Limits...
Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42.

Liang ZH, Cheng XH, Ruan ZG, Wang H, Li SS, Liu J, Li GY, Tian SM - Neural Regen Res (2015)

Bottom Line: Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol).The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased.In addition, the combination of β-asarone with eugenol achieved better results than either component alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Histology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.

ABSTRACT
The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10(-10) M and 1 × 10(-5) M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

No MeSH data available.


Related in: MedlinePlus

Toxic effect of amyloid-beta(Aβ)42 with different concentrations on the viability of PC12 cells as measured by MTT assay.Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4590243&req=5

Figure 1: Toxic effect of amyloid-beta(Aβ)42 with different concentrations on the viability of PC12 cells as measured by MTT assay.Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0).

Mentions: After addition of different concentrations of Aβ42 to PC12 cells, the MTT assay was applied to assess the viability of PC12 cells (Figure 1). Compared with the control group, significant differences in cell viability were observed in the Aβ 20 μM (P = 0.0067) and 30 μM (P = 0.0008) groups, while there was no significant difference in viability in the 10 μM Aβ group (P = 0.0970).


Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42.

Liang ZH, Cheng XH, Ruan ZG, Wang H, Li SS, Liu J, Li GY, Tian SM - Neural Regen Res (2015)

Toxic effect of amyloid-beta(Aβ)42 with different concentrations on the viability of PC12 cells as measured by MTT assay.Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590243&req=5

Figure 1: Toxic effect of amyloid-beta(Aβ)42 with different concentrations on the viability of PC12 cells as measured by MTT assay.Data are presented as the mean ± SD. Results were calculated from six independent experiments. Data were analyzed by one-way analysis of variance combined with the least significant difference test. **P < 0.01, vs. control group (0).
Mentions: After addition of different concentrations of Aβ42 to PC12 cells, the MTT assay was applied to assess the viability of PC12 cells (Figure 1). Compared with the control group, significant differences in cell viability were observed in the Aβ 20 μM (P = 0.0067) and 30 μM (P = 0.0008) groups, while there was no significant difference in viability in the 10 μM Aβ group (P = 0.0970).

Bottom Line: Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol).The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased.In addition, the combination of β-asarone with eugenol achieved better results than either component alone.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Histology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.

ABSTRACT
The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10(-10) M and 1 × 10(-5) M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10(-6) M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

No MeSH data available.


Related in: MedlinePlus