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Bone marrow mesenchymal stem cells transplantation promotes the release of endogenous erythropoietin after ischemic stroke.

Lv W, Li WY, Xu XY, Jiang H, Bang OY - Neural Regen Res (2015)

Bottom Line: Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats.The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group.The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.

ABSTRACT
This study investigated whether bone marrow mesenchymal stem cell (BMSC) transplantation protected ischemic cerebral injury by stimulating endogenous erythropoietin. The model of ischemic stroke was established in rats through transient middle cerebral artery occlusion. Twenty-four hours later, 1 × 10(6) human BMSCs (hBMSCs) were injected into the tail vein. Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats. Simultaneously, 3 μg/d soluble erythropoietin receptor (sEPOR) was injected into the lateral ventricle, and on the next 13 consecutive days. sEPOR blocked the release of endogenous erythropoietin. The neurogenesis in the subventricular zone was less in the hBMSCs + sEPOR group than in the hBMSCs + heat-denatured sEPOR group. The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group. The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group. These findings confirm that BMSCs contribute to neurogenesis and improve neurological function by promoting the release of endogenous erythropoietin following ischemic stroke.

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Related in: MedlinePlus

Effect of hBMSCs injection on EPO levels in brain tissue of ischemic stroke rats (enzyme linked immunosorbent assay).Data are expressed as the mean ± SD (six rats in each group). Each sample was analyzed in triplicate. *P < 0.05, **P < 0.01, vs. sham group; ##P < 0.01, vs. tMCAo group (one-way analysis of variance). EPO: Erythropoietin; tMCAo: transient middle cerebral artery occlusion; hBMSCs: human bone marrow mesenchymal stem cells.
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Figure 1: Effect of hBMSCs injection on EPO levels in brain tissue of ischemic stroke rats (enzyme linked immunosorbent assay).Data are expressed as the mean ± SD (six rats in each group). Each sample was analyzed in triplicate. *P < 0.05, **P < 0.01, vs. sham group; ##P < 0.01, vs. tMCAo group (one-way analysis of variance). EPO: Erythropoietin; tMCAo: transient middle cerebral artery occlusion; hBMSCs: human bone marrow mesenchymal stem cells.

Mentions: ELISA results demonstrated that at 14 days after surgery, the endogenous EPO levels from ischemic tissues were higher in the tMCAo group than in the sham group (P < 0.05). Endogenous EPO levels were significantly higher in the hBMSCs group (more than twice) than in the tMCAo group and sham group (P < 0.01; Figure 1).


Bone marrow mesenchymal stem cells transplantation promotes the release of endogenous erythropoietin after ischemic stroke.

Lv W, Li WY, Xu XY, Jiang H, Bang OY - Neural Regen Res (2015)

Effect of hBMSCs injection on EPO levels in brain tissue of ischemic stroke rats (enzyme linked immunosorbent assay).Data are expressed as the mean ± SD (six rats in each group). Each sample was analyzed in triplicate. *P < 0.05, **P < 0.01, vs. sham group; ##P < 0.01, vs. tMCAo group (one-way analysis of variance). EPO: Erythropoietin; tMCAo: transient middle cerebral artery occlusion; hBMSCs: human bone marrow mesenchymal stem cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590239&req=5

Figure 1: Effect of hBMSCs injection on EPO levels in brain tissue of ischemic stroke rats (enzyme linked immunosorbent assay).Data are expressed as the mean ± SD (six rats in each group). Each sample was analyzed in triplicate. *P < 0.05, **P < 0.01, vs. sham group; ##P < 0.01, vs. tMCAo group (one-way analysis of variance). EPO: Erythropoietin; tMCAo: transient middle cerebral artery occlusion; hBMSCs: human bone marrow mesenchymal stem cells.
Mentions: ELISA results demonstrated that at 14 days after surgery, the endogenous EPO levels from ischemic tissues were higher in the tMCAo group than in the sham group (P < 0.05). Endogenous EPO levels were significantly higher in the hBMSCs group (more than twice) than in the tMCAo group and sham group (P < 0.01; Figure 1).

Bottom Line: Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats.The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group.The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.

ABSTRACT
This study investigated whether bone marrow mesenchymal stem cell (BMSC) transplantation protected ischemic cerebral injury by stimulating endogenous erythropoietin. The model of ischemic stroke was established in rats through transient middle cerebral artery occlusion. Twenty-four hours later, 1 × 10(6) human BMSCs (hBMSCs) were injected into the tail vein. Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats. Simultaneously, 3 μg/d soluble erythropoietin receptor (sEPOR) was injected into the lateral ventricle, and on the next 13 consecutive days. sEPOR blocked the release of endogenous erythropoietin. The neurogenesis in the subventricular zone was less in the hBMSCs + sEPOR group than in the hBMSCs + heat-denatured sEPOR group. The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group. The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group. These findings confirm that BMSCs contribute to neurogenesis and improve neurological function by promoting the release of endogenous erythropoietin following ischemic stroke.

No MeSH data available.


Related in: MedlinePlus