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Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42.

Liang ZH, Cheng XH, Ruan ZG, Wang H, Li SS, Liu J, Li GY, Tian SM - Neural Regen Res (2015)

Bottom Line: Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol).The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased.In addition, the combination of β-asarone with eugenol achieved better results than either component alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Histology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.

ABSTRACT
The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10(-10) M and 1 × 10(-5) M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

No MeSH data available.


Related in: MedlinePlus

No toxicity of β-asarone (A) and eugenol (B) toward PC12 cells.Data are expressed as the mean ± SD in all cells. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test.
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Figure 2: No toxicity of β-asarone (A) and eugenol (B) toward PC12 cells.Data are expressed as the mean ± SD in all cells. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test.

Mentions: The viabilities of PC12 cells cultured with β-asarone (1 × 10-10 M to 1 × 10-5 M) and eugenol (1 × 10-10 M to 1 × 10-5 M) were similar to those of cells in the control group (P > 0.05), indicating that there were no toxic effects of β-asarone or eugenol at the experimental concentration range (Figure 2).


Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42.

Liang ZH, Cheng XH, Ruan ZG, Wang H, Li SS, Liu J, Li GY, Tian SM - Neural Regen Res (2015)

No toxicity of β-asarone (A) and eugenol (B) toward PC12 cells.Data are expressed as the mean ± SD in all cells. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590243&req=5

Figure 2: No toxicity of β-asarone (A) and eugenol (B) toward PC12 cells.Data are expressed as the mean ± SD in all cells. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test.
Mentions: The viabilities of PC12 cells cultured with β-asarone (1 × 10-10 M to 1 × 10-5 M) and eugenol (1 × 10-10 M to 1 × 10-5 M) were similar to those of cells in the control group (P > 0.05), indicating that there were no toxic effects of β-asarone or eugenol at the experimental concentration range (Figure 2).

Bottom Line: Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol).The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased.In addition, the combination of β-asarone with eugenol achieved better results than either component alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Histology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.

ABSTRACT
The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10(-10) M and 1 × 10(-5) M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

No MeSH data available.


Related in: MedlinePlus