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Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42.

Liang ZH, Cheng XH, Ruan ZG, Wang H, Li SS, Liu J, Li GY, Tian SM - Neural Regen Res (2015)

Bottom Line: Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol).The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased.In addition, the combination of β-asarone with eugenol achieved better results than either component alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Histology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.

ABSTRACT
The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10(-10) M and 1 × 10(-5) M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

No MeSH data available.


Related in: MedlinePlus

Levels of mRNAs for Bax and Bcl-2 in PC 12 cells incubated with amyloid-beta(Aβ)42.(A) Representative agarose gel electrophoresis bands for reverse transcription PCR products showing the toxicity of Aβ42 toward PC12 cells. (B) Bax mRNA expression in PC 12 cells incubated with Aβ42. (C) Bcl-2 mRNA expression in PC 12 cells incubated with Aβ42. Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0). h: Hour(s).
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Figure 4: Levels of mRNAs for Bax and Bcl-2 in PC 12 cells incubated with amyloid-beta(Aβ)42.(A) Representative agarose gel electrophoresis bands for reverse transcription PCR products showing the toxicity of Aβ42 toward PC12 cells. (B) Bax mRNA expression in PC 12 cells incubated with Aβ42. (C) Bcl-2 mRNA expression in PC 12 cells incubated with Aβ42. Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0). h: Hour(s).

Mentions: The results of reverse transcription PCR showed that, in PC12 cells incubated with Aβ42, Bax mRNA expression increased over time and reached a peak at 24 hours, while Bcl-2 mRNA expression decreased over time and reached the lowest level at 12 and 24 hours (Figure 4).


Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42.

Liang ZH, Cheng XH, Ruan ZG, Wang H, Li SS, Liu J, Li GY, Tian SM - Neural Regen Res (2015)

Levels of mRNAs for Bax and Bcl-2 in PC 12 cells incubated with amyloid-beta(Aβ)42.(A) Representative agarose gel electrophoresis bands for reverse transcription PCR products showing the toxicity of Aβ42 toward PC12 cells. (B) Bax mRNA expression in PC 12 cells incubated with Aβ42. (C) Bcl-2 mRNA expression in PC 12 cells incubated with Aβ42. Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0). h: Hour(s).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590243&req=5

Figure 4: Levels of mRNAs for Bax and Bcl-2 in PC 12 cells incubated with amyloid-beta(Aβ)42.(A) Representative agarose gel electrophoresis bands for reverse transcription PCR products showing the toxicity of Aβ42 toward PC12 cells. (B) Bax mRNA expression in PC 12 cells incubated with Aβ42. (C) Bcl-2 mRNA expression in PC 12 cells incubated with Aβ42. Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0). h: Hour(s).
Mentions: The results of reverse transcription PCR showed that, in PC12 cells incubated with Aβ42, Bax mRNA expression increased over time and reached a peak at 24 hours, while Bcl-2 mRNA expression decreased over time and reached the lowest level at 12 and 24 hours (Figure 4).

Bottom Line: Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol).The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased.In addition, the combination of β-asarone with eugenol achieved better results than either component alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Histology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.

ABSTRACT
The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10(-10) M and 1 × 10(-5) M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

No MeSH data available.


Related in: MedlinePlus