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Bone marrow mesenchymal stem cells transplantation promotes the release of endogenous erythropoietin after ischemic stroke.

Lv W, Li WY, Xu XY, Jiang H, Bang OY - Neural Regen Res (2015)

Bottom Line: Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats.The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group.The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.

ABSTRACT
This study investigated whether bone marrow mesenchymal stem cell (BMSC) transplantation protected ischemic cerebral injury by stimulating endogenous erythropoietin. The model of ischemic stroke was established in rats through transient middle cerebral artery occlusion. Twenty-four hours later, 1 × 10(6) human BMSCs (hBMSCs) were injected into the tail vein. Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats. Simultaneously, 3 μg/d soluble erythropoietin receptor (sEPOR) was injected into the lateral ventricle, and on the next 13 consecutive days. sEPOR blocked the release of endogenous erythropoietin. The neurogenesis in the subventricular zone was less in the hBMSCs + sEPOR group than in the hBMSCs + heat-denatured sEPOR group. The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group. The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group. These findings confirm that BMSCs contribute to neurogenesis and improve neurological function by promoting the release of endogenous erythropoietin following ischemic stroke.

No MeSH data available.


Related in: MedlinePlus

Effects of hBMSCs on neurological function in rats with ischemic stroke after blocking EPO.Long time taken to remove each stimulus from forelimbs in the adhesive-removal test and high modified neurological severity score represent severe nerve injury. Data are expressed as the mean ± SD (six rats in each group). Behavioral test was conducted in triplicate in each rat. †P < 0.05, vs. heat-denatured sEPOR group (one-way analysis of variance). tMCAo: Transient middle cerebral artery occlusion; sEPOR: soluble erythropoietin receptor; EPO: erythropoietin; hBMSCs: human bone marrow mesenchymal stem cells.
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Figure 2: Effects of hBMSCs on neurological function in rats with ischemic stroke after blocking EPO.Long time taken to remove each stimulus from forelimbs in the adhesive-removal test and high modified neurological severity score represent severe nerve injury. Data are expressed as the mean ± SD (six rats in each group). Behavioral test was conducted in triplicate in each rat. †P < 0.05, vs. heat-denatured sEPOR group (one-way analysis of variance). tMCAo: Transient middle cerebral artery occlusion; sEPOR: soluble erythropoietin receptor; EPO: erythropoietin; hBMSCs: human bone marrow mesenchymal stem cells.

Mentions: The adhesive-removal test times and mNSS score gradually decreased in the heat-denatured sEPOR, hBMSCs + heat-denatured sEPOR and hBMSCs + sEPOR groups with time. Moreover, the adhesive-removal test times and mNSS score were lower in the hBMSCs + heat-denatured sEPOR group than in the heat-denatured sEPOR-only group (P < 0.05). The adhesive-removal test time and mNSS score were similar between hBMSCs + heat-denatured sEPOR and hBMSCs + sEPOR groups (P > 0.05; Figure 2).


Bone marrow mesenchymal stem cells transplantation promotes the release of endogenous erythropoietin after ischemic stroke.

Lv W, Li WY, Xu XY, Jiang H, Bang OY - Neural Regen Res (2015)

Effects of hBMSCs on neurological function in rats with ischemic stroke after blocking EPO.Long time taken to remove each stimulus from forelimbs in the adhesive-removal test and high modified neurological severity score represent severe nerve injury. Data are expressed as the mean ± SD (six rats in each group). Behavioral test was conducted in triplicate in each rat. †P < 0.05, vs. heat-denatured sEPOR group (one-way analysis of variance). tMCAo: Transient middle cerebral artery occlusion; sEPOR: soluble erythropoietin receptor; EPO: erythropoietin; hBMSCs: human bone marrow mesenchymal stem cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590239&req=5

Figure 2: Effects of hBMSCs on neurological function in rats with ischemic stroke after blocking EPO.Long time taken to remove each stimulus from forelimbs in the adhesive-removal test and high modified neurological severity score represent severe nerve injury. Data are expressed as the mean ± SD (six rats in each group). Behavioral test was conducted in triplicate in each rat. †P < 0.05, vs. heat-denatured sEPOR group (one-way analysis of variance). tMCAo: Transient middle cerebral artery occlusion; sEPOR: soluble erythropoietin receptor; EPO: erythropoietin; hBMSCs: human bone marrow mesenchymal stem cells.
Mentions: The adhesive-removal test times and mNSS score gradually decreased in the heat-denatured sEPOR, hBMSCs + heat-denatured sEPOR and hBMSCs + sEPOR groups with time. Moreover, the adhesive-removal test times and mNSS score were lower in the hBMSCs + heat-denatured sEPOR group than in the heat-denatured sEPOR-only group (P < 0.05). The adhesive-removal test time and mNSS score were similar between hBMSCs + heat-denatured sEPOR and hBMSCs + sEPOR groups (P > 0.05; Figure 2).

Bottom Line: Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats.The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group.The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.

ABSTRACT
This study investigated whether bone marrow mesenchymal stem cell (BMSC) transplantation protected ischemic cerebral injury by stimulating endogenous erythropoietin. The model of ischemic stroke was established in rats through transient middle cerebral artery occlusion. Twenty-four hours later, 1 × 10(6) human BMSCs (hBMSCs) were injected into the tail vein. Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats. Simultaneously, 3 μg/d soluble erythropoietin receptor (sEPOR) was injected into the lateral ventricle, and on the next 13 consecutive days. sEPOR blocked the release of endogenous erythropoietin. The neurogenesis in the subventricular zone was less in the hBMSCs + sEPOR group than in the hBMSCs + heat-denatured sEPOR group. The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group. The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group. These findings confirm that BMSCs contribute to neurogenesis and improve neurological function by promoting the release of endogenous erythropoietin following ischemic stroke.

No MeSH data available.


Related in: MedlinePlus