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Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation.

Zhou YJ, Liu JM, Wei SM, Zhang YH, Qu ZH, Chen SB - Neural Regen Res (2015)

Bottom Line: Propofol is a neuroprotective anesthetic.Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved.These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Xingtai People's Hospital, Hebei Medical University, Xingtai, Hebei Province, China.

ABSTRACT
Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

No MeSH data available.


Related in: MedlinePlus

Morphology of bone marrow mesenchymal stem cells (BMSCs) (inverted microscope, × 200).(A) Primary BMSCs grew in a whirlpool-like pattern; (B) passage 3 BMSCs were mainly shuttle-shaped.
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Figure 1: Morphology of bone marrow mesenchymal stem cells (BMSCs) (inverted microscope, × 200).(A) Primary BMSCs grew in a whirlpool-like pattern; (B) passage 3 BMSCs were mainly shuttle-shaped.

Mentions: After 5 days of culture, BMSCs and colonies in the culture flask had obviously increased in number. Passages 1, 2, 3 BMSCs proliferated actively. After subculture, the majority of cells adhered to the culture flask and exhibited a shuttle-, polygon- or triangle-shaped appearance. BMSCs developed toward mainly shuttle shape, with two or more processes and visible nuclei and nucleoli. After reaching confluence, they grew in a whirlpool-like or parallel pattern (Figure 1). Flow cytometry showed that these cells were positive for CD29, CD105, CD44 and CD166 but negative for CD34, CD86 and CD80, with a purity of over 95%.


Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation.

Zhou YJ, Liu JM, Wei SM, Zhang YH, Qu ZH, Chen SB - Neural Regen Res (2015)

Morphology of bone marrow mesenchymal stem cells (BMSCs) (inverted microscope, × 200).(A) Primary BMSCs grew in a whirlpool-like pattern; (B) passage 3 BMSCs were mainly shuttle-shaped.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590245&req=5

Figure 1: Morphology of bone marrow mesenchymal stem cells (BMSCs) (inverted microscope, × 200).(A) Primary BMSCs grew in a whirlpool-like pattern; (B) passage 3 BMSCs were mainly shuttle-shaped.
Mentions: After 5 days of culture, BMSCs and colonies in the culture flask had obviously increased in number. Passages 1, 2, 3 BMSCs proliferated actively. After subculture, the majority of cells adhered to the culture flask and exhibited a shuttle-, polygon- or triangle-shaped appearance. BMSCs developed toward mainly shuttle shape, with two or more processes and visible nuclei and nucleoli. After reaching confluence, they grew in a whirlpool-like or parallel pattern (Figure 1). Flow cytometry showed that these cells were positive for CD29, CD105, CD44 and CD166 but negative for CD34, CD86 and CD80, with a purity of over 95%.

Bottom Line: Propofol is a neuroprotective anesthetic.Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved.These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Xingtai People's Hospital, Hebei Medical University, Xingtai, Hebei Province, China.

ABSTRACT
Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

No MeSH data available.


Related in: MedlinePlus