the number of fluorogold-labeled nerve fibers in the injured rat spinal cord on the cranial side was significantly greater in the BMSC, propofol, and combination groups than in the model group (P < 0.05 or P < 0.01), and the number of fluorogold-labeled nerve fibers was significantly greater in the combination group than in the separate BMSC and propofol groups (P < 0.05; Figure 4). These results indicate that propofol infusion combined with BMSC transplantation promoted recovery of injured rat nerve fibers better than monotherapy.


Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation.

Zhou YJ, Liu JM, Wei SM, Zhang YH, Qu ZH, Chen SB - Neural Regen Res (2015)

Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on number of fluorogold-labeled nerve fibers in the injured rat spinal cord on the cranial side.Fluorogold-labeled nerve fibers (arrows) in the injured rat spinal cord on the cranial side at 4 weeks after spinal cord injury (A, × 200) and their quantification (B). The measurement data are expressed as the mean ± SD of six rats per time point per group. One-way analysis of variance was used for comparison of the mean among groups and the least significance difference test for pairwise comparison. *P < 0.05, **P < 0.01, vs. model group; #P < 0.05, vs. BMSC group; †P < 0.05, vs. propofol group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590245&req=5

Figure 4: Effects of propofol infusion combined with bone marrow mesenchymal stem cell (BMSC) transplantation on number of fluorogold-labeled nerve fibers in the injured rat spinal cord on the cranial side.Fluorogold-labeled nerve fibers (arrows) in the injured rat spinal cord on the cranial side at 4 weeks after spinal cord injury (A, × 200) and their quantification (B). The measurement data are expressed as the mean ± SD of six rats per time point per group. One-way analysis of variance was used for comparison of the mean among groups and the least significance difference test for pairwise comparison. *P < 0.05, **P < 0.01, vs. model group; #P < 0.05, vs. BMSC group; †P < 0.05, vs. propofol group.
Mentions: Retrograde labeling of the corticospinal tract revealed that the number of fluorogold-labeled nerve fibers in the injured rat spinal cord on the cranial side was significantly greater in the BMSC, propofol, and combination groups than in the model group (P < 0.05 or P < 0.01), and the number of fluorogold-labeled nerve fibers was significantly greater in the combination group than in the separate BMSC and propofol groups (P < 0.05; Figure 4). These results indicate that propofol infusion combined with BMSC transplantation promoted recovery of injured rat nerve fibers better than monotherapy.

Bottom Line: Propofol is a neuroprotective anesthetic.Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved.These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Xingtai People's Hospital, Hebei Medical University, Xingtai, Hebei Province, China.

ABSTRACT
Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

No MeSH data available.


Related in: MedlinePlus