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Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction.

Ahn JY, Tae HJ, Cho JH, Kim IH, Ahn JH, Park JH, Kim DW, Cho JH, Won MH, Hong S, Lee JC, Seo JY - Neural Regen Res (2015)

Bottom Line: c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders.Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions.The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, South Korea ; Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.

ABSTRACT
c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction.

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Western blot analysis of c-Fos protein in the cingulate and piriform cortices of the sham-operated (sham) and myocardial infarction rats at 3, 7, 14, 28 and 56 days after myocardial infarction.The c-Fos protein expression was represented as the relative optical density (ROD) of target immunoblot band to sham band (n = 7 rats per group). *P < 0.05, vs. sham group; one-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).
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Figure 6: Western blot analysis of c-Fos protein in the cingulate and piriform cortices of the sham-operated (sham) and myocardial infarction rats at 3, 7, 14, 28 and 56 days after myocardial infarction.The c-Fos protein expression was represented as the relative optical density (ROD) of target immunoblot band to sham band (n = 7 rats per group). *P < 0.05, vs. sham group; one-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).

Mentions: Western bolt analysis showed that the change pattern in c-Fos protein level in the cingulate and piriform cortices after MI was similar to the immunohistochemical data (Figure 6). c-Fos protein level in these areas was increased from 3 days and peaked at 7 and 14 days in the cingulate and piriform cortices, respectively, after MI. Thereafter, c-Fos protein levels was decreased with time after MI, and c-Fos protein level was not different from that in the sham-operated group at 56 days after MI (Figure 6).


Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction.

Ahn JY, Tae HJ, Cho JH, Kim IH, Ahn JH, Park JH, Kim DW, Cho JH, Won MH, Hong S, Lee JC, Seo JY - Neural Regen Res (2015)

Western blot analysis of c-Fos protein in the cingulate and piriform cortices of the sham-operated (sham) and myocardial infarction rats at 3, 7, 14, 28 and 56 days after myocardial infarction.The c-Fos protein expression was represented as the relative optical density (ROD) of target immunoblot band to sham band (n = 7 rats per group). *P < 0.05, vs. sham group; one-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4590237&req=5

Figure 6: Western blot analysis of c-Fos protein in the cingulate and piriform cortices of the sham-operated (sham) and myocardial infarction rats at 3, 7, 14, 28 and 56 days after myocardial infarction.The c-Fos protein expression was represented as the relative optical density (ROD) of target immunoblot band to sham band (n = 7 rats per group). *P < 0.05, vs. sham group; one-way analysis of variance and a Tukey's post hoc test were used. The bars indicate the mean ± SEM. d: Day(s).
Mentions: Western bolt analysis showed that the change pattern in c-Fos protein level in the cingulate and piriform cortices after MI was similar to the immunohistochemical data (Figure 6). c-Fos protein level in these areas was increased from 3 days and peaked at 7 and 14 days in the cingulate and piriform cortices, respectively, after MI. Thereafter, c-Fos protein levels was decreased with time after MI, and c-Fos protein level was not different from that in the sham-operated group at 56 days after MI (Figure 6).

Bottom Line: c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders.Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions.The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medicine, Sacred Heart Hospital, College of Medicine, Hallym University, Anyang, South Korea ; Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, South Korea.

ABSTRACT
c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction.

No MeSH data available.


Related in: MedlinePlus